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http://dx.doi.org/10.9718/JBER.2010.31.1.081

Effect of Low-Energy Laser Irradiation on the Proliferation and Gene Expression of Myoblast Cells  

Kwag, J.H. (Department of Biomedical Engineering, Yonsei University)
Jeon, O.H. (Department of Biomedical Engineering, Yonsei University)
Kang, D.Y. (Department of Biomedical Engineering, Yonsei University)
Ryu, H.H. (Constitutional Biology and Medical Engineering Research Center, Korea Institute of Oriental Medicine)
Kim, K.H. (Department of Biomedical Engineering, Yonsei University)
Jung, B.J. (Department of Biomedical Engineering, Yonsei University)
Kim, C.H. (Department of Biomedical Engineering, Yonsei University)
Publication Information
Journal of Biomedical Engineering Research / v.31, no.1, 2010 , pp. 81-86 More about this Journal
Abstract
Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. $C_2C_{12}$ myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.
Keywords
Low-energy laser irradiation; myoblast; cell proliferation; MyoD; Myf5; VEGF;
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