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http://dx.doi.org/10.9718/JBER.2013.34.3.117

Characterization of Cross Linked Hyaluronic Acid Microbeads by Divinyl Sulfone  

Kim, Jin-Tae (Department of Advanced Material Engineering, Chungbuk National University)
Lee, Deuk Yong (Department of Material Engineering, Daelim University)
Jang, Ju-Woong (Cellumed Co., Ltd.)
Kim, Tae-Hyung (Department of Radiological Science, Kangwon National University)
Jang, Yong-Wun (Department of Material Engineering, Daelim University)
Publication Information
Journal of Biomedical Engineering Research / v.34, no.3, 2013 , pp. 117-122 More about this Journal
Abstract
Hyaluronic acid(HA) microbeads were synthesized by dropping the sodium hyaluronate(Streptococcus) solutions in NaOH into a solution mixture of divinyl sulfone(DVS) in 2-methyl-1-propanol, followed by stirring, cleaning and drying process at room temperature. The initial experimental conditions are crosslinking time(CLTi) of 5 h, crosslinking temperature(CLTe) of room temperature, injection air pressure(IAPr) of 5 psi, and DVS concentration( DVSc) of 0.2 vol%, respectively. Then, parametric studies were performed by varying the parameters to investigate the morphology, the porosity, the swelling ratio and the size of the beads. The microbead size pattern was not regular to function of the degree of crosslink. It was observed that the swelling ratio, the degree of crosslink, and the pore size can be controlled by adjusting the CLTi, CLTe and DVSc. Among the parameters investigated, the smallest bead size can be achieved by varying the CLTi parameter. The lowest swelling ratio, as an indication of the highest degree of crosslink, can be obtained by varying CLTe.
Keywords
Hyaluronic acid microbead; Tissue regeneration; Hydrogel;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Ji Young Kwon, Seong Ihl Cheong, "Synthesis and Characteristics of Hyaluronic Acid Bead Crosslinked by 1,3-Butadiene diepoxide", Polymer(Korea), vol. 29, no. 5, pp. 445- 450, 2005.   과학기술학회마을
2 Chirag B. Shah, Stanley M. Barnett, "Swelling Behavior of Hyaluronic Acid Gels", J. of Applied Polymer Science, vol. 45, pp. 293-298, 1992.   DOI
3 F.A. Korkoosh, J. Brussee, J.C. Verhoef, F. Borchard, M. Rafiee-Tehrani, H.E. Junginger, "Preparation and NMR characterization of superporous hydrogels(SPH) and SPH composites", Polymer, vol. 41, pp. 8213-8220, 2000.   DOI   ScienceOn
4 Jun Chen, Haesun Park, Kinam Park, "Synthesis of superporous hydrogels: Hydrogels with fast swelling and superabsorbent properties", J. of Biomed. Mater. Res., vol. 44, pp. 53-62, 1999.   DOI   ScienceOn
5 Richard A. Gemeinhart, Haesun Park and Kinam Park, "Pore Structure of Superporous Hydrogels", Polym. Adv. Technol., vol. 11, pp. 617-625, 2000.   DOI   ScienceOn
6 Hossein Omidian, Jose G. Rocca, Kinam Park, "Advances in superporous hydrogels", J. of Controlled Release, vol. 102, pp. 3-12, 2005.   DOI   ScienceOn
7 Hetal Patel, Minal Bonde, Ganga Srinivasan, "Biodegradable Polymer Scaffold for Tissue Engineering", Trends Biomater. Artif. Organs, vol. 25, no. 1, pp. 20-29, 2011.
8 Jin Tae Kim, Deuk Yong Lee, Jae Ha Choi, "Short Term Toxicity test of Tissue Augmentation Materials by Hyaluronic Acid Micro Bead", Biomaterials Research, vol. 14, no. 1, pp. 25-29, 2010.
9 Jin Tae Kim, Deuk Yong Lee, Jae Ha Choi, "Implanting Test of Re-hydrated Hydrogels by Hyaluronic acid Microbeads", Biomaterials Research, vol. 15, no. 3, pp. 125-128, 2011.
10 Jin Tae Kim, Jae Ha Choi, "Production and Evaluation of Hyaluronic Acid Gel for Soft Tissue Augmentation", Biomaterials Research, vol. 13, no. 3, pp. 105-108, 2009.
11 J.T. Kim, C.H. Kook, J.H. Choi, "Production Equipment and Method of Polymer Gel for Bio-Implanting", J. of Korean Soc. of Mechanical Technology, vol. 11, no. 3, pp. 89-94, 2009.   DOI
12 "Manufacturing process of cationic polymer/hyaluronic acid microbead and cationic polymer/hyaluronic acid microbead chelated with metal ion highly containing hyaluronic acid in the inside of microbead, and cationic polymer/hyaluronic acid microbead chelated with metal ion manufactured by the same" 1020110135690A, patent of Korea.
13 "Microbeads of natural polysaccharide and hyaluronic acid and processes for preparing the same", PCT/KR2003/001716.
14 "Microbead of hyaluronic acid derivatives for medical purpose and process for preparing the same", 1020070012002, patent of Korea.
15 J.T. Kim, D.Y. Lee, Y.S. Oh, J.W. Bang, C.Y. Hyun, T.H. Kim, "Swelling Properties for Cross-Linking Degree of Hyaluronic Acid Hydrogels", Biomaterials Research, vol. 17, no. 1, pp. 37-40, 2013.
16 Chiara Schiraldi, Annalisa La Gatta and Mario De Rosa, "Biotechnological Production and Application of Hyaluronan", In Biopolymers; Elnashar, M., Ed.; Rijeka, Croatia, 387-412, 2010.
17 S.P. Zhong, D. Campoccia, P.J. Doherty, R.L. Williams, L. Benedetti, D.F. Williams, "Biodegradation of hyaluronic acid derivative by hyaluronidase", Biomaterials. vol. 15, no. 5, pp. 359-365, 1994.   DOI   ScienceOn
18 Y. Tokita, A. Okamoto, "Hydrolytic degradation of hyaluronic acid", Polymer Degradation and Stalility. vol. 48, no. 2, pp. 269-273, 1995.   DOI   ScienceOn
19 A.M. Afify, M. Stern, M. Guntenhoner, R. Stern, "Purification and characterization of human serum hyaluronidase", Biochem Biophys. vol. 305, no. 2, pp. 434-441, 1993.   DOI   ScienceOn
20 Oju Jeon, Su Jin Song, Kee-Jung Lee, Moon Hyang Park, Soo-Hong Lee, Sei Kwang Hahn, Sungjee Kim, Byung-soo Kim, "Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densites", Carbohydrate Polymers, vol. 70, pp. 251-257, 2007.   DOI   ScienceOn
21 Si-Nae Park, Jong-Chul Park, Hea Ok Kim, Min Jung Song, Hwal Suh, "Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide cross-linking", Biomaterials, vol. 23, pp. 1205-1212, 2002.   DOI   ScienceOn
22 Yi Luo, Kelly R. Kirker, Gelnn D. Prestwich, "Cross-linked hyaluronic acid hydrogel films: new biomateirals for drug delivery", J. of Controlled Release, vol. 69, pp. 169-184, 2000.   DOI   ScienceOn
23 Xiao Zheng Shu, Yanchun Liu, Yi Luo, Meredith C. Roberts, Glenn D. Prestwich, "Disulfied Cross-Linked Hyaluronan Hydrogels", Biomacromolecules, vol. 3, pp. 1304-1311, 2002.   DOI   ScienceOn
24 Jin-Tae Kim, Jae-Ha Choi, Deuk-Yong Lee, "Pyrogenicity of hyaluronic acid hydrogel cross-linked by divinyl sulfone for soft tissue augmentation", Natural Science, vol. 2, no. 7, pp. 764-768, 2010.   DOI
25 Sei Kwang Hahn, Jung Kyu Park, Takashi Tomimatsu, Tsuyoshi Shimoboji, "Synthesis and degradation test of hyaluronic acid hydrogels", Inter. J. of Biological Macromolecules, vol. 40, pp. 374-380, 2007.   DOI   ScienceOn
26 Kenji Tomihata, Yoshito Ikada, "Preparation of cross-linked hyaluronic acid films of low water content", Biomaterials, vol. 18, pp. 189-195, 1997.   DOI   ScienceOn
27 Jin Tae Kim, Deuk Yong Lee, Jae Ha Choi, "Preparation and Characterization of Hyaluronic Acid Microbeads", Biomaterials Research, vol. 14, no. 4, pp. 157-160, 2010.
28 A. Pourjavadi, M. Ayyari, M.S. Amini-Fazl, "Taguchi optimized synthesis of collagen-g-poly(acrylic acid)/kaolin composite superabsorbent hydrogel", European Polymer Journal, vol. 44, pp. 1209-1216, 2008.   DOI   ScienceOn