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7,8,4'-Trihydroxyisoflavone, a Metabolized Product of Daidzein, Attenuates 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells  

Ko, Yong-Hyun (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Kim, Seon-Kyung (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Kwon, Seung-Hwan (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Seo, Jee-Yeon (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Lee, Bo-Ram (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Kim, Young-Jung (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Hur, Kwang-Hyun (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Kim, Sun Yeou (College of Pharmacy, Gachon University)
Lee, Seok-Yong (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Jang, Choon-Gon (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Publication Information
Biomolecules & Therapeutics / v.27, no.4, 2019 , pp. 363-372 More about this Journal
Daidzein isolated from soybean (Glycine max) has been widely studied for its antioxidant and anti-inflammatory activities. However, the protective effects of 7,8,4'-trihydroxyisoflavone (THIF), a major metabolite of daidzein, on 6-hydroxydopamine (OHDA)-induced neurotoxicity are not well understood. In the current study, 7,8,4'-THIF significantly inhibited neuronal cell death and lactate dehydrogenase (LDH) release induced by 6-OHDA in SH-SY5Y cells, which were used as an in vitro model of Parkinson's disease (PD). Moreover, pretreatment with 7,8,4'-THIF significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) and decreased malondialdehyde (MDA) activity in 6-OHDA-induced SH-SY5Y cells. In addition, 7,8,4'-THIF significantly recovered 6-OHDA-induced cleaved caspase-3, cleaved caspase-9, cleaved poly-ADP-ribose polymerase (PARP), increased Bax, and decreased Bcl-2 levels. Additionally, 7,8,4'-THIF significantly restored the expression levels of phosphorylated c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), phosphatidylinositol 3-kinases (PI3K)/Akt, and glycogen synthase kinase-3 beta ($GSK-3{\beta}$) in 6-OHDA-induced SH-SY5Y cells. Further, 7,8,4'-THIF significantly increased the reduced tyrosine hydroxylase (TH) level induced by 6-OHDA in SH-SY5Y cells. Collectively, these results suggest that 7,8,4'-THIF protects against 6-OHDA-induced neuronal cell death in cellular PD models. Also, these effects are mediated partly by inhibiting activation of the MAPK and PI3K/Akt/$GSK-3{\beta}$ pathways.
7,8,4'-Trihydroxyisoflavone; 6-Hydroxydopamine; Neurotoxicity; Apoptosis; Parkinson's disease;
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