Browse > Article
http://dx.doi.org/10.9718/JBER.2010.31.3.234

Development of Diagnosis Protocol for Micro-spike Biopsy Using Paraffin-based Tissue Collecting tool  

Jeong, Hyo-Young (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Koo, Kyo-In (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Lee, Sang-Min (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Park, Ho-Soo (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Hong, Suk-Jun (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Bang, Seoung-Min (Department of Internal Medicine, College of Medicine, Yonsei University)
Song, Si-Young (Department of Internal Medicine, College of Medicine, Yonsei University)
Cho, Dong-Il (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
Publication Information
Journal of Biomedical Engineering Research / v.31, no.3, 2010 , pp. 234-239 More about this Journal
Abstract
We have developed and reported several micro-spikes for minimally invasive biopsy. This paper presents a diagnosis protocol for micro-spike biopsy using paraffin-based tissue collecting tool. Using the proposed tissue collecting tool, which has a negative micro-spike structure in a porous chamber, the biopsied tissue in a micro-spike is effectively detached. The proposed diagnosis protocol prevents the loss of tissues in a paraffin embedding and sectioning process. Hence, it is compatible with conventional histopathology without additional reagents and processes. The gastro-intestinal tissue of a pig is biopsied in an in vivo environment, and then it is detached from a micro-spike using the paraffin-based tissue collecting tool. A histopathological photomicrograph of the detached tissue is acquired with the proposed diagnosis protocol. The acquired image offers clinical quality. This result shows that the paraffin-based tissue collecting tool is applicable to the medical practice.
Keywords
micro-spike; paraffin-based tissue collecting tool; detaching tool; biopsy; diagnosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 S. Byun, J. M. Lim, S. J. Palk, A. Lee, K. Koo, S. Park, J. Park, B. D. Choi, J. M. Seo, K. Kim, H. Chung, S. Y. Song, D. Jeon, and D. "Dan" Cho, "Barbed micro-spikes for micro-scale biopsy," J. Micromech. Microeng., vol. 15, no. 6, pp. 1279-1284, 2005.   DOI   ScienceOn
2 G. S. Kim, S. Park, K. Koo, M. Jeong, H. Choi, S. Paik, S. M. Bang, S. Y. Song, and D. "Dan" Cho, "Micro spike for endoscopic gastro biopsy," Asia-Pacific Conference of Transducers and Micro-Nono Technology, Singapore, Singapore, Jun. 2006.
3 H.Y. Jeong, K. I. Kyo, S. M. Lee, J. W.Ban, H. S. Park, S. M. Bang, S.Y. Song, and D. I. Cho, " Micro-tissue Collecting Tool for Diagnosis of Micro-spike Biopsy," Journal of the Korean Sensors Society, vol. 18, no. 2, pp. 122-127, 2009.   과학기술학회마을
4 S.I. Kim, Y.C. Shin, Y. S. Yang, G. Y Oh, Histopathology, Seoul, Daehakseorim, Press, 1995, pp. 41-48, pp. 155-164.
5 Becker E. W., Ehrfeld W., Hagmann P., Maner A, and Munchmeyer D., "Fabrication of microstructures with high aspect ratios and great structural heights by synchrotron radiation lithography, galvanoforming, and plastic moulding (LIGA process)", Microelectronic Eng., vol.4, no. 1, pp. 35-56, 1986.   DOI   ScienceOn
6 K. L. Woods, B. S. Anand, R. A. Cole, M. S. Osato, Robert, M. Genta, H. Malaty, I. E. Gurer, and D. De Rossi, "Influence of endoscopic biopsy forceps characteristics on tissue specimens : results of a prospective randomized study," Gastrointestinal endoscopy, vol. 49, no. 2, pp. 177-183, 1999.   DOI   ScienceOn
7 DD. Wadas, and R.A. Sanowski, "Complications of the hot biopsy forceps technique," Gastrointestinal endoscopy, vol. 34, no. 1, pp. 32-39, 1988.   DOI   ScienceOn
8 S. K. Park, A. R, Lee, M. J. Jeong, H. M. Choi, S. Y. Song, S. M. Bang, S. J. Paik, J. M. Lim, D. Y. Jean, S. K. Lee, C N. Chu, and D. I. Cho, "A Disposable MEMS-Based Micro-Biopsy Catheter for the Minimally Invasive Tissue Sampling," IEEE/RSJ international Conference on Intelligent Robots and Systems, Alberta, Canada, Aug. 2005.
9 A. R. Lee, S. J. Paik, K. I. Koo, S. G. Park, B. D. Choi, M. J. Jeong, H. M. Choi, J. M. Lim, J. M. Seo, K. A Kim, H. Chung, D. Y. Jeon, S. S. Lee, and D. I. Cho, "A Novel Microbiopsy Tool for Microsurgery," in Proc. of the Korean Sensors Society Conference, Cheju, Korea, Nov. 12-13, 2004, pp. 154-155.
10 A. R. Lee, S. J. Paik, S. G. Park, M. J. Jeong, K. I. Koo, H. M. Choi, J. M. Seo, K. A. Kim, H., Chung, S. Y. Song, S. M. Bang, D. Y. Jeon, J. M. Lim, and D. L Cho, "3D Silicon-micromachined Microbiopsy Tool and In-vivo Experiment," The 13th international Conference on Solid-State Sensors, Actuators and Microsystems, Seoul, Korea, Jun. 2005. pp. 1199-1202.
11 G. S. Kim, S. K. Park, K l., Koo, H. M. Chai, M. J. Jung, S. Y. Song, S. M. Bang, and D. I. Cho, "Nickel Micro-spike for Micro-scale Biopsy Using LiGA Process," The 9th International Conference on Synchrotron Radiation Instrumentation, Daegu, Korea, May. 2006, pp. 1449-1502.
12 Donald C. Zavola, "Diagnostic fiberoptic bronchoscopy: Techniques and results of biopsy in 600 patients," Chest, vol. 68, no. 1, pp. 12-19, 1975.   DOI   ScienceOn