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Isolation and Characterization of MMP-1 Inhibitor Peptide from Crataegus pinnatifida Bunge in Fibroblast Cell Line HS68 Cells  

Lee, Se-Young (Graduate School of Biotechnology, Korea University)
Chun, Hyug (Graduate School of Biotechnology, Korea University)
Cho, Hong-Yun (Graduate School of Biotechnology, Korea University)
An, Jeung-Hee (Graduate School of Biotechnology, Korea University)
Publication Information
Applied Biological Chemistry / v.46, no.1, 2003 , pp. 60-65 More about this Journal
Abstract
MMP-1 inhibitory compounds were isolated from 120 Korean traditional edible plants. UP- 1 activity significantly increased linearly with increasing UVB dose in normal human foreskin fibroblast HS68 cell, showing maximum activity at approximately 35 $mJ/cm^2$, whereas in HaCaT cell, normal human keratinocyte, no increase was observed. Maximum secretion of MMP-1 after UVB treatment occurred around 36-48 k after treatment. MMP-1 inhibitory compound isolated from cold-water fraction of Cataegus pinnatifida Bunge showed the mort potent activity. The MMP-1 inhibitory compound was deduced as a peptide based on the fact that pronase digestion decreased the activity whereas periodate oxidation did not. The most potent UP- 1-inhibitory protein, CP-2Va-2, showing an activity of 88.5% against MMP-1, was isolated through sequential column chromatography on DEAE-Toyopearl 650C, Butyl-Toyopearl 650M, and Bio-Gel P-30. Molecular weight of CP-2Va-2 determined through high performance liquid chromatography and SDS PACE was 19 and 20 kDa. respectively, signifying a monomeric structure.
Keywords
UVB; HS68 Cells; MMP-1 inhibitory activity; Crataegus pinnatifda Bunge; HS68 cell line;
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