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http://dx.doi.org/10.9718/JBER.2011.32.3.207

Automatic Switching System for The Impedance Analysis of Multichannel icroelectrode Arrays: Limits and Improvement Scheme  

Lee, Seok-Young (Department of Bio and Brain Engineering, KAIST)
Nam, Yoon-Key (Department of Bio and Brain Engineering, KAIST)
Publication Information
Journal of Biomedical Engineering Research / v.32, no.3, 2011 , pp. 207-217 More about this Journal
Abstract
Electrode impedances are measured to quantitatively characterize the electrode-electrolyte or cell-electrode interfaces. In the case of high-density microelectrode arrays(MEAs) that have been developed for brainmachine interface applications, the characterization process becomes a repeating and time-consuming task; a system that can perform the measurement and analysis in an automated fashion with accuracy and speed is required. However, due to the large number of channels, parasitic capacitance and off-capacitance components of the switching system become the major factors that decreased the accuracy for the measurement of high impedance microelectrodes. Here we investigated the implementation of automatic impedance measurement system with analyzing the causes of possible measurement-related problems in multichannel switching configuration. Based on our multi-channel measurement circuit model, we suggest solutions to the problems and introduce a novel impedance measurement scheme using electro-mechanical relays. The implemented measurement system could measure |Z| < 700 $k{\Omega}$ of impedance in - 10% errors, which can be widely applicable to high density neural recording MEAs.
Keywords
MEA; electrode impedance; automatic impedance measurement system; multichannel switching; electro-mechanical relay switch;
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1 D.A. Robinson, "The electrical properties of metal microelectrodes," Proceedings of the IEEE, vol. 56, no. 6, pp. 1065-1071, 1968.   DOI
2 C.H. Chen, D.J. Yao, S.H. Tseng, S.W. Lu, C.C. Chiao, and S.R. Yeh., "Micro-multi-probe electrode array to measure neural signals," Biosensors Bioelectron., vol. 24, no.7, pp. 1911-1917, 2009.   DOI   ScienceOn
3 D.T. Price, A.R.A. Rahman, S. Bhansali, "Design rule for optimization of microelectrodes used in electric cell-substrate impedance sensing(ECIS)," Biosensors Bioelectron., vol. 24, no. 7, pp. 2071-2076, 2009.   DOI   ScienceOn
4 Y. Nam, "Neuron-on-a-chip technology: Microelectrode array system and neuronal patterning," J. Biomed. Eng. Res., vol. 30, pp. 103-112, 2009.
5 R. Ehret, W. Baumann, M. Brischwein, A. Schwinde, K. Stegbause, and B. Wolf, "Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures," Biosensors Bioelectron., vol. 12, no. 1, pp. 29-41, 1997.   DOI   ScienceOn
6 D.R. Merrill and P.A. Tresco, "Impedance characterization of microarray recording electrodes in vitro," IEEE trans. Biomed. Eng., vol. 52, no. 11, pp. 1960-1965, 2005.   DOI   ScienceOn
7 G.T.A. Kovacs, "Microelectrode models for neural interfaces," in Enabling technologies for cultured neural networks, Academic Press, New York, 1994, pp. 121-165.
8 K. Gunalan, D.J. Warren, J.D. Perry, R.A. Normann, and G.A. Clark, "An automated system for measuring tip impedance and among-electrode shunting in high-electrode count microelectrode arrays," J. Neurosci. Methods, vol. 178, no. 2, pp. 263-269, 2009.   DOI   ScienceOn
9 Q. Liu, J. Yu, L. Xiao, J.C. Tang, Y. Zhang, P. Wang, and M. Yang, "Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays," Biosensors Bioelectron., vol. 24, no. 5, pp. 1305-1310, 2009.   DOI   ScienceOn
10 C.K. Liang, D.K. Chen, J.J. Chen, and S.C. Chen, "A multifunctional online measurement system for neuron-microelectrode interface study," in the 26th Annual International Conference of the IEEE Engineering In Medicine And Biology Society, San Francisco, CA, 2004.
11 D.A. Borkholder, "Cell based biosensors using microelectrodes," Doctoral thesis, Stanford university, 1998.
12 J. Pine, "Recording action potentials from cultured neurons with extracellular microcircuit electrodes," J. Neurosci. Methods, vol. 2, pp. 19-31, 1980.   DOI   ScienceOn
13 K.C. Cheung, "Implantable microscale neural interfaces," Biomed. Microdevices, vol. 9, no. 6, pp. 923-938, 2007.   DOI   ScienceOn
14 C.A.J. Thomas, P.A. Springer, G.E. Loeb, Y. Berwald-Netter, and L.M. Okun, "A miniature microelectrode array to monitor the bioelectric activity of cultured cells," Exp. Cel. Res., vol. 74, pp. 61-66, 1972.   DOI   ScienceOn
15 G.W. Gross, "Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface," IEEE Trans. Biomed. Eng., vol. 26, pp. 273-279, 1979.