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http://dx.doi.org/10.9718/JBER.2017.38.2.62

Pulsatility Estimation of a Pulsatile Decellularizing Device for the Fabrication of Organ Scaffold  

Kim, Dong Sun (Department of Mechanical and Biomedical Engineering, Kangwon National University)
Yang, Se-Ran (Department of Thoracic and Cardiovascular Surgery, Kangwon National University)
Park, Sung Min (Department of Thoracic and Cardiovascular Surgery, Kangwon National University)
Choi, Seong Wook (Department of Mechanical and Biomedical Engineering, Kangwon National University)
Publication Information
Journal of Biomedical Engineering Research / v.38, no.2, 2017 , pp. 62-73 More about this Journal
Abstract
To identify a solution for the restricted availability of healthy lungs and the high risk of immune rejections following organ transplantation, tissue engineering techniques for culturing lungs have been studied by many research groups. The most promising method for culturing lungs is the utilization of a bio-scaffold that was prepared using harvested organs from human donors or other animals by removing their original cells. In this study, a pulsatile perfusion pump was used to alleviate the cell removal effect with the high fluid-dynamic power of the perfusion stream during the decellularization process, while other conventional studies focused on chemical methods to identify efficient detergents. The purpose of this study was to analyze the developed device by using energy equivalent pressure (EEP), which is an indicator of pulsatility, to understand the characteristics of pulsatile energy transmitted according to the load size by using the artificial model and compare it with the measured EEP. The pulsatility of the device can be estimated with the concept of fluid-dynamic energy during a particular constant time period or fluid-dynamic power represented as EEP and EEP increment. Because the measured EEP of perfusion flow during decellularization can be changed by the amount of fluid leakage and the degree of clogging in the capillary vessels, EEP should be measured to determine whether the decellularization is progressing without problems. The decrement of EEP caused by the high perfusion resistance was observed from some experimental results that were obtained with artificial models. EEP can be used to monitor the decellularization process after analyzing the varying EEP according to the amount of load. It was confirmed that the EEP was maintained at a high level in the experiment using the harvested lungs from 12-13-week-old rats. In addition, it was confirmed that the cell removal time was faster than when continuous perfusion was performed. In this study, pulsatile power delivered to the lungs was measured to monitor the process of cell removal, and it serve as the evidence for efficient decellularization.
Keywords
Pulsatility; Decellularization; Scaffold; Energy Equivalent Pressure (EEP); Lung;
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