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Characterization of Phytase Produced by Streptomyces sp. YB-26  

Yoon, Ki-Hong (School of Food Science & Biotechnology, Woosong University)
Publication Information
Applied Biological Chemistry / v.48, no.4, 2005 , pp. 311-314 More about this Journal
Abstract
Approximately twelve hundred strains of Actonomycetes isolated from domestic soli were tested for their ability to produce extracellular phytase. Of all these isolates a strain, YB-26, that had the highest potential for phytase activity was chosen. The nucleotide sequence of 16S rDNA of the isolate YB-26 showed the highest similarity to that of strains beloning to genus Streptomyces. The partially purified extracellular phytase was obtained from the culture filtrate of Streptomyces sp. YB-26 grown on GSM broth by ammonium sulfate precipitation (15-70%), DEAE-Sepharose column and Q-Sepharose column chromatography. The partially purified enzyme showed the maximum activity for hydrolysis of phyate at $60^{\circ}C$ and pH 7.0, and retained 90% of its maximum activity at the range of pH $6.0{\sim}8.0$. It was thermolabile and its thermostability did not increase in the presence of calcium chloride.
Keywords
Streptomyces; phytase; reaction property;
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