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Profiles of Glucocorticoid Receptor mRNA Expression and Physiological Changes in Response to Osmotic and Thermal Stress Conditions in Black Porgy (Acanthopagrus schlegeli)  

An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University)
Shin, Hyun-Suk (Division of Marine Environment & Bioscience, Korea Maritime University)
Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research & Development Institute)
Kil, Gyung-Suk (Division of Electrical & Electronic Engineering, Korea Maritime University)
Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
Korean Journal of Ichthyology / v.22, no.1, 2010 , pp. 17-24 More about this Journal
Abstract
The present study investigated the expression of glucocorticoid receptor (GR) mRNA as a stress response during salinity changes (35, 10, and 0 psu) and water temperature changes (from $20^{\circ}C$ to $30^{\circ}C$, $1^{\circ}C$/day) in black porgy. We cloned the full-length GR cDNA from the kidney and examined its expression in the gill, kidney, and intestine by quantitative real-time PCR (QPCR) during salinity and water temperature changes. During salinity changes, the levels of GR mRNA in the gill, kidney, and intestine were highest at 0 psu, and the levels of plasma cortisol and glucose were elevated, but triiodothyronine ($T_3$) decreased. Also, during water temperature changes, the levels of GR mRNA in the gill, kidney, and intestine increased at $30^{\circ}C$. Plasma parameters also increased with an increase in water temperature. Therefore, this upregulation of GR mRNA was a good indicator of stress, such as those resulting from changes in salinity and water temperature.
Keywords
Black porgy; glucocorticoid receptor; salinity change; water temperature change;
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