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http://dx.doi.org/10.9718/JBER.2006.27.6.409

Total Activity Estimation of Hippocampal Slice Using Multi-Electrode Array  

Lee, Jeong-Chan (Department of Biomedical Engineering, Kyung Hee University)
Kim, Ji-Eun (Department of Biomedical Engineering, Kyung Hee University)
Cho, Chung-Yearn (Department of Medical Science, Graduate School of East-West Medical Science)
Son, Min-Sook (Department of Medical Science, Graduate School of East-West Medical Science)
Park, Kyung-Mo (Department of Biomedical Engineering, Kyung Hee University)
Park, Ji-Ho (Department of Medical Science, Graduate School of East-West Medical Science)
Publication Information
Journal of Biomedical Engineering Research / v.27, no.6, 2006 , pp. 409-417 More about this Journal
Abstract
Research on neural circuit is a difficult area due to complexity and inaccessibility. Due to recent developments, the research using multi-electrode array of cells or tissues has become an important research area. However, there are some difficulties to decode the submerged meaning from huge and complex neural data. Moreover, it needs a harmonic collaboration between informatics and bioscience. In this paper, we have developed a custom-designed signal processing technique for multi-electrode array measured neural responses induced by electrical stimuli to the hippocampal tissue slices of the rat brain. The raw data from hippocampal slice using the multi-electrode array system were saved in a computer. Then we estimated characteristic points in each channel and calculated the total activity. To estimate the points, we used the Polynomial Fitting Approximation Method. Using the calculated total activity, we could provide the histogram or pseudo-image matrix to help interpretation of results.
Keywords
multi-electrode array; brain slice; hippocampus; total activity; polynomial fitting;
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1 Ray AM, Owen DE, Evans ML, Davis JB, Benham CD, 'Caspase inhibitors are functionally neuroprotective against oxygen glucosedeprivation induced CA1 death in rat organotypic hippocampus slice,' Brain Res., vol. 867, pp.62-69, 2000   DOI   ScienceOn
2 Martin P, Berthoz A, 'Development of spatial firing in the hippocampus of young rats,' Hippocampus, vol. 12, pp.465-480, 2002   DOI   ScienceOn
3 Shimono, K, Brucher F, Granger R, Lynch G, Taketani M, 'Origins and distribution of cholinergically induced $\beta$rhythms in hippocampal slices,' J. Neurosci., vol. 20, pp.8462-8473, 2000   DOI
4 Stett A, Egert U, Guenther E, Hofmann F, Meyer T, Nisch W, Haemmerle H, 'Biological application of microelectrode arrays in drug discovery and basic research,' Anal Bioanal Chem., vol.377, pp.486-495, 2003   DOI
5 Litke AM, Chichilnisky EJ, Dabrowski W, Grillo AA, Grybos P, Kashiguine S, Rahman M, Taylor G, 'Large-scale imaging of reginal output activity,' Nuclear Instruments and Methods in Physics Research, vol. A 501, pp.298-307, 2003
6 Egert U, Knott T, Schwarz C, Nawrot M, Brandt A, Rotter S, Diesmann M, 'MEA-Tools: an open source toolbox for the analysis of multi-electrode data with MATLAB,' J Neurosci. Methods, vol. 117, pp. 33-42, 2002   DOI   ScienceOn
7 De Ryck M, Reempts JV, Borgers M, Wauquier A, Janssen PAJ, 'Photochemical stroke model; flunarizine prevents sensorimotor deficits after neocortical infarts in rats,' Stroke, vol. 20, pp.1383-1390,1989   DOI   ScienceOn
8 Shimono K, Baudry M, Panchenko V, Taketani M, 'Chronic multichannel recordings from organotypic hippocampal slice cultures: protection from excitotoxic effects of NMDA by non-competitive NMDA antagonists,' J Neurosci. Methods, vol. 120, pp.193-202, 2002   DOI   ScienceOn
9 Steidl E-M, Neveu E, Bertrand D, Buisson B, 'The adult rat hippocampal slice revisited with multi-electrode arrays,' Brain Res., vol. 22, pp.70-84, 2006
10 Haynes LE, Griffiths MR, Hyde RE, Barber DJ, Mitchell IJ, 'Dexamethasone induces limited apoptosis and extensive sublethal damage to specific subregions of the striatum and hippocampus: implications for mood disorders,' J. Neurosci., vol. 104, pp.57-69, 2001   DOI   ScienceOn
11 Egert U, Schlosshauer B, Fennrich S, Nisch W, Fejtl M, Knott T, M?ller T, H?mmerle H, 'A Novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays,' Brain Research Protocol, vol. 2, pp.229-242, 1998   DOI   ScienceOn
12 Kandel ER, Schwartz JH, Jessell TM, Principles of neural science (4th), New York, USA, McGraw-Hill, 2000
13 Ferrer I, Lopez E, Blanco R, Rivera R, Ballabriga J, Pozas E, Marti E, 'Bcl-2, Bax, and Bcl-x expression in the CAl area of the hippocampus following transient forbrain ischemia in the adult gerbil,' Exp. Brain. Res., vol. 121, pp. 167-173, 1998   DOI
14 Oka H, Shimono K, Ogawa R, Sugihara H, Taketani M, 'A new planar multielectrode array for extracellular recording: application to hippocampal acute slice,' J Neurosci Methods, vol.93, pp.61-67, 1999   DOI   ScienceOn
15 Thomas CJ, Springer PA, Loeb GE, Berwald-Netter Y, Okun LM, 'A miniature microelectrode array to monitor the bioelectric activity of cultured cells,' Exp Cell Res., vol. 74, pp.61-66, 1972   DOI   ScienceOn