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http://dx.doi.org/10.9718/JBER.2007.28.3.377

New Fluid Flow System for Simulation of Mechanical Loading to Bone Cells During Human Gait Cycle  

Ahn, Jae-Mok (Biomedical Engineering Lab. Department of Electronic Engineering, Hallym University)
Publication Information
Journal of Biomedical Engineering Research / v.28, no.3, 2007 , pp. 377-386 More about this Journal
Abstract
Mechanical loading to bone cells using simple sine wave or constant wave fluid flow has been widely used for in vitro experiments. Human gait is characterized by a complex loading to bones of lower extremities which results from a series of events consisting of heel strike, foot flat and push-off during the stance phase of the gait cycle. Telemetric force analyses have shown that human femora are subject to multiphasic loading. Therefore, it would be ideal if the physiologic loading conditions during human walking can be used for in vitro mechanotransduction studies. Here, for a mechanotransduction study, we develop it fluid flow system (FFS) in order to simulate human physiologic mechanicalloading on bone cells. The development methods of the FFS including the COR (Center for Orthopedic Research), monitor program are presented. The FFS could generate various multiphasic loading conditions of human gaits with output flow. Wall shear distribution was very uniform, with 81 % of the effective loading area of the culture on a glass slide. Our results demonstrated that the FFS, provide a new translational approach for unveiling molecular mechanotransduction pathways in bone cells.
Keywords
mechanotransduction; mechanical loading; wall shear; human gait; bone cells;
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