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http://dx.doi.org/10.4062/biomolther.2022.103

Immune Responses to Plant-Derived Recombinant Colorectal Cancer Glycoprotein EpCAM-FcK Fusion Protein in Mice  

Lim, Chae-Yeon (Department of Medicine, College of Medicine, Chung-Ang University)
Kim, Deuk-Su (Department of Medicine, College of Medicine, Chung-Ang University)
Kang, Yangjoo (Department of Medicine, College of Medicine, Chung-Ang University)
Lee, Ye-Rin (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Kibum (Department of Medicine, College of Medicine, Chung-Ang University)
Kim, Do Sun (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Moon-Soo (Department of Chemistry, Ogden College of Science and Engineering, Western Kentucky University)
Ko, Kisung (Department of Medicine, College of Medicine, Chung-Ang University)
Publication Information
Biomolecules & Therapeutics / v.30, no.6, 2022 , pp. 546-552 More about this Journal
Abstract
Epidermal cell adhesion molecule (EpCAM) is a tumor-associated antigen (TAA), which has been considered as a cancer vaccine candidate. The EpCAM protein fused to the fragment crystallizable region of immunoglobulin G (IgG) tagged with KDEL endoplasmic reticulum (ER) retention signal (EpCAM-FcK) has been successfully expressed in transgenic tobacco (Nicotiana tabacum cv. Xanthi) and purified from the plant leaf. In this study, we investigated the ability of the plant-derived EpCAM-FcK (EpCAM-FcKP) to elicit an immune response in vivo. The animal group injected with the EpCAM-FcKP showed a higher differentiated germinal center (GC) B cell population (~9%) compared with the animal group injected with the recombinant rhEpCAM-Fc chimera (EpCAM-FcM). The animal group injected with EpCAM-FcKP (~42%) had more differentiated T follicular helper cells (Tfh) than the animal group injected with EpCAM-FcM (~7%). This study demonstrated that the plant-derived EpCAM-FcK fusion antigenic protein induced a humoral immune response in mice.
Keywords
Colorectal cancer; EpCAM; Germinal center B cells; Transgenic plants;
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