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http://dx.doi.org/10.9718/JBER.2018.39.6.250

A novel Method for Blood Typing using Acoustic Streaming  

Choi, Hyunjoo (Department of Medical Sciences, Graduate School of Medicine, Korea University)
Jang, Woong Sik (Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital)
Nam, Jeonghun (Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital)
Lim, Chae Seung (Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital)
Publication Information
Journal of Biomedical Engineering Research / v.39, no.6, 2018 , pp. 250-255 More about this Journal
Abstract
Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.
Keywords
Lamb wave; Blood analysis; Blood typing; Agglutination;
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1 In Bum Suh, Sook Won Ryu, Yongku Lee, Dae Sung Hr, Chanil Chung, Jun Keun Chang and Chae Seug Lim, "Development of a new blood typing kit using the microfluidics separation technique," Korean J Hematol. vol. 42, no. 4, 2007.
2 T. Kline, M. Runyon, M. Pothiawala and R. Ismagilov, "ABO, D Blood Typing and Subtyping Using Plug-Based Microfluidics," Analytical Chemistry, vol. 80, no. 16, pp. 6190-6197, 2008.   DOI
3 A. Nilghaz, D. Ballerini, L. Guan, L. Li and W. Shen, "Red blood cell transport mechanisms in polyester thread-based blood typing devices," Analytical and Bioanalytical Chemistry, vol. 408, no. 5, pp. 1365-1371, 2015.
4 H. Ashiba, M. Fujimaki, K. Awazu, T. Tanaka and M. Makishima, "Microfluidic chips for forward blood typing performed with a multichannel waveguide-mode sensor," Sensing and Bio-Sensing Research, vol. 7, pp. 121-126, 2016.   DOI
5 T. Songjaroen and W. Laiwattanapaisal, "Simultaneous forward and reverse ABO blood group typing using a paper-based device and barcode-like interpretation," Analytica Chimica Acta, vol. 921, pp. 67-76, 2016.   DOI
6 Amgad R. Rezk and Leslie Y. Yeo, "Lithography-free, crystal-based multiresonant Lamb waves for reconfigurable microparticle manipulation," Journal of Microelectronics, Electronic Components and Materials, vol. 46, no. 4, pp. 176-182, 2016.
7 N. Yeow, H. McLiesh, L. Guan, W. Shen and G. Garnier, "Paper-based assay for red blood cell antigen typing by the indirect antiglobulin test" Analytical and Bioanalytical Chemistry, vol. 408, no. 19, pp. 5231-5238, 2016.   DOI
8 H. Zhang, X. Qiu, Y. Zou, Y. Ye, C. Qi, L. Zou, X. Yang, K. Yang, Y. Zhu, Y. Yang, Y. Zhou and Y. Luo, "A dye-assisted paper-based point-of-care assay for fast and reliable blood grouping," Science Translational Medicine, vol. 9, no. 381, pp. eaaf9209, 2017.   DOI
9 A. Rezk, J. Friend and L. Yeo, "Simple, low cost MHz-order acoustomicrofluidics using aluminium foil electrodes," Lab Chip, vol. 14, no. 11, pp. 1802-1805, 2014.   DOI
10 G. Destgeer, B. Ha, J. Park and H. Sung, "Lamb Wave-Based Acoustic Radiation Force-Driven Particle Ring Formation Inside a Sessile Droplet," Analytical Chemistry, vol. 88, no. 7, pp. 3976-3981, 2016.   DOI
11 R. Shilton, M. Tan, L. Yeo and J. Friend, "Particle concentration and mixing in microdrops driven by focused surface acoustic waves," Journal of Applied Physics, vol. 104, no. 1, pp. 014910, 2008.   DOI
12 Leslie Y. Yeo and James R. Friend, "Surface Acoustic Wave Microfluidics," The Annual Review of Fluid Mechanics, vol. 46, pp. 379-406, 2013.
13 L. Yeo and J. Friend, "Ultrafast microfluidics using surface acoustic waves," Biomicrofluidics, vol. 3, no. 1, pp. 012002, 2009.   DOI
14 J. Nam, H. Choi, J. Kim, W. Jang and C. Lim, "Lamb wave-based blood coagulation test," Sensors and Actuators B: Chemical, vol. 263, pp. 190-195, 2018.   DOI