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Biological Control of Perilla Culture by Burkhoderia sp. AK-17  

Kim, Keun-Ki (Department of Biotechnology, Miryang National University)
Kim, Yong-Kyun (Department of Biotechnology, Miryang National University)
Son, Hong-Joo (Department of Biotechnology, Miryang National University)
Choi, Young-Whan (Department of Horticulture, Miryang National University)
Kang, Kyu-Young (Division of Enviro-Biotechnology and Food Science and Technology, Gyeongsang National University)
Publication Information
Applied Biological Chemistry / v.48, no.1, 2005 , pp. 34-39 More about this Journal
Abstract
There are various crop diseases in green houses that are caused by the cultural environments, especially high temperature and moisture. To solve the forementioned problems, farmers are overusing agricultural chemicals, causing other damages by the chemical residue. In this study, antagonistic bacteria as biological control agents were isolated to produce the environmentally-friendly crops for use in green houses. Eighteen species of antagonistic bacteria were totally isolated from the soil and plants in the Perilla fields, and AK-17 showed the highest activity among the isolates. According to the results of anti-fungal spectrum against several pathogens by AK-17, the antagonism effect of the isolates was remarkable against grey mold rot by Botrytis cinerea, sclerotinia rot by Sclerotnia sclerotiorum, and stem rot by Rhizoctonia solini. To evaluate the biological control effects of the isolates against the major diseases of Perilla, studies were carried out to evaluate the preventive and the curative effects of the diseases throughout the pot experiments. According to the forementioned experiments, the preventive and the curative effects by the isolates against sclerotinia rot were respectively showed as 55% and 92%. For the grey mold rot, those were 40% and 78%, respectively. As to the evaluation of the growth-promoting effect by AK-17, the length and the biomass of the tested plants were increased to 120% and to 164%, respectively. For the leaf numbers and area were respectively increased to 120% and 220%. Furthermore, AK-17 was identified as Burkhoderia sp. according to the results of physiological properties and genetic methods.
Keywords
biological control; antagonistic bacteria; plant pathogens; plant growth promoting; Burkhoderia;
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1 Lee, K. M., Rhee, S. H., Park, K. Y. and Kim, J. O. (1992) Antimutagenic compounds identified from Perilla leaf. J. Korean Soc. Food Nutr. 21, 302-307
2 Lee, K. I., Rhee, S. H., Kim, J. O., Chung, H. Y. and Park, K. Y. (1993) Antimutagenic and antioxidative effects of Perilla leaf extracts. J. Korean Soc. Food Nutr. 22, 175-180
3 Kim, K. H., Chang, M. W., Park, K. Y., Rhee, S. H., Rhee, T. H. and Sunwoo, Y. I. (1993) Antitumor activity of phytol identified from perilla leaf and its augmentative effect on cellular immune response. Korean J. Nutr. 26, 379-389
4 Brenner, D. (1995) New crop homepage: Perilla. http://newcrop purdue.edu/hort/ newcrops/crops/cropFactSheets/perilla.html
5 Maclaren, D. L., Huang, H. C. and Rimmer, S. R. (1996) Control of apothecial production of Scletinia sclerotiorum by Coniothyrium minitans and Talaromyces flavus. Plant Dis. 80, 1373-1378   DOI   ScienceOn
6 Ann, S. D., Jang, B. H., Lee, M. S., Kwon, B. S. and Kim, N. N. (1996) In Survey of Economic Botany. Sunjin Munhwasa Press. Inc
7 Kim, K. K., Kang, J. G., Moon, S. S. and Kang, K. Y. (2000) Isolation and identification of antifungal N-butylbenzenesulphonamide produced by Pseudomonas sp. AB2. J. Antibiot. 53, 131-136
8 Lim, W. K., Park, S. G., Ryu, J. W., Sa, D. M., Lee, D. M. and Lim, G. O. (1996) In Economic Botany. Seo-il Press Inc. pp. 201-207
9 Palleroni, N. J. (1989) Family I. Pseudomonadaceae, In BERGEY's Manual Systemic Bacteriology. Volume 1. N. R. Krieg, (ed.) Williams & Wilkins, pp. 141-218
10 Shivanna, M. B., Meera, M. S. and Hyakumachi, M. (1996) Role of root colonization ability of plant growth promoting fungi in the suppression of take-all and common root rot of wheat. Crop Protect. 15, 497-504   DOI   ScienceOn
11 Siddiqui, Z. A. (2004) Effects of plant growth promoting bacteria and composed organic fertilizers on the reproduction of Meloidogyne incognita and tomato growth. Bioresource Technol. 95, 223-227   DOI   PUBMED   ScienceOn
12 Kim, C. S., Lee, J. P., Song, J. H., Lim, E. K., Chung, S. J., Ha, S. Y. and Moon, B. J. (2001) Development of biofungicide for control of gray mold rot of eggplant caused by Botrytis cinerea and bioassay in the greenhouse condition. Korean J. Life Sci. 11, 235-241
13 Chung, B. K. and Hong, K. S. (1991) Biological control with Streptomyces sp. on Fusarium oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica causing sesame wilt and blight. Kor. J. Mycol. 19, 231-237
14 Charudattan, R. and Dinoor, A. (2000) Biological control of weeds using plant pathogens: accomplishments and limitations. Crop Pro. 19, 691-695   DOI   ScienceOn
15 Shin, K. K., Yang, C. B. and Park, H. (1992) Studies on lipid and fatty acid composition of Korean perilla leaves (Penilla frutescens var. japonica HARA). Korean J. Food Sci. Technol. 24, 610-615
16 Jo, S. H., Park, T. H. and Chang, K. W. (2001) Effects of Brassiea campestris L. and Lactuca sativa L. yield by application of organic fertilizers and microorganisms. J. KOWREC. 9, 88-92
17 Lee, S. B. (2000) A view and development situation of microbial pesticide. Korean J. Pest. Sci. Pesticide News & Information, 4, 9-18
18 Kim, K. K., Park, K. H., Moon, S. S. and Kang, K. Y. (1997) Isolation and structure identification of antifungal substance from Aspergillus terreus. Agri. Chem. Biotechnol. 40, 593-596
19 Xiao, K., Kinkel, L. L. and Samac, D. A. (2002) Biological control of Phytophthora root rots on alfalfa and soybean with Streptomyces. Biol. Control. 23, 285-295   DOI   ScienceOn