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Purification of a New Elicitin from Phytopthora cambivora KACC40160  

Yoon, Sang-Hong (Metabolic Engineering Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Bae, Shin-Chul (Molecular Physiology Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Park, In-Cheol (Molecular Physiology Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Koo, Bon-Sung (Metabolic Engineering Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Kim, Young-Hwan (Metabolic Engineering Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Yeo, Yun-Soo (Metabolic Engineering Division, National Institute of Agricultural Biotechnology Rural Development Administration)
Publication Information
Applied Biological Chemistry / v.46, no.2, 2003 , pp. 79-83 More about this Journal
Abstract
Elicitins, proteinaceous elicitors secreted from Oomycetes fungi (Phytophthora spp. and Pythium spp.), have been known as inducer of hypersensitive response (HR) in incompatible interactions between plant and pathogens. Five elicitins among many Korean Phytophthora species caused the reactions of distal HR in radish, chinese cabbage and some hot pepper cultivars, but not in cucumber and tomato. Because the isolation of elicitin from Phytophthora cambivora hasn't been reported yet, we have purified a cambivorein, a new member of the elicitin family, from the culture filtrate of Phytophtilora cambivora (KACC 40160) by using FPLC (Fast Protein Liquid Chromatography, AKTA) with sepharose S and Sephacryl HR columns. We confirmed that it induces necrosis activities in some hot pepper cultivars and its molecular weight is about 10 KDa by Tricine-SDS-PAGE. Comparison of amino acid sequences of its N-terminal ends also informed the identification of Iysine at the 13th position, which is characteristic of a kind of basic elicitin isoform $({\beta}-isoform)$. It Also showed that our elicitin is not identical with N-terminal sequences of many elicitins reported from Phytophthora spp..
Keywords
elicitin; Phytophthora cambivora; hypersensitive response;
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