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Genetic Variability of Farmed Olive Flounder (Paralichthys olivaceus) Populations Managed with no Consideration of Genetic Diversity  

Noh, Jae Koo (Genetics and Breeding Research Center, NFRDI)
Kim, Hyun Chul (Genetics and Breeding Research Center, NFRDI)
Park, Choul Ji (Genetics and Breeding Research Center, NFRDI)
Lee, Jeong-Ho (Genetics and Breeding Research Center, NFRDI)
Kim, Jong-Hyun (Genetics and Breeding Research Center, NFRDI)
Lee, Mi-Sug (Genetics and Breeding Research Center, NFRDI)
Kim, Woo-Jin (Biotechnology Research Center, NFRDI)
Kim, Kyung-Kil (Biotechnology Research Center, NFRDI)
Myeong, Jeong-In (Genetics and Breeding Research Center, NFRDI)
Publication Information
Korean Journal of Ichthyology / v.20, no.4, 2008 , pp. 248-254 More about this Journal
Abstract
Olive flounder (Paralichthys olivaceus) is one of the most popular farmed fish in Korea. Genetic variability of the fish was investigated by means of microsatellite DNA markers. All of the 8 microsatellite loci were analyzed in this study. For the confirmation of genetic variation during a shift in generation, microsatellite variability was compared within the same hatchery strains but produced in different spawning years. When genetic variability of farmed flounders produced in 2006 and 2007 was compared with that of 2003, a marked reduction of genetic variability was observed in the 2006 and 2007 populations. Mean number of alleles per locus and expected mean heterozygosity decreased from 9.75 and 0.796 (in 2003 population) to 7.78 and 0.785 (in 2006 population), respectively. Moreover, we have observed the distortion of allele frequency. These results show that reduced genetic variability of farmed olive flounder in processed generation has lower numbers of alleles and genetic variability than these of wild fish. Our results suggest that to have a sustainable aquaculture of this species, there is need for scientific broodstock management based on genetic variation and more intensive breeding practices to improve genetic diversity and to avoid detrimental inbreeding effects.
Keywords
Paralichthys olivaceus; olive flounder; genetic variability; microsatellite;
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