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Isolation and Identification of Terpenoids from the Flower of Rhododendron yedoense var. poukhanense  

Hong, Yoon-Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Song, Myoung-Chong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Han, Jae-Taek (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Jang, Tae-O (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Lee, Youn-Hyung (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Seung-Ae (Graduate School of East-West Medical Science, Kyung Hee University)
Park, Mi-Hyun (Eromlife Co. Ltd.)
Baek, Nam-Ln (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Applied Biological Chemistry / v.46, no.2, 2003 , pp. 144-149 More about this Journal
Abstract
Extracts were obtained from the flower of Rhododendro yedoense var. poukhanense. (7 kg) in 80% aqueous MeOH and successively fractionated with solvent of EtOAc, n-BuOH and $H_2O$, successively. Silica gel and ODS column chromatographies of the EtOAc and n-BuOH fractions were repeatedly carried out by using the various solvent systems to give five terpenoids. Chemical structures of the isolated terpenoids were determined as $2{\alpha},3{\beta}-dihydroxylolean-12-ene$ (1), ursolic acid (2), grayanotoxin IV (3), grayanotoxin I (4) and grayanotoxin III (5) based on the interpretation of several spectral data including 2D-NMR such as $^1H-^1H\;COSY$, HMQC and HMBC.
Keywords
Rhododendron yedonse var. poukhanense; dihydroxylolean-12-ene; ursolic acid; grayanotoxin;
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