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Immunostimulating and Anticancer Activities of Hot Water Extract from Capsosiphon fulvescens  

Park, Hee-Yeon (Biotechnology Research Center, National Fisheries Research & Development Institute)
Lim, Chi-Won (Biotechnology Research Center, National Fisheries Research & Development Institute)
Kim, Yeon-Kye (Biotechnology Research Center, National Fisheries Research & Development Institute)
Yeon, Ho-Dong (Food Sanitation Team, National Fisheries Research & Development Institute)
Lee, Ka-Jung (Food Sanitation Team, National Fisheries Research & Development Institute)
Publication Information
Applied Biological Chemistry / v.49, no.4, 2006 , pp. 343-348 More about this Journal
Abstract
To investigate the immunostimulating and anticancer activities from hot water extract of Capsosiphon fulvescens, tumor cell toxicity, sarcoma-180 growth inhibition activity, complement system-activity, intestinal immune system and oral toxicity were performed. The extract of Capsosiphon fulvescens was prepared by hot water and precipitated by using ethanol. Partially purified extract (CFE) was obtained after dialysis and ultrafiltration. The polysaccharide compositions consisted of xylose(19.1%), fucose(15.3%), mannose(4.2%) and galactose(7.9%). The tumor cell toxicity of CFE slightly showed at high concentrations of 10-30 ${\mu}g/ml$, but inhibition ratio against mouse solid tumor was more increased for CFE of 40.1-59.4% than the control. Blood leukocyte counts increased to a maximum of 83% including liver, spleen and thymic of mouse. Immunoglobulin A binding amounts showed a high level of CFE of $2,454{\pm}113.8-2,670{\pm}133.1{\mu}g/mg$ in comparison with the control of $2,092{\pm}123.0{\mu}g/mg$. Acute toxicity of CFE was not detected at the concentration of 2,000 mg/kg in normal mouse.
Keywords
immunostimulating activity; anticancer activity; capsosiphon fulvescens;
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1 Gao, Q. P., Kiyohara, H. and Yamada, H. (1990) Further structural studies of anti-complementary acidic heteroglycans from the leaves of Panax ginseng C.A. Meyer. Carbohydr. Res. 196, 111-125   DOI   ScienceOn
2 Gao, Q. P., Kiyohara, H., Cyong J. C. and Yamada, H. (1988) Characterization of anti- complementary acidic heteroglycans from the leaves of Panax ginseng C.A. MEYER. Carbohydr. Res. 181, 175-187   DOI   ScienceOn
3 Mayer, M. M., Miller, J. A. and Shin, H. S. (1970) A specific method for purification of the second component of guinea pig complement and a chemical evaluation of the one-hit theory. J. Immunol. 105, 327-341
4 Gao, Q. P., Kiyohara, H., Cyong, J. C. and Yamada, H. (1989) Chemical properties and anti-complementary activities of polysaccharides fractions from roots and leaves of Panax ginseng C. A. Meyer. Planta Med. 55, 9-13   DOI
5 Arthur, C. and Guyton, M. D. (1986) In Textbook of medical physiology pp. 51-59, W. B. Saunder Company, New York
6 Kweon, M. H., Kim, H .I. Sung, H. J. and Yang, H. C. (1994) Core structure of the anti-complementary acidic polysaccharide (PA-IIa-1) from water extract of pteridium aquilinum var. latiusculum. Food Biotech. 3, 137-143
7 Chaplin, M. F. (1982) A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography. Anal. Biochem. 123, 336-341   DOI   ScienceOn
8 Yamada, H., Kiyohara, H., Cyong, J. C. and Otsuka, Y. (1987) Characterization of anti-complementary acidic heteroglycans from the seed of Coix lachryma jobi var. ma-yuen. Phytochemistry 26, 3269-3275   DOI   ScienceOn
9 Yoichi, F., Yoichi, K., Koko, M., Jun-ichi, K. and Tomotari, M. (1999) Effect of bifidobacteria feeding on fecal flora and production of immunoglobulins in lactating mouse. Int. J. Food Microbiol. 46, 193-197   DOI   ScienceOn
10 Yamada, H. and Kiyohara, H. (1989) Bioactive polysaccharides from Chinese herbal medicines. Abstract of Chinese Medicines 3, 104-111
11 Low, S. K. A. and Reid, K. B. M. (1988) Complement. In complement, pp. 1-21. IRL Press, Oxford
12 Kweon, M. H., Sung, H. C. and Yang, H. C. (1994) Acidic heteroglycans with anti-complementary activity from the water extract of Pteridium aquilinum var. latiusculum. Food Biotech. 3, 83-91
13 Yamada, H. and Kiyohara, H. (1989) Structure of the neutral carbohydrate side chains in anti-complementary acidic polysaccharides from the root of Angelica acutiloba Kitagawa. Carbohydr. Res. 187, 255-265   DOI   ScienceOn
14 Yamata, H., Ra, K. S., Kiyohara, H., Cyong, J. C., Yang, H. C. and Otsuka, Y. (1988) Characterization of ant-complementary neutral polysaccharides from the roots of Bupleurm falcatum. Phytochemistry 27, 3163-3171   DOI   ScienceOn
15 Shin, K. S., Cho, H. Y., Sung, H. C. and Yang, H. C. (1992) Action mode of the anti-complementary polysaccharide purified from Arecae Pericarpium. J. Korean Agric. Chem. Soc. 35, 462-470
16 Yamada, H., Nagai, T., Cyong, J. C. and Otsuka, Y. (1991) Mode of complement activation by acidic heteroglycans from the leaves of Artemisia princeps PAMP. Chem. Pharm. Bull. 39, 2077-2081   DOI
17 Kiyohara H. and Yamada, H. (1989) Structure of an anticomplementary arabinogalactan from the root of Angelica acutiloba Kitagawa. Carbohydr. Res. 193, 173-192   DOI   ScienceOn
18 Zhao, I. F., Kiyohar, H., Matsumoto, T. and Yamada, H. (1993) Anti-complementary acidic polysaccharides from roots of Lithosperum euchromum. Phytochemistry 34, 719-724   DOI   ScienceOn
19 Cho, K. J., Lee, Y. S. and Ryu, B. H. (1990) Antitumor effect and immunologt activity of seaweeds toward sarcoma-180. Bull. Korean Fish. Soc. 23, 345-352
20 Yamada, H., Kiyohara, H., Cyong, J. C., Kojima, Y., Kumazawa, Y. and Otsuka, Y. (1984) Studies on polysaccharides from Anglelica acutiloba. Planta medica 50, 163-167   DOI   ScienceOn
21 Ra, K. S. (1990) Purification and chemical properties of anticomplementary polysaccharides from the Roots of Bupleurum falcatum L. and the fruits of Capsicum annuum L., Ph.D. Thesis, Korea University, Seoul, Korea