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http://dx.doi.org/10.35399/ISK.33.2.7

Genetic Diversity and Relationship of the Genus Barbatula (Cypriniformes; Nemacheilidae) by Mitochondrial DNA Cytochrome b Partial Gene in Korea  

An, Jung-Hyun (Division of Life Sciences, College of Life Sciences, Incheon National University)
Yu, Jeong-Nam (Biodiversity Research Team, Nakdonggang National Institute of Biological Resources)
Kim, Byung-Jik (Biological Resources Research Department, National Institute of Biological Resources)
Bae, Yang-Seop (Division of Life Sciences, College of Life Sciences, Incheon National University)
Publication Information
Korean Journal of Ichthyology / v.33, no.2, 2021 , pp. 107-116 More about this Journal
Abstract
Two stone loaches (Nemacheilidae, Cypriniformes), Barbatula toni (Dybowski, 1869) and B. nuda (Bleeker, 1864), have been recognized in the Korean waters to date. Recently, due to indiscriminate artificial introduction as well as the change of their habitats induced by natural disasters, it seems to be concerned about the damage of species-specific geographic boundaries. We examined the genetic difference of two Korean Barbatula species by the haplotype network based on the Cytochrome b sequences of mitochondrial DNA and the phylogenetic relationships among them including Barbatula fishes occurring around the Korean peninsula. As a result, three and 29 haplotypes were obtained from B. toni and B. nuda, respectively, and totally three clades comprising "toni group", "nuda hangang group", and "nuda donghae group" were identified. The sequence variable sites among them was 10~24%, showing a difference of interspecific level. Phylogenetic relationships of the latter group, especially, forms an independent cluster discriminating with other two groups as well as the Chinese, Japanese, Russian, and European Barbatula species, suggesting the possibility of the specific level divergence.
Keywords
Barbatula; toni; nuda; Cytochrome b; haplotype; Korea;
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