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Inhibitory Effect of Thapsigargin on the Activities of $H^+-ATPases$ in Tomato Roots  

Cho, Kwang-Hyun (Department of Agricultural Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Applied Biological Chemistry / v.48, no.3, 2005 , pp. 212-216 More about this Journal
Abstract
Thapsigargin is a specific antagonist of SR/ER-type $Ca^{2+}-ATPase$ in animal tissue, and it was used to characterize the microsomal ATPases prepared from the roots of tomato. When $10\;{\mu}M$ thapsigargin was added, it inhibited the microsomal ATPase activity by 30%. The thapsigargin-induced inhibition was dose-dependent. Since the activity of $Ca^{2+}-ATPase$ is very low in the roots of tomato tissue, it is possible that thapsigargin inhibits the activities of major $H^+-ATPases$ located in plasma and vacuolar membranes. The inhibitory effect of thapsigargin was reduced when the vacuolar $H^+-ATPase$ activity was inhibited by ${NO_3}^-$. However, the effect of thapsigargin was not observed on the $H^+-ATPase$ activity located in the plasma membrane. These results suggest that thapsigargin inhibits the vacuolar $H^+-ATPase$ activity in the roots of tomato.
Keywords
$H^+-ATPase$; Nitrate; Thapsigargin; Tomato roots; Vanadate;
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