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Genetic Variability and Population Structure of Olive Flounder Paralichthys olivaceus from Stocked Areas Using Microsatellite DNA Markers  

Jeong, Da Sang (Marine Seed Releasing Technology Center, NFRDI)
Jeon, Chang Young (Marine Seed Releasing Technology Center, NFRDI)
Publication Information
Korean Journal of Ichthyology / v.20, no.3, 2008 , pp. 156-162 More about this Journal
Abstract
Five microsatellite DNA markers were used to investigate genetic diversity and population structure of olive flounder Paralichthys olivaceus collected from four locations (YD, SC, GJ, WD) where hatchery-based seeds of the flounder have been released. The average of observed (Ho) and expected heterozygosity (He) ranged from 0.833 to 0.871, and from 0.842 to 0.876, respectively. The average number of alleles per locus ranged from 12.4 to 17.8. The proportion of stocked flounder ranged from 20.0% to 95.8% for wild-caught populations with a decreasing tendency of alleles per locus following a higher proportion of stocked flounder. There is need to implement a more careful stock-enhancement program of hatchery-based seeds and to monitor its genetic effects on wild populations to ensure conservation of natural flounder resources.
Keywords
Flounder; Microsatellite DNA marker; Genetic variation; Population structure;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Booke, H.E. 1999. The stock concept revisited: perspectives on its history in fisheries. Fish. Res., 43: 9-11   DOI   ScienceOn
2 Evans, B., J. Bartlett, N. Sweijd, P. cook and N.G. Elliott. 2004. Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae). Aquaculture, 233: 109-127   DOI   ScienceOn
3 FAO. 1993. Report of the expert consultation on utilization and conservation of aquatic genetic resources. FAO Fish. Tech. Rep., 491: 1-58
4 Li, Q., C. Park, T. Endo and A. Kijima. 2004. Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai). Aquaculture, 235: 207-222   DOI   ScienceOn
5 MOMAF. 2007. Annual report of fisheries in Korea. MOMAF, 77-78
6 Sekino, M., K. Saitoh, T. Yamada, M. Hara and Y. Yamashita. 2005. Genetic tagging of released Japanese flounder (Paralichthy olivaceus) based on polymorphic DNA markers. Aquaculture, 244: 49-61   DOI   ScienceOn
7 Sekino, M., M. Hara and N. Taniguchi. 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthy olivaceus. Aquaculture, 213: 101-122   DOI   ScienceOn
8 Norris, A.T., D.G. Bradley and E.P. Cunningham. 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 182: 73-83   DOI   ScienceOn
9 Jeong, D.S., K.S. Kim and K.K. Kim. 2006. Evaluation of effective breeders number (Ne) for stock enhancement in olive flounder Paralichthys olivaceus using microsatellite DNA markers. J. of Aquaculture, 19: 205-209   과학기술학회마을
10 Hara, M. and M. Sekino. 2003. Efficient detection of parentage in a cultured Japanese flounder Paralichthy olivaceus using microsatellite DNA marker. Aquaculture, 217: 107-114   DOI   ScienceOn
11 Tessier, N., L. Bernatchez and J.M. Wright. 1997. Population structure and impact of supportive breeding inferred from mito chondrial and microsatellite DNA analyses in land-locked Atlantic salmon Salmo salar L. Mol. Ecol., 6: 735-750   DOI   ScienceOn
12 Kim, W.J., K.K. Kim, J.H. Lee, D.W. Park, J.Y. Park and J.Y. Lee. 2003. Isolation and characterization of polymorphic microsatellite loci in the olive flounder (Paralichthys olivaceus). Molecular Ecology Notes, 3: 491-493   DOI   ScienceOn
13 Yoshida, K., M. Takagi, M. Tanaka and N. Taniguchil. 2000. Genetic variability and divergence of wild and artificially raised Japanese flounder Paralichthys olivaceus inferred from microsatellite DNA analysis. Fish Gen. Breeed. Sci., 29: 93-102
14 Tamura, K. and M. Nei. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10: 512-526
15 Jeong, D.S., E.B. Gonzalez, K. Morishima, K. Arai and T. Umino. 2007. Parentage assignment of stocked black sea bream, Acanthopagrus schlegeli in Hiroshima Bay using microsatellite DNA markers. Fish. Sci., 73: 823-830   DOI   ScienceOn
16 Liu Y., S. Chen and B. Li. 2005. Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Aquaculture, 243: 103-111   DOI   ScienceOn
17 Perez-Enriquez, R., M. Takagi and N. Taniguchi. 1999. Genetic change and pedigrees tracing of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement, based on microsatellite NDA markers. Aquaculture, 173: 413-423   DOI   ScienceOn
18 Sekino, M., K. Saitoh, T. Yamada, A. Kumagai, M. Hara and Y. Yamashita. 2003. Microsatellite-based pedigree tracing in a Japanese flounder Paralichthy olivaceus hatchery strain: implications for hatchery management related to stock enhancement program. Aquaculture, 221: 255-263   DOI   ScienceOn