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http://dx.doi.org/10.9718/JBER.2006.27.5.218

Surface Immobilization of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ onto Biodegradable Polymer for Tissue Regeneration  

Lee, S.G. (Dept. of Biomedical Engineering, Inje Univ.)
Lee, J.B. (Dept. of Biomedical Engineering, Inje Univ.)
Yu, S.M. (Dept. of Biomedical Engineering, Inje Univ.)
Park, J.C. (Dept. Medical Eng., Collage of Med., Yonsei Univ.)
Choi, J.B. (Dept. Mechanical Systems Eng., Hansung Univ.)
Kim, J.K. (Dept. of Biomedical Engineering, Inje Univ.)
Publication Information
Journal of Biomedical Engineering Research / v.27, no.5, 2006 , pp. 218-223 More about this Journal
Abstract
We examined the effects of ${\beta}$-glucan-reinforced PLGA film and scaffold on HDFs (human dermal fibroblast) attachment and proliferation. The PLGA films were prepared by simple solvent-casting method. The prepared films were grafted with $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in various ratios after plasma treatment on surface. The surface of the film was characterized by contact angle measurement, scanning electron microscope (SEM), and Fourier-transform infrared spectrophotometer (FT-IR). The amount of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in the prepared film was indirectly determined by phenol-sulfuric acid method. The HDFs (Human dermal fibroblasts) were used to evaluate the cell attachment and proliferation on PLGA specimens before and after plasma/${\beta}-glucan$ treatment. The result showed that the plasma treated groups exhibited more mont of ${\beta}-glucan$ might be grafted than the non plasma treated groups. Cell attachment was significantly enhanced in the plasma/${\beta}-glucan$ grafted group after 4 hours incubation (p<0.05) due to the improved hydrophilicity and cytoactivity effect of the ${\beta}-glucan$. The cell proliferation of plasma/${\beta}-glucan$ (2mg/ml) grafted group was the highest rate among the groups (p<0.05).
Keywords
tissue regeneration; PLGA; ${\beta}-glucan$; plasma treatment; cell affinity;
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