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A Study on the Effect of Physical Stimuli on Bone Cell Differentiation Using a Hybrid Bioreactor  

이창양 (한국과학기술연구원 의과학연구센터, 고려대학교 공과대학 기계공학과)
최귀원 (한국과학기술연구원 의과학연구센)
홍대희 (고려대학교 공과대학 기계공학과)
Publication Information
Journal of Biomedical Engineering Research / v.25, no.4, 2004 , pp. 309-314 More about this Journal
Abstract
in this study, hybrid bioreactor was used to apply physical stimuli in cell culture. Effect of the applied physical stimuli on the growth and differentiation of MC3T3-El cell in a three-dimensional Chitosan scaffold were studied by using the hybrid bioreactor. The hybrid bioreactor for physical stimulus was specially designed to apply uniaxial cyclic compressive and shear strain. Physical stimulus was applied over a period of 14 days with 150 cycles per day at a frequency of 0.5Hz. Strain magnitude was 2.5% of the scaffold size. Control group and physically stimulated group of the MC3T3-El tell were incubated and harvested at the indicated times (Day 6, 8, 10, 12, 14). The total amount of protein, which obtained information of cell growth, was determined by Lowey method. Alkaline phosphatase activity was examined by ELISA. Physically stimulated group using the hybrid bioreactor was increased in alkaline phosphatase activity comparing with control group. The nodule formation and calcium deposit of the physical stimuli group which resulted in cell differentiation was faster than that of control group.
Keywords
MC3T3-El; Bioreactor; Physical stimuli; Proliferation; Differentiation; Alkaline phosphatase activity;
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