Browse > Article
http://dx.doi.org/10.9718/JBER.2020.41.1.1

Effect of PVA Concentration on Strength and Cell Growth Behavior of PVA/gelatin Hydrogels for Wound Dressing  

Kim, Soyeun (Department of Biomedical Engineering, Daelim University)
Lim, Hyunju (Department of Biomedical Engineering, Daelim University)
Kim, Sojeong (Department of Biomedical Engineering, Daelim University)
Lee, Deuk Yong (Department of Biomedical Engineering, Daelim University)
Publication Information
Journal of Biomedical Engineering Research / v.41, no.1, 2020 , pp. 1-7 More about this Journal
Abstract
Polyvinyl alcohol (PVA)/gelatin hydrogels were prepared by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the strength and the cell growth behavior of the PVA/gelatin hydrogels. The swelling rate of the PVA/gelatin hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel. No appreciable degradation of the hydrogels was detected. As the PVA content increased from 6 wt% to 12 wt%, the strength of the PVA/gelatin hydrogels increased drastically from 6.4±0.9 kPa to 46.6±9.0 kPa. The PVA/gelatin hydrogels did not show any evidence of causing cell lysis or toxicity, implying that the hydrogels are clinically safe and effective. Although the strength increased with increasing the PVA content, the PVA/gelatin hydrogels containing 8 wt% exhibited the fastest cell growth, which is highly suitable for wound dressing requiring fast healing regeneration.
Keywords
Polyvinyl alcohol (PVA); Gelatin; Hydrogel; Freezing/thawing; Cytotoxicity; Cell proliferation and growth;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Lim KS, Alves MH, Poole-Warren LA, and Martens PJ. Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels. Biomater. 2013;34(29):7097-105.   DOI
2 Wang F, Guo E, Song E, Zhao P, and Liu J. Structure and properties of bone-like-nanohydroxyapatite/gelatin/polyvinyl alcohol composites. Adv. Biosci. Biotechnol. 2010; 1(3):185-9.   DOI
3 Mahnama H, Dadbin S, Frounchi M, and Rajabi S. Preparation of biodegradable gelatin/PVA porous scaffolds for skin regeneration. Artif. Cells Nanomed. Biotechnol. 2017;45(5):928-35.   DOI
4 Kim J, Lee DY, Kim E, Jang J, and Cho N. Tissue response to implant of hyaluronic acid hydrogel prepared by microbeads. Tiss. Eng. Regen. Med. 2014;11(1):32-8.   DOI
5 Lee DY, Cheon C, Son S, Kim Y, Kim J, Jang J, and Kim S. Influence of molecular weight on swelling and elastic modulus of hyaluronic acid dermal fillers. Polym. Korea. 2015;39(6):976-80.   DOI
6 Chun C, Lee DY, Kim Y, Kwon M, Kim Y, and Kim S. Effect of molecular weight of hyaluronic acid on viscoelastic and particle texturing feel properties of HA dermal biphasic fillers. Biomater. Res. 2016;20(1):275281.
7 Seol B, Shin J, Oh G, Lee DY, and Lee M. Characteristics of PU/PEG hybrid scaffolds prepared by electrospinning. J. Biomed. Eng. Res. 2017;38:248-55.   DOI
8 Oh G, Rho J, Lee DY, Lee M, and Kim Y. Synthesis and characterization of electrospun PU/PCL hybrid scaffolds. Macromol. Res. 2018;26(1):48-53.   DOI
9 Kim Y, Son S, Chun C, Kim J, Lee DY, Choi HJ, and Kim T. Effect of PEG addition on pore morphology and biocompatibility of PLLA scaffolds prepared by freeze drying. Biomed. Eng. Lett. 2016;6(15):287-95.   DOI
10 Torelli GF, Rozera C, Santodonato L, Peragine N, D'Agostino G, Montefiore E, Napolitano MR, Monque DM, Carlei D, Mariglia P, Pauselli S, Gozzer M, Bafti MS, Girelli G, Guarini A, Belardelli F, and Foa R. A good manufacturing practice method to ex vivo expand natural killer cells for clinical use. Blood Tansfus. 2015;13(3):464-71.
11 Cho D and Campana D. Expansion and activation of natural killer cells for cancer immunotherapy. Korean J. Lab. Med. 2009;29(2):89-96.   DOI
12 Santos SC, Custodio CA, and Mano JF. Photopolymerizable platelet lysate hydrogels for customizable 3D cell culture platforms. Adv. Healthcare Mater. 2018:1800849.   DOI
13 Jo S, Lim Y, Youn M, Gwon H, Park J, Nho Y, and Shin H. Fabrication and characterization of PVA/CMC hydrogels by freezing-thawing technique and gamma-ray irradiation. Polym. Korea. 2009;33(6):551-4.
14 Han S and You H. Wound coverage using advanced technology in Korea. J. Korean Med. Assoc. 2011;54(6):594-603.   DOI
15 Kim J, Lee CM, Kim D, and Lee K. Development of aloin loaded PVA/CMC hydrogel for wound healing. Polym. Korea. 2013;37(6):802-8.   DOI
16 Hassan CM and Peppas NA. Structure and morphology of freeze/thawed PVA hydrogels. Macromolecules. 2000;33(7):2472-9.   DOI
17 Shin J, Jeong H, and Lee DY. Synthesis and biocompatibility of PVA/NaCMC hydrogels crosslinked by cyclic freezing/thawing and subsequent gamma-ray irradiation. J. Biomed. Eng. Res. 2018;39(4):161-7.   DOI
18 Hwang S, Ahn S, Park J, Jeong SI, Gwon H, Lee DY, and Lim Y. Characterization and preparation of the hydrogel has excellent release effect of the active ingredients using a radiation crosslinking technology. J. Radiat. Industry. 2015;9(2):199-207.