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http://dx.doi.org/10.9718/JBER.2015.36.6.296

Effect of Cytosine Arabinoside Pre-treatment in Dorsal Root Ganglion Explant Culture  

Jung, H.Y. (Department of Biomedical Engineering, Korea Institute of Science and Technology)
Han, S. (Department of Biomedical Engineering, Korea Institute of Science and Technology)
Park, J.W. (Department of Biomedical Sciences, Korea University College of Medicine)
Youn, I. (Department of Biomedical Engineering, Korea Institute of Science and Technology)
Publication Information
Journal of Biomedical Engineering Research / v.36, no.6, 2015 , pp. 296-301 More about this Journal
Abstract
Explant culture condition of dorsal root ganglion have been used to investigate the pathophysiology of peripheral nerve injury, while applying for the various clinical symptom such as trauma, pressure, and stretch. However, explant culture is usually contaminated by mitotic cells, which may observed as a newly divided cells including fibroblast or glia. The mitotic cells could be able to interrupt and change the cell signaling that make it difficult to avoid detrimental effects during the experiments. To eliminate mitotic cells, anti-mitotic reagents like mixture of uridine and 5-fluorodeoxyuridine or cytosine arabinoside were added to the cultures on the following day, but there is no research that investigate viability of anti-mitotic reagent in dorsal root ganglion explant culture. In this study, we investigate inhibition effect of cytosine arabinoside to mitotic cells in dorsal root ganglion explant culture. Also we visualized and analyzed anti-mitotic effect and toxicity of cytosine arabinoside in various concentration condition. This dorsal root ganglion explant culture condition can be applied to research that effect and mechanism of various stimulation and chemical application which affect peripheral nerve regeneration.
Keywords
Dorsal root ganglion explant culture; Cytosine arabinoside; Antimitotic effect; Neurite regeneration;
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