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http://dx.doi.org/10.9718/JBER.2017.38.1.1

Micro-threads of Cross-linked Hyaluronic Acid Hydrogel using a Microfluidic Chip  

Lee, Yun-Kyung (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University)
Lee, Kwang-Ho (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University)
Publication Information
Journal of Biomedical Engineering Research / v.38, no.1, 2017 , pp. 1-8 More about this Journal
Abstract
The successful synthesis of hyaluronic acid micro-threads is very promising approach for the broad application in tissue engineering such as dermal fillers. Because hyaluronic acid has the excellent biocompatibility and ability to maintain the moisture of up to several hundred times its own weight. In order to generate the hyaluronic acid micro-threads in microfluidic system, we employed two-phase flow microfluidic chip to make a rapid synthesis of the hyaluronic acid hydrogel. Hyaluronic acid was mixed with 0.02N NaOH solution and 1, 4-Butanediol diglycidyl ether (BDDE) solution and then injected into core channel. The ethanol was used for the 3-dimensional micro-thread formation in sheath channel. We manipulated the diameter of HA micro-threads using controlling of flow rates in microfluidic chip, and showed the feasibility of immobilization in HA micro-threads with florescent substances. Also, the generated HA micro-threads were evaluated and showed the suitable properties with tensile strength, bending property, and swelling profiles for dermal fillers. As a result, we suggested an innovative method for microfluidic chip-based HA micro-threads which could safely be applied as dermal filler in tissue engineering.
Keywords
hyaluronic acid; 1, 4-butanediol diglycidyl ether; dermal filler; immobilization; swelling; microfluidic chip;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 J.-Y. Kwon and S.-I. Cheong, "Characterization of hyaluronic acid membrane cross-linked with lactide," Polymer Korea, vol. 29, pp. 599-604, 2005.
2 J.T. Kim, D.Y. Lee, and J.H. Choi, "Preparation and characterization of hyaluronic acid microbeads," Biomater. Res, vol. 14, pp. 157-160, 2010.
3 W. Jang, S. Kim, I. Lee, M. Kim, J. Rhee, G. Khang, et al., "Neurogenesis of bone marrow stromal cell using controlled release of butylated hydroxyanisole from PLGA films," Tissue Eng Regen Med, vol. 2, pp. 100, 2005.
4 C.-Y. Choi, J.-K. Park, W.-S. Kim, M.-K. Jang, and J.-W. Nah, "Preparation and Characterization of Deoxycholic Acid-Grafted Hyaluronic Acid as a Durg Carrier," Polymer Korea, vol. 35, pp. 119-123, 2011.   DOI
5 J. Kim, J. Choi, and D. Lee, "Pyrogenicity of hyaluronic acid hydrogel crosslinked by divinyl sulfone for soft tissue augmentation," Natural Science, vol. 2, pp. 764, 2010.   DOI
6 J.T. Kim and J.H. Choi, "Production and evaluation of hyaluronic acid gel for soft tissue augmentation," Biomaterials Research, vol. 13, pp. 105-108, 2009.
7 J.-T. Kim, D.-Y. Lee, Y.-H. Kim, I.-K. Lee, and Y.-S. Song, "Effect of pH on Swelling Property of Hyaluronic Acid Hydrogels for Smart Drug Delivery Systems," Journal of Sensor Science and Technology, vol. 21, pp. 256-262, 2012.   DOI
8 I.-Y. Kim, S.-J. Seo, H.-S. Moon, M.-K. Yoo, I.-Y. Park, B.-C. Kim, et al., "Chitosan and its derivatives for tissue engineering applications," Biotechnology advances, vol. 26, pp. 1-21, 2008.   DOI
9 E. Song, S.Y. Kim, T. Chun, H.-J. Byun, and Y.M. Lee, "Collagen scaffolds derived from a marine source and their biocompatibility," Biomaterials, vol. 27, pp. 2951-2961, 2006.   DOI
10 O. Jeon, S.J. Song, K.-J. Lee, M.H. Park, S.-H. Lee, S.K. Hahn, et al., "Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densities," Carbohydrate polymers, vol. 70, pp. 251-257, 2007.   DOI
11 W.J. Jeong, J.Y. Kim, J. Choo, E.K. Lee, C.S. Han, D.J. Beebe, et al., "Continuous fabrication of biocatalyst immobilized microparticles using photopolymerization and immiscible liquids in microfluidic systems," Langmuir, vol. 21, pp. 3738-3741, 2005.   DOI
12 S. Zhang, M.A. Greenfield, A. Mata, L.C. Palmer, R. Bitton, J.R. Mantei, et al., "A self-assembly pathway to aligned monodomain gels," Nature materials, vol. 9, pp. 594-601, 2010.   DOI
13 S. Sugiura, T. Oda, Y. Aoyagi, M. Satake, N. Ohkohchi, and M. Nakajima, "Tubular gel fabrication and cell encapsulation in laminar flow stream formed by microfabricated nozzle array," Lab on a Chip, vol. 8, pp. 1255-1257, 2008.   DOI
14 S.A. Zawko, S. Suri, Q. Truong, and C.E. Schmidt, "Photopatterned anisotropic swelling of dual-crosslinked hyaluronic acid hydrogels," Acta Biomaterialia, vol. 5, pp. 14-22, 2009.   DOI
15 Y. Jun, M.J. Kim, Y.H. Hwang, E.A. Jeon, A.R. Kang, S.-H. Lee, et al., "Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection," Biomaterials, vol. 34, pp. 8122-8130, 2013.   DOI
16 I.-R. Hong and Y.-J. Kim, "Self-Aggregated Nanoparticles of Lipoic Acid Conjugated Hyaluronic Acid," Polymer Korea, vol. 32, pp. 561-565, 2008.
17 Y. Xia and G.M. Whitesides, "Soft lithography," Annual review of materials science, vol. 28, pp. 153-184, 1998.   DOI
18 B. Hafner, "Scanning electron microscopy primer," Characterization Facility, University of Minnesota-Twin Cities, pp. 1-29, 2007.
19 H.-Y. Lee, S.-H. Jeong, J.-U. Baek, J.-H. Song, and H.-E. Kim, "Mechanical Improvement of Hyaluronic Acid (HA) Hydrogels and Incorporation of Polyethylene Glycol (PEG)." Biometaerials, 2015.
20 박병규, "조직 공학용 생분해성 고분자 : 총설," Journal of Biomedical Engineering Research, vol. 36, pp. 251-263, 2015.   DOI
21 M. Al-Sibani, A. Al-Harrasi, and R.H. Neubert, "Evaluation of in-vitro degradation rate of hyaluronic acid-based hydrogel cross-linked with 1, 4-butanediol diglycidyl ether (BDDE) using RP-HPLC and UV-Vis spectroscopy," Journal of Drug Delivery Science and Technology, vol. 29, pp. 24-30, 2015.   DOI
22 A.P. Sclafani and T. Romo III, "Collagen, human collagen, and fat: the search for a three-dimensional soft tissue filler," Facial plastic surgery, vol. 17, pp. 079-085, 2001.   DOI
23 K. Kofuji, T. Ito, Y. Murata, and S. KAWASHIMA, "The controlled release of a drug from biodegradable chitosan gel beads," Chemical and Pharmaceutical Bulletin, vol. 48, pp. 579-581, 2000.   DOI
24 Y.-H. Kim, I.-K. Lee, J.-T. Kim, J.-H. Park, and D.Y. Lee, "Dependence of Crosslinking Temperature on Swelling Behavior of Hyaluronic Acid Porous Microbeads Synthesized by a Modified Spray Method," Journal of the Korean Ceramic Society, vol. 49, pp. 518-522, 2012.   DOI
25 F. Roig-Roig, C. Solans, J. Esquena, and M.J. Garcia-Celma, "Preparation, characterization, and release properties of hydrogels based on hyaluronan for pharmaceutical and biomedical use," Journal of Applied Polymer Science, vol. 130, pp. 1377-1382, 2013.   DOI
26 J. Yeom, S.H. Bhang, B.-S. Kim, M.S. Seo, E.J. Hwang, I.H. Cho, et al., "Effect of cross-linking reagents for hyaluronic acid hydrogel dermal fillers on tissue augmentation and regeneration," Bioconjugate chemistry, vol. 21, pp. 240-247, 2010.   DOI
27 G.D. Prestwich, D.M. Marecak, J.F. Marecek, K.P. Vercruysse, and M.R. Ziebell, "Controlled chemical modification of hyaluronic acid: synthesis, applications, and biodegradation of hydrazide derivatives," Journal of Controlled Release, vol. 53, pp. 93-103, 1998.   DOI
28 S.-N. Park, H.J. Lee, K.H. Lee, and H. Suh, "Biological characterization of EDC-crosslinked collagen-hyaluronic acid matrix in dermal tissue restoration," Biomaterials, vol. 24, pp. 1631-1641, 2003.   DOI
29 C. Mao and W.S. Kisaalita, "Characterization of 3-D collagen hydrogels for functional cell-based biosensing," Biosensors and Bioelectronics, vol. 19, pp. 1075-1088, 2004.   DOI
30 M. Lerner, "Hyaluronic acid makers enjoy growth from old and new uses," Chemical market reporter, vol. 252, pp. 12, 1997.
31 J. Park, "Biomaterials: Science and Engineering Plenum Press," New York, 1984.
32 K.H. Lee, S.J. Shin, C.B. Kim, J.K. Kim, Y.W. Cho, B.G. Chung, et al., "Microfluidic synthesis of pure chitosan microfibers for bio-artificial liver chip," Lab on a Chip, vol. 10, pp. 1328-1334, 2010.   DOI
33 R. Langer and D.A. Tirrell, "Designing materials for biology and medicine," Nature, vol. 428, pp. 487-92, Apr 1 2004.   DOI
34 B.G. Chung, K.H. Lee, A. Khademhosseini, and S.H. Lee, "Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering," Lab on a Chip, vol. 12, pp. 45-59, 2012.   DOI
35 I. Park, S.H. Oh, and J.H. Lee, "Fabrication and characterization of pore size gradient alginate scaffold by a centrifugation method," Tissue Eng Regen Med, vol. 1, pp. 164, 2004.