Browse > Article

Antimicrobial Effects and Antioxidative Activity of Baek-bu-ja(Aconiti koreani Rhizoma) by Extraction Solvent Ratio  

Yoon, So-Jung (Department of Food Engineering Sangju National University)
Kim, Jeung-Hoan (Department of Food Engineering Sangju National University)
Lee, Kyoung-Hwan (Department of Food Engineering Sangju National University)
Kwon, Hyo-Jung (Department of Food Engineering Sangju National University)
Chun, Sung-Sook (Department of Food Science & Technology Yeungnam University)
Cho, Young-Je (Department of Food Engineering Sangju National University)
Publication Information
Applied Biological Chemistry / v.48, no.3, 2005 , pp. 258-262 More about this Journal
Abstract
For the purpose of developing natural antioxidant, the antioxidative activity and antimicrobial of phenolics isolated from Baek-bu-ja (Aconiti koreani Rhizoma) were determined. Optimum extracting condition for phenolics were water extracts. HPLC analysis showed that the four major phenolic metabolites were rosemarinic, protocatechuic, caffeic and chlorogenic acids. The water extracts of Baekbuja did not have antimicrobial activity against H. pylori; however, the ethanol extracts revealed higher antimicrobial activity. Electron donation ability on DPPH of Baekbuja ethanol extract was 20% higher than other ethanol extracts. The 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical decolorization (ABTS) and antioxidant protection factor (PF) were determined with extracts from Aconiti koreani Rhizoma. 94% inhibition and 1.14 PF were shown on ABTS and antioxidant protection factor with 60% ethanol extracts. Also, TBARS (thiobarbituric acid reactive substances) showed $0.19\;{\mu}M$ in the control and $0.07\;{\mu}M$ in the 80% ethanol extracts. The result suggests that Baekbuja extract may be useful as potential sources of anti Helicobacter pylori, antioxidant.
Keywords
Baekbuja; Aconiti koreani Rhizoma; Helicobacter pylori; antioxidant;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zielinski, H. and Kolzolwska, H. (2000) Antioxidant activity and total penolics in selected cereal grains and their different morphological fractions. J. Agric. Food Chem. 48, 2008-2016   DOI   ScienceOn
2 Yang, C. Y. (1995) In Poisonous drug medical herb, chinese public opinion crane publishing company, Beijing, pp.191-197
3 Park, S. Y., Chung, B. S., Lee, H. K., Lee, H. S., Ryu, J. H. (1989) Studies on the Preparation of Processde Aconiti Tubers. Kor. J. Pharmacogn. 20, 25-31
4 Devy, C. and Gautier, R. (1990) New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochem. Parmacol. 39, 399-405   DOI   ScienceOn
5 Folin, O. and Denis, W. (1912) On phosphotungasticphosphomolybdis compounds as color reagents. J. Biol. Chem. 12, 239-249
6 Im, R. J. (1998) In Flora medica coreana: Part traditional medicine (3nd ed.) Pyongyang, Seoul, pp. 171-172
7 Pellegrini, N., Roberta, R., Min, Y. and Catherine, R. E. (1998) Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activities appplying 2,2'-Azinibis (3- ehtylenebenzothizaoline-6-sulfonic acid) fadical cation decolorization assay. Method Enzymol. 299, 379-389
8 Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200
9 Torel J., Gillard J. and Gillard P. (1996) Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochem. 25, 383-385
10 Kang, Y. H., Park, Y. K., Oh, S. R. and Moon, K. D. (1995) Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food. Sci. Technol. 27, 978-984
11 Andarwulan, N., Fariaz, D., Wattimena, G. A. and Shetty, K. (1999) Antioxidant activity associated with lipid and phenolic mobilization during seed germination of Pangium edule Reinw. J. Agric. Food Chem. 47, 3158-3163   DOI   ScienceOn
12 Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C. and Lee, B. Y. (1995) Antioxidative activity and physiological activity of some korean medicinal plants. Korean J. Food Sci. Technol. 27, 80- 85
13 Brieskom, C. H., Fuch, A., Bredenberg, J. B., McChensney, J. D., Wenkert, E. (1964) The structure of carnosol. J. Org. Chem. 29, 2293-2297   DOI
14 Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C., Lee, B. Y. (1995) Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J. Food. Sci. Technol. 27, 80- 85
15 Ann, D. G. (1998) In Illustrated book of korean medicinal herbs. Kyohak Publishing Co., LTD., Seoul, pp. 598-599
16 Song, G. S., Ahn, B. Y., Lee, K. S., Maeng, L. K. and Choi, D. S. (1997) effect of hot water extracts from midicinal plants on the mutagenicity of indirect mutagens. Korean J. Food Sci. Technol. 29, 1288-1294
17 Baik, S. C., Kim, J. B., Cho, M. J., Kim, Y. C., Park, C. K., Ryou, H. H., Choi, H. J. and Rhee, K. H. (1990) Prevalence of H. pylori infection among normal korean adults (in Korean). J. Dorena SOC. Microbiol. 25, 455-462
18 Buege, J. A. and Aust, S. D. (1978) Microsomal lipid peroxidation. Method. Enzymol. 105, 302-310
19 Hertong, M. C. L., Feskens, E. J. M., Hoffman, P. C. H., Katan, M. B. and Kromhout, D. (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 342, 1007-1011   DOI   ScienceOn
20 Warren, J. R. and Marshall, B. J. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1, 1273-1275
21 Shetty, K. (2001) Biosynthesis and medical applications of Rosmarinic acid. J. Herbs spices & Medicinal Plants 8, 161- 181   DOI   ScienceOn
22 Kim, J. Y., Maeng, Y. S. and Lee, K. Y. (1995) Antioxidative effects of soybean extracts by using various solvents. Korean J. Food Sci. Technol. 27, 635-639