[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.3839/jksabc.2008.058

A Simple and Rapid Method for Screening of Nocardioides simplex Mutant with High Cholesterol Oxidase Activity

Jang, Young-Sun (Ben's Lab Co., Ltd.)
Jeong, Jong-Moon (Department of Life Science, University of Suwon)

Publication Information

Applied Biological Chemistry / v.51, no.4, 2008 , pp. 338-341 More about this Journal

Keywords

cholesterol oxidase; ethyl methanesulfonate; Nocardioides simplex; mutagenesis; screening method;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)

Reference

1	Cheethan PSJ, Dunnill P, and Lilly MD (1982) The characterization and interconversion of three forms of cholesterol oxidase extracted from Nocardia rhodochrous. Biochem J 201, 515-521 DOI
2	Doukyu N and Aono R (2001) Cloning, sequence analysis and expression of a gene encoding an organic solventand detergent-tolerant cholesterol oxidase of Burkholdera cepacia strain ST-200. Appl Microbiol Biotechnol 57, 146-152 DOI
3	Johnson TL and Somkuti GA (1991) Isolation of cholesterol oxidase from Rhodococcus equi ATCC 33706. Biotechnol Appl Biochem 13, 196-204
4	Liu WH, Meng MH, and Chen KS (1988) Purification and some properties of cholesterol oxidases produced by an inducible and a constitutive mutant of Arthrobacter simplex. Agric Biol Chem 52, 413-418
5	Masurekar PS and Goodhue CT (1978) United States Patent No. 4,093,517. Eastman Kodak Company. Rochester. NY
6	Shirokane Y, Nakamura K, and Mizusawa K (1977) Purification and some properties of an extracellular 3 $\beta$ -hydroxysteroid oxidase produced by Corynebacterium cholesterolicum. J Ferment Technol 55, 337-346
7	Xiang J and Sampson NS (2004) Library screening studies to investigate substrate specificity in the reaction catalyzed by cholesterol oxidase. Protein Eng Des Sel 17, 341-348 DOI ScienceOn
8	Fukuyama M and Miyake Y (1979) Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin. J Biochem 85, 1183-1193
9	Lee SY, Rhee HI, Tae WC, Shin JC, and Park BK (1989) Purification and characterization of cholesterol oxidase from Pseudomonas sp. and taxonomic study of the strain. Appl Microbiol Biotechnol 31, 542-546 DOI
10	Murooka Y and Yamashita M (2001) Genetic and protein engineering of diagnostic enzymes, cholesterol oxidase and xylitol oxidase. J Biosci Bioeng 91, 433-441 DOI
11	Doukyu N and Aono R (1998) Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200. Appl Environ Microbiol 64, 1929-1932
12	H, Kag H, Kagawa M, and Nakamura S (1976) Some enzymatic properties of 3 $\beta$ -hydroxysteroid oxidase produced by Streptomyces violascens. J Biochem 79, 903-915 DOI
13	Nishiya Y, Harada N, Teshima SI, Yamashita M, Fujii I, Hirayama N, and Murooka Y (1997) Improvement of thermal stability of Streptomyces cholesterol oxidase by random mutagenesis and a structural interpretation. Protein Eng 10, 231-235 DOI
14	Arlma K, Nagasawa M, Bae M, and Tamura G (1969) Microbial transformation of steroids. Part I: Decomposition of cholesterol by microorganisms. Agric Biol Chem 33, 1636-1643
15	Inouye Y, Taguchi K, Fuhii A, Ishimaru K, Nakamura S, and Nomi R (1982) Purification and characterization of extracellular 3a-hydroxysteroid oxidase produced by Streptoverticillium cholesterolicum. Chem Pharm Bull 30, 951-958 DOI
16	Uwajima T, Yagi H, and Terada O (1974) Properties of crystalline 3 $\beta$ -hydroxysteroid oxidase of Brevibacterium sterolicum. Agric Biol Chem 38, 1149-1156 DOI
17	Allain CC, Poon LS, Chan CSG, Richmond W, and Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20, 470-475