Browse > Article

Effects of Cryoprotectants and Diluents on Cryopreservation of the Red Seabream, Pagrus major Sperm  

Kho, Kang Hee (Faculty of Marine Technology, Chonnam National University)
Publication Information
Korean Journal of Ichthyology / v.19, no.2, 2007 , pp. 173-177 More about this Journal
Abstract
A series of experiments were conducted to compare the effects of various diluents and cryoprotectants on the motility and survival rate in cryopreservation of the red seabream, Pagrus major sperm. Sperm was efficiently cryopreserved using 300 mM glucose as a diluent. Two cryoprotectant, dimethyl sulfoxide (DMSO) and glycerol, were added to 300 mM glucose to formulate the extenders at concentrations between 5% and 30% by volume for freezing. The highest post-thawed sperm motility and survival rate were obtained with 10% DMSO.
Keywords
Pagrus major; cryopreservation; cryoprotectants; diluents; dimethyl sulfoxide;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Blom, E. 1950. A one-minute live-dead sperm stain by means of eosin-nigrosin. Fertil. Steril., 1 : 176-177   DOI
2 Guest, W.C., J.W. Avault and J.D. Roussel. 1976. Preservation of channel catfish sperm. Trans. Am. Fish. Soc., 3 : 469-474
3 Gwo, J.C. 1994. Cryopreservation of yellowfin seabream (Acanthopagrus latus) spermatozoa. Therigenology, 41 : 989-1004   DOI   ScienceOn
4 Lahnsteiner, F., B. Berger, T. Wiesmann and R. Patzner. 1996. Changes in morphology, physiology, metabolism, and fertilization capacity of rainbow trout semen following cryopreservation. Prog. Fish. Cult., 58 : 149-159   DOI   ScienceOn
5 Morisawa, M. and S. Morisawa. 1990. In 'Control of sperm motility: Biological and clinical aspects' Ed by C. Gagnon, CRC press, Boca Raron, pp. 137-152
6 Strussmann, C.A., P. Renard, H. Ling and F. Takashima. 1994. Motility of pejerrey, Odontesthes bonariensis spermatozoa. Fish. Sci., 60 : 9-13   DOI
7 Watson, P.E. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and assessment of their post thawing function. Reprod. Fertil. Dev., 7 : 871-891   DOI
8 Yao, Z., L.W. Crim, G.F. Richardson and C.J. Emerson. 2000. Motility, fertility and ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after cryopreservation. Aquaculture, 181 : 361-375   DOI   ScienceOn
9 Morris, G.J. 1981. Liposomes as a model system for investigating freezing injury. (in) Morris G.J. and A. Clarke, ed. Effects of low temperatures on biological membranes. Academic, New York, pp. 214-262
10 Zar, J.H., 1984. Biostatistical Analysis. Prentice-Hall Inc. Englewood Cliffs, N.J., pp. 620
11 Fribourgh, J.H. 1966. The application of a differential staining method to low-temperature studies on goldfish spermatozoa. Prog. Fish-Cult., 28 : 227-231   DOI
12 Morisawa, M. 1985. Initiation mechansim of sperm motility at spawning in teleosts. Zool. Sci., 2 : 605-615
13 Fabbrocini, A.S., S. Lubrano Lavadera, S. Rispoli and G. Sansone. 2000. Cryopreservation of seabream (Sparus aurata) spermatozoa. Cryobiology, 40 : 46-53   DOI   ScienceOn
14 Kuwano, K. and N. Saga, 2000. Cryopreservation of marine algae: Cryopreservation of marine algae: Applications in biotechnology. In: Figerman, M. and R. Nagabhusshanam. ed. Recent advances in marine biotechnology. Volume 4 : Aquaculture. Part A, Seaweeds and invertebrates invertebrates. Science Pubulishers Inc, New Hampshire pp. 23-40
15 Ohta, H. and T. Izawa. 1996. Diluent for cool storage of Japanese eel (Anguilla japonicus) spermatozoa. Aquaculture, 142 : 107-118   DOI   ScienceOn
16 Blaxter, J.H.S. 1953. Sperm storage and cross fertilization of spring and autumn spawning herring. Nature, 172
17 Chang, Y.J. 1997. Present and future studies on the cryo-preservation of fish gametes. Suisanzoshoku, 45 : 557-564
18 Gwo, J.C. 1993. Cryopreservaion of black grouper (Epinephelus malabaricus) spermatozoa. Therigenology, 39 : 1331-1342   DOI   ScienceOn
19 Gwo, J.C., K. Strawn, M.T. Longnecker and R. Connie 1991. Cryopreservation of atlantic croaker spermatozoa. Aquaculture, 94 : 355-375   DOI   ScienceOn
20 Lim, H.K., Y.J. Chang and P.K. Jo. 2003. Effects of cryopreservations on survival and hatching of black seabream, Acanthopagrus schlegeli Embryos. J. of Aquaculture, 16(4) : 262-266   과학기술학회마을
21 Kang K.H., K.H. Kho, Z.T. Chen, J.M. Kim, Y.H. Kim and Z.F. Zhang. 2004. Cryopreservation of filefish (Thamnaconus septentrionalis Gunther, 1877) sperm. Aquaculture research, 35(15) : 1429-1433   DOI   ScienceOn
22 Chao, N.H., H.P. Tsai and I.C. Liao. 1992. Short- and longterm cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Schneider). Asian Fish. Sci., 5 : 103-116
23 Kho, K.H., M. Morisawa and K.S. Choi. 2005. Cell signaling mechanisms of sperm motility in aquatic species. J. Microbiol. Biotechnol., 15(3) : 665-671   과학기술학회마을