Browse > Article
http://dx.doi.org/10.9718/JBER.2007.28.4.512

Investigation of Nanofiber and Thermosensitive Scaffold for Intervertebral Disc through Organ Culture  

Lee, Yong-Jae (Department of Biomedical Engineering, Inje University)
Shin, Ji-Won (Department of Biomedical Engineering, Inje University)
Shin, Ho-Jun (School of Material Science, Japan Advanced Institute of Science and Technology)
Kim, Chan-Hwan (Department of Pathology, Inje University)
Park, Ki-Dong (Department of Molecular Science and Technology, Ajou University)
Bae, Jin-Woo (Department of Molecular Science and Technology, Ajou University)
Seo, Hyoung-Yeon (Department of Orthopaedic Surgery, College of Medicine, Chunnam National University)
Kim, Young-Jick (Department of Dental Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Shin, Jung-Woog (Department of Biomedical Engineering, Inje University)
Publication Information
Journal of Biomedical Engineering Research / v.28, no.4, 2007 , pp. 512-519 More about this Journal
Abstract
The purpose of this study is to investigate the potential of a novel tissue engineering approach to regenerate intervertebral disc. In this study, thermosensitive scaffold (chitosan-Pluronic hydrogel) and nanofiber were used to replace the nucleus pulposus (NP) and annulus fibrosus of a degenerated intervertebral disc, leading to an eventual regeneration of the disc using the minimally invasive surgical procedure and organ culture. In preliminary study, disc cells were seeded into the scaffolds and cellular responses were assessed by MTT assay and scanning electron microscopy (SEM). Based on these results, we could know that tissue engineered scaffolds might provide favorable environments for the regeneration of tissues. Organ culture was performed in fresh porcine spinal motion segments with endplates on both sides. These spinal motion segments were classified into three groups: control (Intact), injured NP (Defect), and inserting tissue engineered scaffolds (Insert). The specimens were cultivated for 7 days, subsequently structural stability, cell proliferation and morphological changes were evaluated by the relaxation time, quantity of DNA, GAG and histological examination. In these results, inserting group showed higher relaxation time, reduced decrement of DNA contents, and accumulated GAG amount. Consequently, the tissue engineered scaffolds used in this study seen to be a promising base scaffolds for regenerative intervertebral disc due to its capacity to absorb external dynamic loading and the possible ideal environment provided for disc cell growing.
Keywords
intervertebral disc; regeneration; chitosan-Pluronic hydrogel; nanofiber; organ culture;
Citations & Related Records
연도 인용수 순위
  • Reference
1 J. S. Ahn, J. K. Lee, T. S. Jeon, S. Y. Kwon and S. K. Kwak, 'Correlation among magnetic resonance images, electron microscopic finding, light microscopic findings and clinical symptom of the degeneration of lumbar intervertebral disc,' J. Korean Soc. Spine Surg., vol. 8, no. 2. pp. 121-129, 2001   DOI
2 B. Shen, J. Melrose, P. Ghosh and F. Tayler, 'Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in theree-dimensional agarose gel culture by interleukin-$1{\beta}$: a potential pathway o disc degeneration,' Eur. Spine J., vol. 12, pp. 66-75, 2003   PUBMED
3 J. A. Buckwalter, T. A. Einhorn and S. R. Simon, 'Orthopaedic basic sicence 2nd. American academy of orthopaedic surgeons,' Illinois, pp. 443, 2000
4 G. D. Palmer, Ph P. H. Chao, F. Raia, R. L. Mauck, W. B. Valhmu and C. T. Hung, 'Time-dependent aggrecan gene expression of articular chondrocytes in reponse to hyperosmotic loading,' Osteoarthritis cartilage, vol. 9, no. 8, pp. 761-770, 2001   DOI   ScienceOn
5 W. Johannessen, E. J. Vresilovic, A. C. Wright, D. M. Elliott, 'Intervertebral disc mechanics are restrored following cyclic loading and unloaded recover,' Annals of Biomedical Engineering, vol. 32, no. 1, pp. 70-76, 2004   DOI   ScienceOn
6 K. Takegami, H. S. An, F. Kumano, K. Chiba, E. J. Thonar, K. Singh and K. Masuda, 'Osteogenic protein-I is most effective in stimulating nucleus pu1posus and annulus fibrosus cells to repair their matrix after chondroitinase ABC-induced in vitro chemonucleolysis,' Spine J, vol. 5, no. 3, pp. 231-238, 2005   DOI   ScienceOn
7 T. Chujo, H. S. An, K. Akeda, K. Miyamoto, C. Muehleman, M. Attawia, G. Andersson and K. Masuda, 'Effects of growth differentiation of factor-5 on the intervertebral disc - In vitro bovine study and in vivo rabbit disc degeneration model study,' Spine, vol. 31, no. 25, pp. 2909-2917, 2006   DOI   ScienceOn
8 S. J. Chacko, J. Abbott S. and Holtzer, 'The loss of phenotypic traits by differentiated cells. VI. Behavior of the progeny of a single chondrocyte,' J. Exp. Med., vol. 130, pp. 417-442, 1969   DOI
9 J. W. Lee, M. C. Jung, H. D. Park, K. D. Park and G. H. Ryu, 'Synthesis and characterization of thermosensitive chitosan copolymer as a novel biomaterial,' J. Biomater Sci. Polym. Ed., vol. 15, no. 8, pp. 1065 - 1079, 2004   DOI
10 S. Stern, K. Lindenhayn, O. Schultz and C. Perka, 'Cultivation of porcine cells from the nucleus pulposus in a fibrin/hyaluronic acid matrix,' Acta Orthop Scand, vol. 71, no. 5, pp. 496-502, 2000   DOI
11 H. Ishihara, K. Warensjo, S. Roberts and J. P.Urban, 'Proteoglycan synthesis in the intervertebral disk nucleus: the role of extracellular osmolaity,' Am. J. Physiol., vol. 272, pp. 1499-1506, 1997   DOI
12 C. R. Lee, J. C. Latridis, L. Poveda and M. Alini, 'In vitro organ culture of the bovine intervertebral disc - Effects of vertebral endplate and potential for mechanobiology studies,' Spine, vol. 31,no. 5,pp. 515-522, 2006   DOI   ScienceOn
13 J. C. Gan, P. Ducheyne, E. J. Vresilovic, W. Swaim and I. M. Shapiro, 'Intervertebral disc tissue engineering I : Characterization of the nucleus pulposus,' Clinical Orthop Related Res, vol. 411, pp. 305-314, 2003   DOI   ScienceOn
14 H. E. Gruber, G. L. Hoeslscher, K. Leslie, J. A. Ingram and E. N. Hanley, 'Three-dimensional culture of human disc cells within agarose or a collagen sponge: assessment of proteoglycan production,' Biomaterials, vol. 27, pp. 371-376, 2006   DOI   ScienceOn
15 D. Haschtmann, J. V. Stoyanov, L. Ettinger, L. P. Nolte and S. J. Ferguson, 'Establishment of a novel intervertebral disc/endplate culture model: Analysis of an ex vivo in vitro whole-organ rabbit culture system,' Spine, vol. 31, no. 25, pp. 2918-2925, 2006   DOI   ScienceOn
16 M. Okuma, J. Mochida, K. Nishimura, K. Sakabe and K. Seiki, 'Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: an in vitro and in vivo experimental study,' J. Orthop. Res., vol. 18, pp. 988 - 997, 2000   DOI   ScienceOn
17 J. C. Lotz, 'Animal models of intervertebral disc degeneration: Lessons learned,' Spine, vol. 24, no. 23, pp. 2742-2750, 2004
18 M. V. Risbud, M. W. Izzo, C. S. Adams and et al. 'An organ culture system for the study of the nucleus pulposus: description of the system and evaluation of the cells,' Spine, vol. 28, no. 24, pp. 2652-2659, 2003   DOI   ScienceOn
19 Y. T. Kim, J. J. Kim, J. H. Hwang, M. J. Shin, E. S. Yu and Y. K. Shin, 'Annulus tears in the intervertebral disc degeneration-An experimental study using an animal model,' J. of Korean Spine Surg, vol. 1, pp. 259-272, 1994
20 R. Q. Brown, A. Mount and K. J. L. Burg, 'Evaluation of polymer scaffolds to be used in a composite injectable system for intervertebral disc tissue engineering,' J. Biomed. Mater. Res. A, vol. 74, no. 1, pp. 32-39, 2005   PUBMED
21 S. Sobajima, J. F. Kompel, J. S. Kim, C. J. Wallach, D. D. Robertson, M. T. Vogt. J. D. Kangand L. G. Gilbertson, 'A slowly progressive and reproducible animal model of intervertebral disc degeneration characterized by MRI, X-ray, and histology,' Spine, vol. 30, no. 1, pp. 15-24, 2005   DOI   PUBMED
22 H. S. An, E. J. Thonar and K. Masuda, 'Biological repair of intervertebral disc,' Spine, vol. 28, no. 15S, pp. S86-S92, 2003   DOI   ScienceOn
23 Y. S. Park, J. L. Cho and J. P. Kostuik, 'Experimental annulotomy-induced degeneration in rabbit intervertebral discs -Comparative study between incomplete and complete annulotomy,' J. of Korean Orthop Accoc, vol. 38, pp. 619-623, 2003   DOI
24 C. Park, Y. J. Kim, C. S. Lee, K. An, H. J. Shin, C. H. Lee, C. H. Kim and J. W. Shin, 'An in vitro animal study of the biomechanical responses of anulus fibrosus with aging,' Spine, vol. 30, no. 10, pp. E259-265, 2005   DOI   ScienceOn