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Cytotoxic and Anti-inflammatory Activities of Lipids from the Nuruk (Rhizopus oryzae KSD-815)  

Kwak, Ho-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Lee, Sang-Jin (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Research Laboratories, Kooksoondang Brewery Co. Ltd.)
Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Bae, Nark-Hyun (Department of Genetic Engineering, Sungkyunkwan University)
Jung, La-Koon (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Hong, Sung-Youl (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Gye-Won (Research Laboratories, Kooksoondang Brewery Co. Ltd.)
Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Applied Biological Chemistry / v.51, no.2, 2008 , pp. 142-147 More about this Journal
Abstract
Nuruk is the Korean traditional Koji that contains various microorganisms and has been used to make the traditional fermented foods including alcoholic beverages. Rhizopus oryzae KSD-815 was isolated from the alcohol-fermenting Nuruk used for manufacturing traditional alcohol. In this study, the authors reported the isolation and identification of four lipids from the Nuruk (Rhizopus oryzae KSD-815) that inoculated wheat with Rhizopus oryzae KSD-815. The dried and powdered Nuruk (Rhizopus oryzae KSD-815) were extracted three times at room temperature with 80% aqueous MeOH. The extracts were partitioned with EtOAc, n-BuOH, and water, successively. The EtOAc extract was suspended in 80% MeOH and partitioned repeatedly with n-hexane. From the n-hexane fraction, four lipids were isolated through the repeated silica gel and ODS column chromatographies. According to the results of physico-chemical data including NMR, GC and MS, the chemical structures of the compounds were determined as linolenic acid methyl ester (1), palmitic acid methyl ester (2), linoleic acid (3), palmitic acid (4). Cytotoxicity was evaluated in huamn breast cancer cells, MDA-MB-231 and human hepatocarcinoma, SK-HEP-1 cells using MTT assay. Exposure of compounds 1 and 3 led to a dose-dependent inhibition of cell viability in both cancer cell lines. In addition, treatment of RAW264.7 cells with compound 3 caused inhibition of lipopolysaccharide/interferon-${\gamma}$-induced nitric oxide production.
Keywords
anti-inflammation; cytotoxicity; linoleic acid; linolenic acid methyl ester; Nuruk; palmitic acid methyl ester; Rhizopus oryzae;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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