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http://dx.doi.org/10.35399/ISK.31.4.7

The Anaesthetic Effect of MS-222 for Longtooth Grouper, Epinephelus moara and the Hybrid Grouper, E. moara ♀ × E. lanceolatus ♂  

Park, Jong Yeon (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University)
Kim, Kang-Rae (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University)
Bang, In-Chul (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.31, no.4, 2019 , pp. 235-240 More about this Journal
Abstract
The anaesthetic effect of tricaine methanesulfonate (MS-222) concentrations and water temperatures for longtooth grouper (Epinephelus moara) and hybrid grouper (E. moara ♀ × E. lanceolatus ♂) were investigated. Anesthetic induction and recovery time were measured at 18, 22, 26 and 30℃ of Cwater temperature and 100, 150, 200 and 250 ppm of anesthetic concentrations. Anesthetic induction time tended to decrease with increasing concentration and water temperature. Recovery time was proportional to concentration, but inversely proportional to water temperature. However, there was no significant differences in recovery time at 22℃ or lower. The optimal anesthesia condition was 30℃ and 100 ppm, which was the shortest recovery time for longtooth grouper, and 150 ppm at 30℃ in the case of hybrid grouper because anesthetic time is significantly different with 100 ppm in spite of no significant differences with 100 ppm for recovery time. As a results of two-way ANOVA test, there was a significant difference between the species of longtooth and hybrid grouper. On the other hand, there was no interaction effect between concentration and species. Also, there was no interaction effect among species, concentration, and water temperature.
Keywords
Anaesthetics; tricaine methanesulfonate; hybrid grouper; Epinephelus lanceolatus; Epinephelus moara;
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