Browse > Article
http://dx.doi.org/10.17820/eri.2022.9.3.183

Evaluation of Growth Inhibition for Microcystis aeruginosa with Ultrasonic Irradiation Time  

Kang, Eun Byeol (Department of Civil and Environmental Engineering, Hanbat National University)
Joo, Jin Chul (Department of Civil and Environmental Engineering, Hanbat National University)
Jang, So Ye (Environmental Engineering, Hanbat National University)
Go, Hyeon Woo (Department of Civil and Environmental Engineering, Hanbat National University)
Park, Jung Su (Department of Civil and Environmental Engineering, Hanbat National University)
Jeong, Moo Il (Adsonic)
Lee, Dong Ho (Department of Mobile Convergence Engineering, Hanbat National University)
Publication Information
Ecology and Resilient Infrastructure / v.9, no.3, 2022 , pp. 183-193 More about this Journal
Abstract
The growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was evaluated using a large algae sample volume (10 L) for various ultrasonic irradiation times (0.5, 1, 1.5, 2, 2.5 and 3 hr) at a laboratory scale. Based on the analysis of Chl-a and cell number of M. aerginosa, algae growth inhibition was observed with the decrease in Chl-a and cell number in all experimental groups after the ultrasonic irradiation. For the experimental group (T_B, T_C, T_D) with an ultrasonic irradiation time of less than 2 hours, rapid regrowth of algae was observed after growth inhibition, but the experimental group (T_E, T_F, T_G) with an irradiation time of more than 2 hours successfully inhibited algal growth lasting one or two more days. Based on the comparison of the recovery time to initial cell number the experimental group (T_B, T_C, T_D) took less than 20 days whereas the experimental group (T_E, T_F, T_G) took about 30 days. Correspondingly, the experimental group showed a high first order decay rate (𝜅) in proportion to the ultrasonic irradiation time during the growth inhibition period. Additionally, the specific growth rates (𝜇) during regrowth in the experimental group with irradiation time of more than 2 hours were relatively low compared to those in the experimental group with less than 2 hours. Therefore, ultrasonic irradiation for more than 2 hours is required for long-term (30 days) inhibition of algal growth in stagnant waters. However, the appropriate ultrasonic irradiation time for algae growth inhibition should be determined according to various field conditions such as the volume of stagnant water, water depth, flow rate, algae concentration, etc. Finally, damages to the algal cell surface and cell membrane were clearly observed, and both destruction and disturbance of gas vesicles of M. aeruginosa in the experimental group were discovered, indicating the growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was confirmed.
Keywords
Algae growth inhibition; Irradiation time; Microcystis aeruginosa; Regrowth; Ultrasonic irradiation;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Park, J., Church, J., Son, Y., Kim, K.T., and Lee, W.H. 2017. Recent advances in ultrasonic treatment: challenges and field applications for controlling harmful algal blooms (HABs). Ultrasonics Sonochemistry 38: 326-334.   DOI
2 Park, J., Son, Y., and Lee, W.H. 2019. Variation of efficiencies and limits of ultrasonication for practical algal bloom control in fields. Ultrasonics Sonochemistry 55: 8-17.   DOI
3 Peng, Y., Zhang, Z., Kong, Y., Li, Y., Zhou, Y., Shi, X., and Shi, X. 2020. Effects of ultrasound on Microcystis aeruginosa cell destruction and release of intracellular organic matter. Ultrasonics Sonochemistry 63: 104909.   DOI
4 Purcell, D., Parsons, S.A., and Jefferson, B. 2013. The influence of ultrasound frequency and power, on the algal species Microcystis aeruginosa, Aphanizomenon flos-aquae, Scenedesmus subspicatus and Melosira sp. Environmental Technology 34(17): 2477-2490.   DOI
5 Sim, J.H., Seo, H.J., and Kwon, B.D. 2006. Study on the Efficiency of Algae Removal Using Ultrasonic Waves in Double Cisterns. Journal of Korean Society of Environmental Engineers 28(12): 1310-1315.
6 Song, I.S. 2014. Dometstic and overseas algae control technology, Konetic report, 2-10.
7 Srisuksomwong, P., Whangchai, N., Yagita, Y., Okada, K., Peerapornpisal, Y., and Nomura, N. 2011. Effects of ultrasonic irradiation on degradation of microcystin in fish ponds. International Journal of Agriculture and Biology, 13(1).
8 Thackeray, S.J., Jones, I.D., and Maberly, S.C. 2008. Long-term change in the phenology of spring phytoplankton: species-specific responses to nutrient enrichment and climatic change. Journal of Ecology 96(3): 523-535.   DOI
9 Li, Y., Shi, X., Zhang, Z., and Peng, Y. 2019. Enhanced coagulation by high-frequency ultrasound in Microcystis aeruginosa-laden water: Strategies and mechanisms. Ultrasonics Sonochemistry 55: 232-242.   DOI
10 Lee, T.J., Nakano, K., and Matsumura, M. 2000. A new method for the rapid evaluation of gas vacuoles regeneration and viability of cyanobacteria by flow cytometry. Biotechnology Letters 22(23): 1833-1838.   DOI
11 Ma, B., Chen, Y., Hao, H., Wu, M., Wang, B., Lv, H., and Zhang, G. 2005. Influence of ultrasonic field on microcystins produced by bloom-forming algae. Colloids and Surfaces B: Biointerfaces 41(2-3), 197-201.   DOI
12 Oh, H.M., Lee, S.J., Kim, J.H., Kim, H.S., and Yoon, B.D. 2001. Seasonal variation and indirect monitoring of microcystin concentrations in Daechung Reservoir, Korea. Applied and Environmental Microbiology 67(4): 1484-1489.   DOI
13 Paerl, H.W. and Barnard, M.A. 2020. Mitigating the global expansion of harmful cyanobacterial blooms: Moving targets in a human-and climatically-altered world. Harmful Algae 96: 101845.   DOI
14 Zhang, G., Zhang, P., Wang, B., and Liu, H. 2006. Ultrasonic frequency effects on the removal of Microcystis aeruginosa. Ultrasonics Sonochemistry 13(5): 446-450.   DOI
15 Kim, M.K., Moon, B., Kim, T.K., and Zoh, K.D. 2015. A study on production & removal of microcystin, taste & odor compounds from algal bloom in the water treatment processes. The Korean Journal of Public Health 52(1): 33-42.
16 Codd, G.A., Morrison, L.F., and Metcalf, J.S. 2005. Cyanobacterial toxins: risk management for health protection. Toxicology and Applied Pharmacology 203(3): 264-272.   DOI
17 Harke, M.J., Steffen, M.M., Gobler, C.J., Otten, T.G., Wilhelm, S.W., Wood, S.A., and Paerl, H.W. 2016. A review of the global ecology, genomics, and biogeography of the toxic cyanobacterium, Microcystis spp. Harmful Algae 54: 4-20.   DOI
18 Jang, S.Y., Joo, J.C., Kang, E.B., Go, H.W., Park, J., Jeong, M.I., and Lee, D.H. 2022. Derivation of Ultrasonic Irradiation Condition to Inhibit the Growth of Microcystis Aeruginosa. Journal of Korean Society of Environmental Engineers, Eng 44(4): 101-110.   DOI
19 Lee, H.J., Park, H.K., Heo, J., Lee, H.J., and Hong, D.G. 2018. Colonial Cyanobacteria, Microcystis Cell Density Variations using Ultrasonic Treatment. Journal of Korean Society on Water Environment 34(2): 210-215.   DOI
20 Li, P., Song, Y., and Yu, S. 2014. Removal of Microcystis aeruginosa using hydrodynamic cavitation: performance and mechanisms. Water Research 62: 241-248.   DOI
21 Meerhoff, M., Audet, J., Davidson, T.A., De Meester, L., Hilt, S., Kosten, S., ... and Jeppesen, E. 2022. Feedback between climate change and eutrophication: revisiting the allied attack concept and how to strike back. Inland Waters 1-18.
22 Srisuksomwong, P., Peerapornpisal, Y., Nomura, N., and Whangchai, N. 2012. Comparative ultrasonic irradiation efficiency of Microcystis aeruginosa and M. wesenbergii from surface bloom and re-flotation behavior. Chiang Mai J. Sci 39(4): 731-735.
23 Park, H.K., Kim, H., Lee, J.J., Lee, J.A., Lee, H., Park, J.H., ... and Moon, J. 2011. Investigation of criterion on harmful algae alert system using correlation between cell numbers and cellular microcystins content of Korean toxic cyanobacteria. Journal of Korean Society on Water Environment 27(4): 491-498.   DOI
24 Park, Y.M., Kwon, O.C., Park, J.W., Chung, G.Y., Lee, J.E., and Seo, E.W. 2013. Effects of low powered ultrasonic wave exposure on microcystis sp. (cyanobacteria). Korean Journal of Environmental Biology 31(2): 113-120.   DOI
25 Rajasekhar, P., Fan, L., Nguyen, T., and Roddick, F.A. 2012. Impact of sonication at 20 kHz on Microcystis aeruginosa, Anabaena circinalis and Chlorella sp. Water Research 46(5): 1473-1481.   DOI
26 Wu, X., Joyce, E.M., and Mason, T.J. 2012. Evaluation of the mechanisms of the effect of ultrasound on Microcystis aeruginosa at different ultrasonic frequencies. Water Research 46(9): 2851-2858.   DOI
27 Ahn, C.Y., Park, M.H., Joung, S.H., Kim, H.S., Jang, K.Y., and Oh, H.M. 2003. Growth inhibition of cyanobacteria by ultrasonic radiation: laboratory and enclosure studies. Environmental Science & Technology 37(13): 3031-3037.   DOI
28 Byeon, M.S., Byun, J.H., Im, J.K., Jin, Y.H., Noh, H.R., Kim, G.S., ... and You, S.J. 2018. Molecular Biological Characteristics of Cyanobacteria Originated Off-flavor in Water (I). Han-River Water Environment Research Center National Institute of Environmental Research.
29 Chen, G., Ding, X., and Zhou, W. 2020. Study on ultrasonic treatment for degradation of Microcystins (MCs). Ultrasonics Sonochemistry 63: 104900.   DOI
30 Dehghani, M.H. 2016. Removal of cyanobacterial and algal cells from water by ultrasonic waves-A review. Journal of Molecular Liquids 222: 1109-1114.   DOI
31 Hallegraeff, G., Enevoldsen, H., and Zingone, A. 2021. Global harmful algal bloom status reporting. Harmful Algae 102: 101992.   DOI
32 Jachlewski, S., Botes, M., and Cloete, T.E. 2013. The effect of ultrasound at 256 KHz on Microcystis aeruginosa, with and without gas vacuoles. Water SA 39(1): 171-172.
33 Jang, S.Y., Joo, J.C., Kang, E.B., Ahn, C.M., Park, J., Jeong, M.I., and Lee, D.H. 2021. Evaluation of Growth Inhibition for Microcystis aeruginosa with Different Frequency of Ultrasonic Devices. Ecology and Resilient Infrastructure 8(3): 143-153.   DOI
34 KICT. 1997. Research on optimal water quality management of Ilsan Lake
35 Kim, G.Y., Joo, J.C., Lee, M.J., Park, J.R., Ahn, C.H., and Lee, S. 2019. Evaluation on Growth Inhibition Effect of Harmful Blue Green Algae Using TiO 2-embedded Expanded Polystyrene (TiEPS) Balls: Lab-scale Indoor/ Outdoor Experiments. Journal of Korean Society Environmental Engineers 41(11): 637-646.   DOI
36 Kong, Y., Peng, Y., Zhang, Z., Zhang, M., Zhou, Y., and Duan, Z. 2019. Removal of Microcystis aeruginosa b y ultrasound: Inactivation mechanism and release of algal organic matter. Ultrasonics Sonochemistry 56: 447-457.   DOI
37 Lee, C.S., Ahn, C.Y., La, H.J., Lee, S., and Oh, H.M. 2013. Technical and strategic approach for the control of cyanobacterial bloom in fresh waters. Korean Journal of Environmental Biology 31(4): 233-242.   DOI
38 Lee, K.H. and Lee, S.H. 2012. Monitoring of floating green algae using ocean color satellite remote sensing. Journal of the Korean Association of Geographic Information Studies 15(3): 137-147.   DOI