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http://dx.doi.org/10.4062/biomolther.2020.090

Diclofenac Inhibits Phorbol Ester-Induced Gene Expression and Production of MUC5AC Mucin via Affecting Degradation of IkBα and Translocation of NF-kB p65 in NCI-H292 Cells  

Jin, Fengri (Department of Pharmacology, School of Medicine, Chungnam National University)
Li, Xin (Department of Pharmacology, School of Medicine, Chungnam National University)
Lee, Hyun Jae (Smith Liberal Arts College and Department of Addiction Science, Graduate School, Sahmyook University)
Lee, Choong Jae (Department of Pharmacology, School of Medicine, Chungnam National University)
Publication Information
Biomolecules & Therapeutics / v.28, no.5, 2020 , pp. 431-436 More about this Journal
Abstract
In this study, diclofenac, a non-steroidal anti-inflammatory drug, was investigated for its potential effect on the gene expression and production of airway MUC5AC mucin. The human respiratory epithelial NCI-H292 cells were pretreated with diclofenac for 30 min and stimulated with phorbol 12-myristate 13-acetate (PMA), for the following 24 h. The effect of diclofenac on PMA-induced nuclear factor kappa B (NF-kB) signaling pathway was also investigated. Diclofenac suppressed the production and gene expression of MUC5AC mucins, induced by PMA through the inhibition of degradation of inhibitory kappa Bα (IkBα) and NF-kB p65 nuclear translocation. These results suggest diclofenac regulates the gene expression and production of mucin through regulation of NF-kB signaling pathway, in human airway epithelial cells.
Keywords
MUC5AC; Pulmonary mucin; Diclofenac;
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