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http://dx.doi.org/10.9718/JBER.2007.28.2.244

Phase-Separation Properties of Poly(Ethylene Glycol) had Dextran Solutions In Microfluidic Device  

Choi, Joo-Hyung (Department of Biological Engineering, Inha University)
Chang, Woo-Jin (ERC for Advanced Bioseparation Technology, Inha University)
Lee, Sang-Woo (Department of Biomedical Engineering)
Publication Information
Journal of Biomedical Engineering Research / v.28, no.2, 2007 , pp. 244-249 More about this Journal
Abstract
Fluidic conditions for the separation of phases were surveyed in a microfluidic aqueous two-phase extraction system. The infusion ratio between polyethylene glycol (PEG) and dextran solution defines the concentrations of each polymer in micro-channel, which determine the phase-separation. The appropriate ratio between PEG (M.W. 8000, 10%, w/v) and dextran T500 (M.W. 500000, 5%, w/v) in order to perform the separation of phases of both polymers was observed as changing the mixed ratio of both polymers. Based on the fluidic conditions, stable two-phase solutions were obtained within 4% to 8% and 3% to 1% of PEG and dextran, respectively. In addition, the characteristics of the two-phase were discussed. The separation technique studied in the paper can be applied for the implementation of a lab-on-a chip which can detect various biological entities such cells, bacterium, and virus in an integrated manner using built in a biosensor inside the chip.
Keywords
microfluidic device; phase-separation; aqueous two-phase; extraction;
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