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The Inhibitory Effect of Cornus walteri Extract Against ${\alpha}-amylase$  

Lim, Chae-Sung (Division of Biotechnology, Kangwon National University)
Li, Chun-Ying (Division of Biotechnology, Kangwon National University)
Kim, Yong-Mu (Division of Biotechnology, Kangwon National University)
Lee, Wi-Young (Korea Forest Research Institute)
Rhee, Hae-Ik (Division of Biotechnology, Kangwon National University)
Publication Information
Applied Biological Chemistry / v.48, no.1, 2005 , pp. 103-108 More about this Journal
Abstract
${\alpha}-Amylase$ inhibitor is used to control blood glucose level by inhibiting starch digestion in the small intestine and delaying the absorption of glucose. In this study, we investigated the effect of the ethanol extracts from more than 1400 species of plants against ${\alpha}-amylase$ with the aim of developing a new ${\alpha}-amylase$ inhibitor. In the results, Cornus walteri extracts showed the highest inhibition activity. The inhibitory effect of Cornus walteri extract on the carbohydrate hydrolysis enzymes has different sensitivities against ${\alpha}-amylase$ from salivary and pancreatin and against ${\alpha}-glucosidase$ from yeast and porcine small intestine. In the study of inhibition kinetics of ${\alpha}-amylase$ and ${\alpha}-glucosidase$, Cornus walteri extract showed competitive inhibition against salivary and pancreatin while showing the combination of uncompetitive and noncompetitive inhibition against ${\alpha}-glucosidase$. The Cornus walteri extract was stable at acidic and thermal conditions. As for the blood glucose and body weight levels of Cornus walteri extract, we confirmed anti-hyperglycemic and anti-obesity effects. Also, in the investigation of the mRNA lever, Cornus walteri extract upregulated the level of GLUT4 mRNA in the quadriceps muscle.
Keywords
${\alpha}-Amylase$ inhibitor; Cornus walteri; Anti-hyperglycemic effect; Anti-obesity effect;
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