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http://dx.doi.org/10.9718/JBER.2017.38.6.308

Effect and Response of Skeletal Muscle Cells on Electrical Stimulation Condition  

Seo, Hyung Woo (Dept. of Biomedical Engineering, Konyang University)
Shin, Hyun Young (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Lee, Hyun-Ju (Dept. of Physical Therapy, Konyang University)
Tae, Ki-Sik (Dept. of Biomedical Engineering, Konyang University)
Kim, Minseok S. (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Publication Information
Journal of Biomedical Engineering Research / v.38, no.6, 2017 , pp. 308-312 More about this Journal
Abstract
Skeletal muscle function plays a very important role in quality of life. However, skeletal muscle causes functional decline under aging or some diseases. Exercise and muscle training are good solutions to delay sarcopenia, but there are limitations to those who are uncomfortable in exercise. For this reason, alternative interventions for muscle sarcopenia are required, and many studies proved the increase of skeletal muscle mass by electrical stimulation. In conventional studies, however, mouse skeletal muscle cells have been mostly used in experiments to identify electrical stimulation conditions while human derived cells have not been frequently utilized in these studies. Stimulation used for rehabilitation has been uniformly treated without the consideration of aging. In addition, many studies have been used with conventional petri dish usually requiring many numbers of cells, which is not appropriate for rare. Moreover, they are not usually condition uniformity of electrical field. In this study, we have developed an electrical stimulation device which consumes a small amount of cells and can form a uniform electrical field. With the system, we analyzed the skeletal muscle differentiation and Myotube thickness depending on the electrical stimulation condition.
Keywords
Skeletal muscle cell; Electrical stimulation; Cell viability;
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1 C. Cheng, B. Davis, L. Madden, N. Bursac and G. Truskey, "Physiology and metabolism of tissue-engineered skeletal muscle", Experimental Biology and Medicine, vol. 239, no. 9, pp. 1203-1214, 2014.   DOI
2 G. Cittadella Vigodarzere and S. Mantero, "Skeletal muscle tissue engineering: strategies for volumetric constructs", Frontiers in Physiology, vol. 5, 2014.
3 A. Petersen, "The anti-inflammatory effect of exercise", Journal of Applied Physiology, vol. 98, no. 4, pp. 1154-1162, 2005.   DOI
4 N. Jones and K. Killian, "Exercise Limitation in Health and Disease", New England Journal of Medicine, vol. 343, no. 9, pp. 632-641, 2000.   DOI
5 Y. Liu, R. Grumbles and C. Thomas, "Electrical Stimulation of Embryonic Neurons for 1 Hour Improves Axon Regeneration and the Number of Reinnervated Muscles That Function", Journal of Neuropathology & Experimental Neurology, vol. 72, no. 7, pp. 697-707, 2013.   DOI
6 A. Ito, Y. Yamamoto, M. Sato, K. Ikeda, M. Yamamoto, H. Fujita, E. Nagamori, Y. Kawabe and M. Kamihira, "Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation", Scientific Reports, vol. 4, no. 1, 2014.
7 K. Ikeda, A. Ito, M. Sato, Y. Kawabe and M. Kamihira, "Improved contractile force generation of tissue-engineered skeletal muscle constructs by IGF-I and Bcl-2 gene transfer with electrical pulse stimulation", Regenerative Therapy, vol. 3, pp. 38-44, 2016.   DOI
8 B. Zhang, S. Yeung, Y. Liu, H. Wang, Y. Wan, S. Ling, H. Zhang, Y. Li and E. Yeung, "The effects of low frequency electrical stimulation on satellite cell activity in rat skeletal muscle during hindlimb suspension", BMC Cell Biology, vol. 11, no. 1, p. 87, 2010.   DOI
9 A. Ito, Y. Yamamoto, M. Sato, K. Ikeda, M. Yamamoto, H. Fujita, E. Nagamori, Y. Kawabe and M. Kamihira, "Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation", Scientific Reports, vol. 4, no. 1, 2014
10 H. Kern, L. Barberi, S. Lofler, S. Sbardella, S. Burggraf, H. Fruhmann, U. Carraro, S. Mosole, N. Sarabon, M. Vogelauer, W. Mayr, M. Krenn, J. Cvecka, V. Romanello, L. Pietrangelo, F. Protasi, M. Sandri, S. Zampieri and A. Musaro, "Electrical Stimulation Counteracts Muscle Decline in Seniors", Frontiers in Aging Neuroscience, vol. 6, 2014.
11 M. Langelaan, K. Boonen, K. Rosaria-Chak, D. van der Schaft, M. Post and F. Baaijens, "Advanced maturation by electrical stimulation: Differences in response between C2C12 and primary muscle progenitor cells", Journal of Tissue Engineering and Regenerative Medicine, vol. 5, no. 7, pp. 529-539, 2010.   DOI
12 N. Nikoli , S. Skaret Bakke, E. Tranheim Kase, I. Rudberg, I. Flo Halle, A. Rustan, G. Thoresen and V. Aas, "Electrical Pulse Stimulation of Cultured Human Skeletal Muscle Cells as an In Vitro Model of Exercise", PLoS ONE, vol. 7, no. 3, p. e33203, 2012.   DOI
13 I. Evers-van Gogh, S. Alex, R. Stienstra, A. Brenkman, S. Kersten and E. Kalkhoven, "Electric Pulse Stimulation of Myotubes as an In Vitro Exercise Model: Cell-Mediated and Non-Cell-Mediated Effects", Scientific Reports, vol. 5, no. 1, 2015.
14 Y. Kawahara, K. Yamaoka, M. Iwata, M. Fujimura, T. Kajiume, T. Magaki, M. Takeda, T. Ide, K. Kataoka, M. Asashima and L. Yuge, "Novel Electrical Stimulation Sets the Cultured Myoblast Contractile Function to 'On' ", Pathobiology, vol. 73, no. 6, pp. 288-294, 2006.   DOI