Browse > Article
http://dx.doi.org/10.9718/JBER.2017.38.4.175

The Effect of Microcurrent Electrical Stimulation on Muscle Atrophy Suppression in a Sciatic Nerve Injured Rat Model; Comparative Study by Current Intensity  

Hwang, Donghyun (Department of Biomedical Engineering, Yonsei University)
Kim, Seohyun (Department of Biomedical Engineering, Yonsei University)
Lee, Hana (Department of Biomedical Engineering, Yonsei University)
Jang, Seungjun (Department of Biomedical Engineering, Yonsei University)
Kim, Sebin (Department of Biomedical Engineering, Yonsei University)
kim, Tackjoong (Division of biological science and technology)
Choi, Sooim (YD Life science company)
Kwak, Hoyoung (YD Life science company)
Kim, Han Sung (Department of Biomedical Engineering, Yonsei University)
Publication Information
Journal of Biomedical Engineering Research / v.38, no.4, 2017 , pp. 175-182 More about this Journal
Abstract
Microcurrent electrical stimulation(MES) has been used to accelerate recovery of atrophied skeletal muscle. However, convincing stimulation parameters for suppressing muscle atrophy due to injured sciatic nerve remains unclear. The objective of this study was to investigate the effective intensity of MES on restraining muscle atrophy with rat model underwent sciatic nerve injury(SNI). Twenty-5-week-old Sprague Dawley male rats were equally assigned to five groups : Control group(Control, CON, n = 4), Denervation group(Denervation, D, n = 4), Denervation with MES of $22{\mu}A$ group(Denervation + $22{\mu}A$, D+22, n = 4), Denervation with MES of $100{\mu}A$ group (Denervation + $100{\mu}A$, D+100 n = 4), Denervation with MES of $400{\mu}A$ group(Denervation + $400{\mu}A$, D+400, n = 4). To induce muscle atrophy, all rats in the D, D+22, D+100, and D+400 groups, were subjected to sciatic nerve injury on their right hindlimb and allowed to have 1 week of resting period. Following this period, rats underwent daily MES(60 min/ a day, 5times/1week) for 4 weeks. After that, we investigate morphological changes in muscle volume by using in vivo micro-computed tomography at week 0, 1, 3 and 5. After 5 weeks, the muscle volume had the highest value in D+400 group, and also noticeably increased in D+100 group compared to it in D group. The results of this study imply that MES with current intensities between $100-400{\mu}A$ can suppress muscle atrophy effectively.
Keywords
Muscle atrophy; Micro-current therapy; Sciatic nerve injury; Micro-CT;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Kang, Da-Haeng, Jae-Keun Jeon, and Joon-Hee Lee, "Effects of low-frequency electrical stimulation on cumulative fatigue and muscle tone of the erector spinae," Journal of physical therapy science, vol. 27, no. 1, pp. 105-108, 2015.   DOI
2 W.T. Lim, N.G. Lee, and B.S. Choi, "Disuse Atrophy of Skeletal Muscle : From Bench to Bedside," Korea Journal of Neural Rehabilitation, vol. 6, no. 1, pp. 50-55, 2016.
3 Y.J. Jung, "Effects of hemiplegia and pamplegia induced by sciatic nerve injury on both tibiae and lumbar vertebra trabecular bone; Correlation between the magnitude and Rate of Bone loss and baseline bone Quantity(BV/TV)(Masters dissertation)," Graduate School, Yonsei University, Korea, 2013.
4 Robert, W. J., and Susan, C. K., "The molecular basis of skeletal muscle atrophy," American Journal of Physiology-Cell Physiology, vol. 287, no. 4, pp. 834-843, 2004.   DOI
5 G.H. Won, C. Kim, and C.G. Kim, "Effects of Electromyostimulation and Weight Training on Muscle Morphology and Function," The Korea Journal of Education, vol. 40, no. 1, pp. 490-498, 2001.
6 G.M. Eom, G. Khang, and J.H. Yi, "Restoration of Motor Function using Electrical Stimulation : Functional Electrical Stimulation (FES)," Journal of the Korean Society of Precision Engineering, vol. 20, no. 1, pp. 26-35, 2003.
7 Y.J. Jung, S.J. Ko, H.M. Yoo, and D.Y. Jung, "Effects of Transcutaneous Electrical Nerve Stimulation and Microcurrent Electrical Neuromuscular Stimulation on Delayed Onset Muscle Soreness," Korean Research Society of Physical Therapy, vol. 7, no. 2, pp. 76-87, 2000.
8 D.J. Lee, D.Y. Lee, and D.Y. Hwang, "Effects of Ultrasound and High-Voltage Pulsed Current on Adjuvant-Induced Arthritis in Rats," Korean Research Society of Physical Therapy, vol. 13, no. 3, pp. 33-40, 2006.
9 Mercola, J. M., and Kirsch, D. L., "The basis for microcurrent electrical therapy in conventional medical practice," Journal of Advancement in medicine, vol. 8, no. 2, pp. 107-120, 1995.
10 H.U. Moon, "Effects of Microcurrent and High Voltage Pulsed Galvanic Current Stimulation on Fibular Fracture Healing of the Rabbits," The Korea Contents Association, vol. 11, no. 10, pp. 287-292, 2011.   DOI
11 W.A. Kwon, R.J. Park, Y.K. Park, and T.Y. Hwang, "The Effects of Pulsed Electromagnetic Energy and Microcurrent on wound Healing in Rabbits," The Korean Society of Physical Therapy, vol. 12, no. 3, pp. 319-329, 2000.
12 Lambert, M. I., Marcus, P., Burgess, T., and Noakes, T. D., "Electro-membrane microcurrent therapy reduces signs and symptoms of muscle damage," Medicine and Science in Sports and Exercise, vol. 34, no. 4, pp. 602-607, 2002.   DOI
13 R.J. Park, S.J. Choi, G.A. Cheng, M.S. Cho, J.S. Cho, Y.M. Lee, Y.H. Cho, and S.H. Park, "Effects of Induced Microcurrent Shoes on Fatigue and Pain in Painful Foot to Patients with Plantar Fascitis," The Korean Society of Physical Therapy, vol. 18, no. 1, pp. 1-10, 2006.
14 T.Y. Kim, E.Y. Choi, and H.J. Yoon, "The Effects of Microcurrent Electrical Neuromuscular Stimulation on Delayed Onset Muscle Soreness, Serum Creatine Kinase, and Maximal Voluntary Isometric Contraction: A Preliminary Report," The journal of Korean academy of physical therapist, vol. 2, no. 3, pp. 587-598, 1995.
15 J.W. Jung, "A Study for Pain Relief Effect of Microcurrent," The journal of Korean academy of physical therapist, vol. 12, no. 2 pp. 195-205, 1991.
16 H.J. Oh, J.Y. Kim, and R.J. Park, "The Effects of Microcurrent Stimulation on Recovery of Function and Pain in Chronic Low Back Pain," Korean Society of Physical Medicine, vol. 3, no. 1, pp. 47-56, 2008.
17 Bayat, M., Asgari-Moghadam, Z., Maroufi, M., & Rezaie, F. S., "Experimental wound healing using microamperage electrical stimulation in rabbits," Journal of rehabilitation research and development, vol. 43, no. 2, pp. 219-226, 2006.   DOI
18 S.D. Kim, H.M. Park, and H.S. Jung, "The Comparison of Effect of MC Intensity in Pain and ROM in Delayed Onset Muscle Soreness," Journal of the Korean Academy of Clinical Electrophysiology, vol. 7, no. 1, pp. 1-6, 2009.   DOI
19 Chang-Yong Ko, Dong-Hyun Seo, and Han Sung Kim, "Suggestion of methodology for evaluation of abdominal adipose tissue of C57BL/6 female mice using in-vivo Micro-CT," Tissue Engineering and Regenerative Medicine, vol. 7, no. 4, pp. 410-418, 2010.
20 Yoshitaka Ohno, Hiroto Fujiya, Ayumi Goto, Ayane Nakamura, Yuka Nishiura, Takao Sugiura, Yoshinobu Ohira, Toshitada Yoshioka, Katsumasa Goto, "Microcurrent electrical nerve stimulation facilitates regrowth of mouse soleus muscle," Int J Med Sci, vol. 10, no. 10, pp. 1286-1294, 2013.   DOI
21 Fujiya, H., Ogura, Y., Ohno, Y., Goto, A., Nakamura, A., Ohashi, K., Uematsu, D., Aoki, H., Musha, H. & Goto, K., "Microcurrent electrical neuromuscular stimulation facilitates regeneration of injured skeletal muscle in mice," Journal of sports science & medicine, vol. 14, no. 2, pp. 297, 2015.
22 Yoshida, A., Fujiya, H., Goto, K., Kurosaka, M., Ogura, Y., Yatabe, K., Yoshioka, H., Terauchi, K., Funabashi, T., Akema, T., Niki, H. and Musha, H., "Regeneration of injured tibialis anterior muscle in mice in response to microcurrent electrical neuromuscular stimulation with or without icing," Journal of St. Marianna University, vol. 6, no. 2, pp. 159-169, 2015.   DOI
23 Zickri, Maha Baligh, "Possible Local Stem Cells Activation by Microcurrent Application in Experimentally Injured Soleus Muscle," International journal of stem cells, vol. 7, no. 2, pp. 79-86, 2014.   DOI
24 Fan, Yongjun, Kathleen G. Dickman, and Wei-Xing Zong, "Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition," Journal of Biological Chemistry, vol. 285, no. 10, pp. 7324-7333, 2010.   DOI
25 Takao Sugiura, Noritaka Abe, Mai Nagano, Katsumasa Goto, Kunihiro Sakuma, Hisashi Naito, Toshitada Yoshioka and Scott K. Powers, "Changes in PKB/Akt and calcineurin signaling during recovery in atrophied soleus muscle induced by unloading," American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, vol. 288, no. 5, pp. R1273-R1278, 2005   DOI
26 Sasai, N., Agata, N., Inoue-Miyazu, M., Kawakami, K., Kobayashi, K., Sokabe, M., and Hayakawa, K., "Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes," Muscle & nerve, vol. 41, no. 1, pp. 100-106, 2010.   DOI
27 OUWENS, D. M., WITHERS, D. J., ALESSI, D. R., and SHEPHERD, P. R., "Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation," Biochemical Journal, vol. 344, no. 2, pp. 427-431,1999.   DOI
28 Manning, Brendan D., and Lewis C. Cantley., "AKT/PKB signaling: navigating downstream," Cell, vol. 129, no. 7, pp. 1261-1274, 2007.   DOI
29 Robey, R. Brooks, and Nissim Hay. "Is Akt the "Warburg kinase"?-Akt-energy metabolism interactions and oncogenesis," Seminars in cancer biology, vol. 19, no. 1, pp. 25-31, 2009.   DOI
30 Shiojima, Ichiro, and Kenneth Walsh, "Role of Akt signaling in vascular homeostasis and angiogenesis," Circulation research, vol. 90, no. 12, pp. 1243-1250, 2002.   DOI
31 Uemura, M., Maeshige, N., Koga, Y., Ishikawa-Aoyama, M., Miyoshi, M., Sugimoto, M., and Usami, M, "Monophasic pulsed $200-{\mu}A$ current promotes galvanotaxis with polarization of actin filament and integrin ${\alpha}2{\beta}1$ in human dermal fibroblasts," Eplasty, vol. 16, 2016.
32 Baar, Keith, and Karyn Esser, "Phosphorylation of p70S6kcorrelates with increased skeletal muscle mass following resistance exercise," American Journal of Physiology-Cell Physiology, vol. 276, no. 1, pp. C120-C127, 1999.   DOI
33 Sue C. Bodine, Trevor N. Stitt, Michael Gonzalez, William O. Kline, Gretchen L. Stover, Roy Bauerlein, Elizabeth Zlotchenko, Angus Scrimgeour, John C. Lawrence, David J. Glass and George D. Yancopoulos, "Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo," Nature cell biology, vol. 3, no. 11, pp. 1014-1019, 2001.   DOI
34 Zanchi, Nelo Eidy, and Antonio Herbert Lancha, "Mechanical stimuli of skeletal muscle: implications on mTOR/p70s6k and protein synthesis," European journal of applied physiology, vol. 102, no. 3, pp. 253-263, 2008.   DOI
35 Rossen, J., "Introduction to Microcurrent and Guide to Its Greatest Effectiveness," Pengrove, Carliff, 1989.
36 Fukushima, K., Senda, N., Iuri, H., Tamai, Y., and Mauakami, "Studies of galvanotaxis of leukocytes," Medical Journal of Osaka University, vol. 4. no. 2-3, pp. 195-208, 1953.