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Purification and Characteristics of Tyrosinase Inhibitor Produced by Actinomycetes F-97  

Bang, Byung-Ho (Division of Food Science, Eulji University)
Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology)
Kim, Jin-O (Department of Microbial Engineering, Konkuk University)
Yi, Dong-Heui (Department of Microbial Engineering, Konkuk University)
Publication Information
Applied Biological Chemistry / v.51, no.3, 2008 , pp. 153-158 More about this Journal
Abstract
An actinomycetes F-97 producing tyrosinase inhibitor was isolated from soil samples. Isolation and purification of tyrosinase inhibitor produced by F-97 was performed as follows: IRC-120 ($NH_4^+$ type) column chromatography, silica gel column chromatography, $C_{18}$ column chromatography and Sephadex LH-20 column chromatography were used successively after the centrifuged supernatant was adjusted to pH 4.0. To identify the purity of the inhibitor, octadecylsilyl(ODS) HPLC was carried out with 5% methanol as a mobile phase. Finally, the purification yield of a tyrosinase inhibitor was 5.24%. The inhibitor was very soluble in water, methanol and ethanol but insoluble in acetone, butanol, ethylacetate and chloroform. The ${\lambda}_{max}$ value of this inhibitor in water was 194nm under UV light. The biochemical test of the inhibitor was positive in Molish, Benedict, cone. $H_2SO_4$, and $KMnO_4$ tests but negative in iodine, ninhydrin, Million, Sakaguchi, xanthoproteic and Emerson tests. The tyrosinase inhibitor was stable against heat treatment of $100^{\circ}C$ for 50 minutes and pH $4{\sim}9$. The $IC_{50}$ value of this inhibitor was $19.2{\mu}g/ml$ for mushroom tyrosinase. In $1,000{\mu}g/ml$ inhibitor concentration, inhibition zone was 27 mm for Streptomyces bikiniensis NRRL B-1049. The inhibition of F-97 against mushroom tyrosinase was competitive with tyrosine.
Keywords
Actinomycetes; inhibitor; melanin; tyrosinase;
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Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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