Browse > Article

Survey of Genes Responsive to Long-Term Heat Stress Using a cDNA Microarray Analysis in Mud Loach (Misgurnus mizolepis) Liver  

Cho, Young Sun (Department of Aquaculture, Pukyong National University)
Lee, Sang Yoon (Department of Aquaculture, Pukyong National University)
Noh, Choong Hwan (Marine Resources and Research Department, Korea Ocean Research and Development Institute)
Nam, Yoon Kwon (Institute of Marine Living Modified Organisms (iMLMO) Pukyong National University)
Kim, Dong Soo (Institute of Marine Living Modified Organisms (iMLMO) Pukyong National University)
Publication Information
Korean Journal of Ichthyology / v.18, no.2, 2006 , pp. 65-77 More about this Journal
Abstract
Gene transcripts potentially responsive to the heat stress were surveyed by cDNA microarray analysis in mud loach (Misgurnus mizolepis). Transcriptional profiles of hepatic tissue in the fish exposed to either $23^{\circ}C$ or $32^{\circ}C$ for 4 weeks were compared each other by 3 replicated hybridization assays using 1,124 unigene clones selected from mud loach liver expressed sequence tags (ESTs). A total of 93 clones showed the substantially increased mRNA levels (>2-fold) in $32^{\circ}C$-exposed group when compared in $23^{\circ}C$control group. It includes various enzymes and proteins involved in energy pathway, protease/protein metabolisms, immune/antioxidant functions, cytoskeleton/cell structure, transport and/or signal transduction. Maximum level of increase was up to 15-fold relative to $23^{\circ}C$ treatment. Heat exposure also resulted in the significant decrease (less than 50% relative to $23^{\circ}C$-exposed fish) of the transcriptional activities in 85 genes. Besides the above categories, yolk protein (vitellogenin) and ribosomal proteins were notably down regulated in the fish exposed to heat stress. A number of novel gene transcripts were also detected in both up-regulated and down-regulated groups.
Keywords
mud loach; heat stress; gene expression; cDNA microarray;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cho, Y.S., B.N. Choi, K.-H. Kim, S.K. Kim, D.S. Kim, I.C. Bang and Y.K. Nam. 2006. Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnatus fasciatus). Aquaculture, 253 : 667-679   DOI   ScienceOn
2 Gordon, C.J. and L.R. Leon. 2005. Thermal stress and the physiological response to environmental toxicants. Rev. Environ. Health, 20 : 235-263
3 Groothuis, T. and J. Neefjes. 2005. The ins and outs of intracellular peptides and antigen presentation by MHC class I molecules. Curr. Top Microbiol. Immunol., 300 : 127-148   DOI
4 Imai, H. and Y. Nakagawa. 2003. Biological significance of phospholipid hydroperoxide glutathione peroxidase (PHGPx, GPx4) in mammalian cells. Free Radic. Biol. Med., 34 : 145-169   DOI   ScienceOn
5 King, L.S., D. Kozono and P. Agre. 2004. From structure to disease: the evolving tale of aquaporin biology. Nat. Rev. Mol. Cell. Biol., 5 : 687-698   DOI   ScienceOn
6 Nam, Y.K. and D.S. Kim. 2002. Screening of potential stress-responsive and immune-related genes by expressed sequence tags in mud loach (Misgurnus mizolepis). J. Fish Pathol., 15 : 83-92
7 Peng, Y., P.H. Yang, J.A. Tanner, J.D. Huang, M. Li, H.F. Lee, R.H. Xu, H.F. Kung and M.C. Lin. 2006. Coldinducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis. Biochem. Biophys. Res. Commun. (in press)
8 Robertson, J.C. and J.R. Hazel. 1995. Cholesterol content of trout plasma membranes varies with acclimation temperature. Am. J. Physiol., 269 : 1113-1119
9 Sawaguchi, S., H. Kagawa, N. Ohkubo, N. Hiramatsu, C.V. Sullivan and T. Matsubara T. 2006. Molecular characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream (Pagrus major), a marine teleost spawning pelagic eggs. Mol. Reprod. Dev., 73 : 719-736   DOI   ScienceOn
10 Schipper, R.G. and A.A. Verhofstad. 2002. Distribution patterns of ornithine decarboxylase in cells and tissues: facts, problems, and postulates. J. Histochem. Cytochem., 50 : 1143-1160   DOI   ScienceOn
11 Weinstein, D.A., C.E. Correia, A.C. Saunders and J.I. Wolfsdorf. 2006. Hepatic glycogen synthase deficiency: an infrequently recognized cause of ketotic hypoglycemia. Mol. Genet. Metab., 87 : 284-288   DOI   ScienceOn
12 Hazel, J.R. 1984. Effects of temperature on the structure and metabolism of cell membranes in fish. Am. J. Physiol., 246 : 460-470
13 Borgnia, M., S. Nielsen, A. Engel and P. Agre. 1999. Cellular and molecular biology of the aquaporin water channels. Annu. Rev. Biochem., 68 : 425-458   DOI   ScienceOn
14 Hwang, J.Y., T. Ohira, I. Hirono and T. Aoki. 2004. A poreforming protein, perforin, from a non-mammalian organism, Japanese flounder, Paralichthys olivaceus. Immunogenetics, 56 : 360-367
15 Ogden, C.A. and K.B. Elkon. 2006. Role of complement and other innate immune mechanisms in the removal of apoptotic cells. Curr. Dir. Autoimmun., 9 : 120-142
16 Parihar, M.S., A.K. Dubey, T. Faveri and P. Prakash. 1996. Changes in lipid peroxidation, superoxide dismutase activity, ascorbic acid and phospholipids content in liver of freshwater catfish Heteropneustes fossilis exposed to elevated temperature. J. Therm. Biol., 21 : 323-330   DOI   ScienceOn
17 Rus, H., C. Cudrici and F. Niculescu. 2005. The role of the complement system in innate immunity. Immunol. Res., 33 : 103-112   DOI   ScienceOn
18 Boshra, H., J. Li and J.O. Sunyer. 2006. Recent advances on the complement system of teleost fish. Fish Shellfish Immunol., 20 : 239-262   DOI   ScienceOn
19 Catalfamo, M and P.A. Henkart. 2003. Perforin and the granule exocytosis cytotoxicity pathway. Curr. Opin. Immunol., 15 : 522-527   DOI   ScienceOn
20 Schulte, P.M., H.C. Glemet, A.A. Fiebig and D.A. Powers. 2000. Adaptive variation in lactate dehydrogenase-B gene expression: Role of a stress-responsive regulatory element. Proc. Natl. Acad. Sci. USA, 97 : 6597-6602
21 Jack, D.L. and M.W. Turner. 2003. Anti-microbial activities of mannose-binding lectin. Biochem. Soc. Trans., 31 : 753-757   DOI
22 Lushchak, V.I. and T.V. Bagnyukova. 2006. Temperature increase results in oxidative stress in goldfish tissues. 2. Antioxidant and associated enzymes. Comp. Biochem. Physiol., C 143 : 36-41
23 Zelko, I.N., T.J. Mariani and R.J. Folz. 2002. Superoxide dismutase multigene family: a comparison of the CuZn- SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic. Biol. Med., 33 : 337-349   DOI   ScienceOn
24 Deneka, M., M. Neeft and P. van der Sluijs. 2003. Regulation of membrane transport by rab GTPases. Crit. Rev. Biochem. Mol. Biol., 38 : 121-142   DOI   ScienceOn
25 Malik, S. 2003. Transcriptional regulation of the apolipoprotein AI gene. Front. Biosci., 8 : 360-368   DOI
26 Fujita, T. 2002. Evolution of the lectin-complement pathway and its role in innate immunity. Nat. Rev. Immunol., 2 : 346-353   DOI   ScienceOn
27 Johnston, I.A. and J. Dunn. 1987. Temperature acclimation and metabolism in ectotherms with particular reference to teleost fish. Symp. Soc. Exp. Biol., 41 : 67-93
28 Pegg, A.E. 2006. Regulation of ornithine decarboxylase. J. Biol. Chem. (in press)
29 Deane, E.E. and N.Y. Woo. 2005. Cloning and characterization of the hsp70 multigene family from silver sea bream: Modulated gene expression between warm and cold temperature acclimation. Biochem. Biophys. Res. Commun., 330 : 776-783   DOI   ScienceOn
30 Hanumanthaiah, R., K. Day and P. Jagadeeswaran. 2002. Comprehensive analysis of blood coagulation pathways in teleostei: evolution of coagulation factor genes and identification of zebrafish factor VIIi. Blood Cells Mol. Dis., 29 : 57-68   DOI   ScienceOn
31 Ojima, N., M. Yamashita and S. Watabe. 2005. Comparative expression analysis of two paralogous Hsp70s in rainbow trout cells exposed to heat stress. Biochim. Biophys. Acta, 1681 : 99-106   DOI   ScienceOn
32 Zehmer, J.K. and J.R. Hazel. 2005. Thermally induced changes in lipid composition of raft and non-raft regions of hepatocyte plasma membranes of rainbow trout. J. Exp. Biol., 208 : 4283-4290   DOI   ScienceOn
33 Hascilowicz, T., N. Murai, S. Matsufuji and Y. Murakami. 2002. Regulation of ornithine decarboxylase by antizymes and antizyme inhibitor in zebrafish (Danio rerio). Biochim. Biophys. Acta, 1578 : 21-28   DOI   ScienceOn
34 Nikoskelainen, S., J. Lehtinen and E.M. Lilius. 2002. Bacteriolytic activity of rainbow trout (Oncorhynchus mykiss) complement. Dev. Comp. Immunol., 26 : 797-804   DOI   ScienceOn
35 Vornanen, M., M. Hassinen, H. Koskinen and A. Krasnov. 2005. Steady-state effects of temperature acclimation on the transcriptome of the rainbow trout heart. Am. J. Physiol. Regul. Integr. Comp. Physiol., 289 : 1177- 1184   DOI
36 Larsson, D.G., I. Mayer, S.J. Hyllner and L. Forlin. 2002. Seasonal variations of vitelline envelope proteins, vitellogenin, and sex steroids in male and female eelpout (Zoarces viviparus). Gen. Comp. Endocrinol., 125 : 184-196   DOI   ScienceOn
37 Rabergh, C.M., S. Airaksinen, A. Soitamo, H.V. Bjorklund, T. Johansson, M. Nikinmaa and L. Sistonen. 2000. Tissue-specific expression of zebrafish (Danio rerio) heat shock factor 1 mRNAs in response to heat stress. J. Exp. Biol., 203 : 1817-1824
38 Gerlach, G.F., L. Turay, K.T. Malik, J. Lida, A. Scutt and G. Goldspink. 1990. Mechanisms of temperature acclimation in the carp: a molecular biology approach. Am. J. Physiol., 259 : 237-244
39 Siddiq, A., I.A. Ayoub, J.C. Chavez, L. Aminova, S. Shah, J.C. LaManna, S.M. Patton, J.R. Connor, R.A. Cherny, I. Volitakis, A.I. Bush, I. Langsetmo, T. Seeley, V. Gunzler and R.R. Ratan. 2005. Hypoxia-inducible factor prolyl 4-hydroxylase inhibition-A target for neuroprotection in the central nervous system. J. Biol. Chem., 280 : 41732-41743   DOI   ScienceOn
40 Kim, B.H. and A. Takemura. 2003. Culture conditions affect induction of vitellogenin synthesis by estradiol- 17 beta in primary cultures of tilapia hepatocytes. Comp. Biochem. Physiol., B 135 : 231-239   DOI   ScienceOn
41 Oakley, A.J. 2005. Glutathione transferases: new functions. Curr. Opin. Struct. Biol., 15 : 716-723   DOI   ScienceOn
42 Harari, P.M., D.J. Fuller and E.W. Gerner. 1989. Heat shock stimulates polyamine oxidation by two distinct mechanisms in mammalian cell cultures. Int. J. Radiat. Oncol. Biol. Phys., 16 : 451-457   DOI   ScienceOn
43 Seelen, M.A., A. Roos and M.R. Daha. 2005. Role of complement in innate and autoimmunity. J. Nephrol., 18 : 642-653
44 Farout, L. and B. Friguet. 2006. Proteasome function in aging and oxidative stress: implications in protein maintenance failure. Antioxid. Redox Signal., 8 : 205- 216   DOI   ScienceOn
45 Jordens, I., M. Marsman, C. Kuijl and J. Neefjes. 2005. Rab proteins, connecting transport and vesicle fusion. Traffic, 6 : 1070-1077   DOI   ScienceOn
46 Pan, F., J. Zarate, A. Choudhury, R. Rupprecht and T.M. Bradley. 2004 Osmotic stress of salmon stimulates upregulation of a cold inducible RNA binding protein (CIRP) similar to that of mammals and amphibians. Biochimie, 86 : 451-461   DOI   ScienceOn
47 Buda, C., I. Dey, N. Balogh, L.I. Horvath, K. Maderspach, M. Juhasz, Y.K. Yeo and T. Farkas. 1994. Structural order of membranes and composition of phospholipids in fish brain cells during thermal acclimatization. Proc. Natl. Acad. Sci. USA., 91 : 8234-8238
48 Fujita, T., M. Matsushita and Y. Endo. 2004. The lectincomplement pathway-its role in innate immunity and evolution. Immunol. Rev., 198 : 185-202   DOI   ScienceOn
49 Dey, I., C. Buda, T. Wiik, J.E. Halver and T. Farkas. 1993. Molecular and structural composition of phospholipid membranes in livers of marine and freshwater fish in relation to temperature. Proc. Natl. Acad. Sci. USA., 90 : 7498-7502
50 Heise, K., S. Puntarulo, M. Nikinmaa, D. Abele and H.O. Portner. 2006. Oxidative stress during stressful heat exposure and recovery in the North Sea eelpout Zoarces viviparus L. J. Exp. Biol., 209 : 353-363   DOI   ScienceOn