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Antioxidative and Cytotoxicity Activities of Compounds Isolated from Korean Rhus verniciflua S.  

Choi, Won-Sik (Dept. of Life Science, Soonchunhyang University)
Kim, Dong-Kil (Dept. of Life Science, Soonchunhyang University)
Lee, Young-Haeng (Dept. of Chemistry, Won-Kwang University)
Kim, Jang-Eok (Dept. of Agricultural Chemistry, Kyungpook National University)
Lee, Sung-Eun (Dept. of Agricultural Chemistry, Kyungpook National University)
Publication Information
Applied Biological Chemistry / v.45, no.3, 2002 , pp. 168-172 More about this Journal
Abstract
Antioxidative activities of solvent fractions obtained from Korean Rhus vernicifera, being used in food industry and as a traditional medicine, were examined by the methods of DPPH scavenging activity, inhibitory effect on autooxidation of linoleic acid, nitrite scavenging activity and the inhibitory effect on xanthine oxidase activity. Ethyl acetate fraction showed potent antioxidative activities. Three compounds were isolated from the ethyl acetate fraction by rotatory locular counter current chromatography (RLCCC), Sephadex LH-20 column chromatography and HPLC. The isolated compounds were 1,2,3-trihydroxybezene, methyl 3,4,5-trihydroxybenzoate and 3,4,5-trihydroxybenzoic acid determined by GC/MS and, $^1H$ and $^{13}C$ NMR. Among the isolated compounds, methyl 3,4,5-trihydroxybenzoate showed the strongest antioxidative activity than artificial antioxidants, BHA and BHT in various methods. However, these isolated compounds did not show cytotoxicity effects on the human cancer cell lines. Therefore, we may suggest that methyl 3,4,5-trihydroxybenzoate can be used as a food additive possessing the potent antioxidative activity.
Keywords
Rhus verniciflua; methyl 3,4,5-trihydroxybenzoate; antioxidant; cytotoxicity;
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