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http://dx.doi.org/10.9718/JBER.2020.41.2.67

Analysis for Concentration Range of Fluorescein Sodium  

Lee, Da-Ae (Department Nano-physics, College of Bionano Technology, Gachon University)
Kim, Yong-Jae (Department Bio-nano Science, College of Bionano Technology, Gachon University)
Yoon, Ki-Cheol (Department of Biomedical Engineering, College of Medicine, Gachon University)
Kim, Kwang-Gi (Department of Biomedical Engineering, College of Medicine, Gachon University)
Publication Information
Journal of Biomedical Engineering Research / v.41, no.2, 2020 , pp. 67-74 More about this Journal
Abstract
Brain tumors or gliomas are fatal cancer species with high recurrence rates due to their strong invasiveness. Therefore, the goal of surgery is complete tumor resection. However, the surgery is difficult to distinguish the border because tumors and blood vessels have the same color tone and shape. The fluorescein sodium is used as a fluorescence contrast agent for boundary separation. When the external light source is irradiated, yellow fluorescence is expressed in the tumor, which helps distinguish between blood vessels and tumor boundaries. But, the fluorescence expression of fluorescence sodium depends on the concentration of fluorescein sodium and such analytical data is insufficient. The unclear fluorescence can obscure the boundaries between blood vessels and tumors. In addition, reduce the efficiency of fluorescence sodium use. This paper proposes a protocol of concentration range for fluorescence expression conditions. Fluorescent expression was observed using a near-infrared (NIR) color camera with corresponding dilution using normal saline in 1 ml microtube. The flunoresence emission density range is 1.00 mM to 0.15 mM. The fluorescence emission begin to 1.00 mM and the 0.15 mM discolor. The discolor is difficult to fluorescence emission condition obserbation. Thus, the maximum density range of the bright fluoresecein is 0.15 mM to 0.30 mM. When the concentration range of fluorescein sodium is analyzed based on the gradient of fluorescence expression and the power measurement, the brightest fluorescence is expected to facilitate the complete resection of the tumor. For the concentration range protocol, setting concentration ranges and analyzing fluorescence expression image according to saturation and brightness to find optimal fluorescence concentration are important. Concentration range protocols for fluorescence expression conditions can be used to find optimal concentrations of substances whose expression pattern varies with concentration ranges. This study is expected to be helpful in the boundary classification and resection of brain tumors and glioma.
Keywords
Fluorescein sodium; NIR camera; Dilution; Half-life-time; Normal saline;
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1 Schebesch KM, Brawanski A and Hohne J. Fluorescein sodium-guided surgery of maliganant brain tumors:history, current concepts, and future project. Turkish Neuorsurgery. 2016;26(2):185-94.
2 Schebesch KM, Proescholdt M, HOhne J, Hohenberger C, Hansen E, Riemenschneider MJ, Ullrich W, Doenitz C, Schlaier J, Lange M and Brawanski A. Sodium fluorescein-guided resection under the YELLOW 560 nm surgical microscope filter in malignant brain tumor surgery-a feasibility study. Acta Neurochir. 2013;155:693-9.   DOI
3 Belykh EG, Zhao X, Cavallo C, Bohl MA, Yamurlu K, Aklinski JL, Byvaltsev VA, Sanai N, Spetzler RF, Lawton MT, Nakaji P and Preul MC. Laboratory evaluation of a robotic operative microscope-visualization platform for neurosurgery. Cureus. 2018;10(7):2-22.   DOI
4 Setten GV. Sandbank Epitheliopathy of the conjunctiva (SEC) a new indicator in dry eye. J. Eye & Cataract Surgery. 2017;3(2):1-4.   DOI
5 Fluorescite Ophthalmic Disclosing Agent Fluorescein Sodium (https://allmedtech.com/flbopdiagfls.html).
6 Shingnapurkar SH, Mitra DK, Kadav MS, Shah RA, Rodrigues SV and Prithyani SS. The effect of indocyanine green-mediated photodynamic therapy as an adjunct to scaling and root planing in the treatment of chronic periodontitis: A comparative splitmouth randomized clinical trial. Indian J. Dental Researh. 2016;27(6):609-17.   DOI
7 Bennett TJ, Quillen DN and Coronica R. Fundamentals of fluorescein angiography. American Soc. Ophthalmic Registered Nurses Insight. 2016;41(1):5-11.
8 Lee HM, Kim HR, Yoon WB, Kim YJ, Kim KG, Kim SK, Yoo H, Lee SH, Shin M S and Kwon KC. Study of a brain tumor and blood vessel detection system using multiple fluorescence imaging by a surgical microscope. Korean J. Opt. Photon. 2015;26(1):23-9.   DOI
9 Cherrick, G. R, Stein, S. W, Leevy, C. M, & Davidson, C. S. Indocyanine green: observations on its physical properties, plasma decay, and hepatic extraction. J Clin Invest. 1960;39(4):592-600.   DOI
10 DR W. Fluorescein angiography basic science and engineering. Ophthalmology. 1986;93(2):1617-20.   DOI
11 Schebesch KM, Proescholdt M, Hohne J, Hohenberger C, Hansen E, Riemenschneider MJ, Ullrich W, Doenits C, Schlaier J, Lange M and Brawanski A. Sodium fluorescein-guided resection under the yellow 560 nm surgical microscope filter in malignant brain tumor surgery-a feasibility study. Acta Neurochirurgica. 2013;155(4):693-9.   DOI
12 Suzuki K, Kodama N, Sasaki T, Matsumoto M, Ichikawa T, Munakata R, Muramatsu H and Kasuya H. Confirmation of blood flow in perforating arteries using fluorescein cerebral angiography during aneurysm surgery. J. Neurosurgery. 2007;107:68-73.   DOI
13 Chen B, Wang H, Ge P, Zhao J, Li W, Gu H, Wang G, Luo Y and Chen D. Gross total resection of glioma with the intraoperative fluorescence-guidance of fluorescein sodium. Int. J. Medical Sciences. 2012;9(8):708-14.   DOI
14 Lefranc F, Sadeghi N, Camby I, Metens T, Dewitte O and Kiss R. Present and potential future issues in glioblastoma treatment. J. Expert Review of Anticancer Therapy. 2006; 6(5):719-32.   DOI
15 Christopoulos TK and Diamandis EP. Fluorescence immunoassays. Hum Pathol. 1984;15(2):112-6.   DOI
16 Eforn, N. Contact lens complications 3rd. Elsevier Expert Consult, USA:Saunders;2004.
17 Polomska AK, Lerous JC and Detmar M. Minimally invasive method for the point-of-care quantification of lymphatic vessel function. JCI Insight. 2019;4(4):e126515.   DOI
18 Kim TW, Park JH and Hong JI. Self-quenching mechanism: the influence of quencher and spacer on quencher-fluorescein probes. Bull. Korean Chem. Soc. 2007;28(7):1221-3.   DOI
19 web site on www.cancer.go.kr (definition of brain gliomas)
20 Larcroix M, Abi-Said D, Fourney DR, Gokaslan ZL, Shi W, DeMonte F, Lang FF, McCutcheon IE, Hassenbusch SJ, Holland E, Hess K, Michael C, Miller D and Sawaya R. A multivariate analysis of 416 patients with glioblastoma multiforme: prognosis, extent of resection, and survival. J. Neurosurgery. 2001;95(2):190-8.   DOI
21 Stummer W, Novotny A, Stepp H, Goetz C, Bise K and Reulen HJ. Fluorescence-guided resection of glioblastoma multiforme utilizing 5-ALA-induced porphyrins: a prospective study in 52 consecutive patients. J. Neurosurgery. 2000; 93(6):1003-13.   DOI
22 Shinoda J, Yano H, Yoshimura SI, Okumura A, Kaku Y, Iwama T and Sakai N. Fluorescence-guided resection of glioblastoma multiforme by using high-dose fluorescein sodium. J. Neurosurgery. 2003;99:597-603.   DOI
23 Flower RW. Injection technique for indocyanine green and sodium fluorescein dye angiography of the eye. Investigative Ophthalmology & Visual Science. 1973;12(12):881-95.
24 Mondal SB, Gao S, Zhu N, Liang R, Gruev V and Achilefu S. Real-time fluorescence image-guided oncologic surgery. Adv. Cancer Research. 2014;124:171-211.   DOI
25 Spaide RF, Klancnik JM and Cooney J. Retinal vascular layers Imaged by fluorescein angiography and optical coherence tomography angiography. JAMA Ophthalmology. 2015;133(21):45-50.   DOI
26 Keerl R, Weber RK, Draf W, Wienke A, and Schaefer SD. Use of sodium fluorescein solution for detection of cerebrospinal fluid fistulas: An analysis of 420 administrations and reported complications in Europe and the United states. Laryngoscope. 2004;114(2):266-72.   DOI
27 Koc K, Anik I, Cabuk B And Ceylan S. Fluorescein sodiumguided surgery in glioblastoma multiforme: a prospective evaluation. British J. Neurosurgery. 2008;22(1):99-103.   DOI