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Comparative Behavioral Correlation of High and Low-Performing Mice in the Forced Swim Test  

Valencia, Schley (Department of Neuroscience, School of Medicine, Konkuk University)
Gonzales, Edson Luck (Department of Neuroscience, School of Medicine, Konkuk University)
Adil, Keremkleroo Jym (Department of Neuroscience, School of Medicine, Konkuk University)
Jeon, Se Jin (Center for Neuroscience, Korea Institute of Science and Technology)
Kwon, Kyoung Ja (Department of Neuroscience, School of Medicine, Konkuk University)
Cho, Kyu Suk (Department of Neuroscience, School of Medicine, Konkuk University)
Shin, Chan Young (Department of Neuroscience, School of Medicine, Konkuk University)
Publication Information
Biomolecules & Therapeutics / v.27, no.4, 2019 , pp. 349-356 More about this Journal
Behavioral analysis in mice provided important contributions in helping understand and treat numerous neurobehavioral and neuropsychiatric disorders. The behavioral performance of animals and humans is widely different among individuals but the neurobehavioral mechanism of the innate difference is seldom investigated. Many neurologic conditions share comorbid symptoms that may have common pathophysiology and therapeutic strategy. The forced swim test (FST) has been commonly used to evaluate the "antidepressant" properties of drugs yet the individual difference analysis of this test was left scantly investigated along with the possible connection among other behavioral domains. This study conducted an FST-screening in outbred CD-1 male mice and segregated them into three groups: high performers (HP) or the active swimmers, middle performers (MP), and low performers (LP) or floaters. After which, a series of behavioral experiments were performed to measure their behavioral responses in the open field, elevated plus maze, Y maze, three-chamber social assay, novel object recognition, delay discounting task, and cliff avoidance reaction. The behavioral tests battery revealed that the three groups displayed seemingly correlated differences in locomotor activity and novel object recognition but not in other behaviors. This study suggests that the HP group in FST has higher locomotor activity and novelty-seeking tendencies compared to the other groups. These results may have important implications in creating behavior database in animal models that could be used for predicting interconnections of various behavioral domains, which eventually helps to understand the neurobiological mechanism controlling the behaviors in individual subjects.
Forced swim test; Immobility; Locomotor activity; Object exploration; Novelty-seeking;
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1 Baxter, M. G. (2010) "I've seen it all before": explaining age-related impairments in object recognition. Theoretical comment on Burke et al. (2010). Behav. Neurosci. 124, 706-709.   DOI
2 Bogdanova, O. V., Kanekar, S., D'Anci, K. E. and Renshaw, P. F. (2013) Factors influencing behavior in the forced swim test. Physiol. Behav. 118, 227-239.   DOI
3 Can, A., Dao, D. T., Arad, M., Terrillion, C. E., Piantadosi, S. C. and Gould, T. D. (2012) The mouse forced swim test. J. Vis. Exp. (59), 3638.
4 Chen, X., Zhang, W., Li, T., Guo, Y., Tian, Y., Wang, F., Liu, S., Shen, H.-Y., Feng, Y. and Xiao, L. (2015) Impairment of oligodendroglia maturation leads to aberrantly increased cortical glutamate and anxiety-like behaviors in juvenile mice. Front. Cell. Neurosci. 9, 467.
5 Cloninger, C. R. (1986) A unified biosocial theory of personality and its role in the development of anxiety states. Psych. Dev. 3, 167-226.
6 Commons, K. G., Cholanians, A. B., Babb, J. A. and Ehlinger, D. G. (2017) The rodent forced swim test measures stress-coping strategy, not depression-like behavior. ACS Chem. Neurosci. 8, 955-960.   DOI
7 Crawley, J. N. (2007) What's Wrong with My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice. John Wiley & Sons.
8 dela Pena, I., Gonzales, E. L., de la Pena, J. B., Kim, B.-N., Han, D. H., Shin, C. Y. and Cheong, J. H. (2015) Individual differences in novelty-seeking behavior in spontaneously hypertensive rats: Enhanced sensitivity to the reinforcing effect of methylphenidate in the high novelty-preferring subpopulation. J. Neurosci. Methods 252, 48-54.   DOI
9 Ennaceur, A. (2010) One-trial object recognition in rats and mice: methodological and theoretical issues. Behav. Brain Res. 215, 244-254.   DOI
10 Gonzales, E. L. T., Jang, J.-H., Mabunga, D. F. N., Kim, J.-W., Ko, M. J., Cho, K. S., Bahn, G. H., Hong, M., Ryu, J. H. and Kim, H. J. (2016) Supplementation of Korean Red Ginseng improves behavior deviations in animal models of autism. Food Nutr. Res. 60, 29245.   DOI
11 Matta, A. d., Goncalves, F. L. and Bizarro, L. (2012) Delay discounting: concepts and measures. Psychol. Neurosci. 5, 135-146.   DOI
12 Hooks, M. S., Colvin, A. C., Juncos, J. L. and Justice, J. B., Jr. (1992) Individual differences in basal and cocaine-stimulated extracellular dopamine in the nucleus accumbens using quantitative microdialysis. Brain Res. 587, 306-312.   DOI
13 Jama, A., Cecchi, M., Calvo, N., Watson, S. and Akil, H. (2008) Interindividual differences in novelty-seeking behavior in rats predict differential responses to desipramine in the forced swim test. Psychopharmacology 198, 333-340.   DOI
14 Karl, T., Pabst, R. and von Horsten, S. (2003) Behavioral phenotyping of mice in pharmacological and toxicological research. Exp. Toxicol. Pathol. 55, 69-83.   DOI
15 Kim, K. C., Kim, P., Go, H. S., Choi, C. S., Yang, S.-I., Cheong, J. H., Shin, C. Y. and Ko, K. H. (2011) The critical period of valproate exposure to induce autistic symptoms in Sprague-Dawley rats. Toxicol. Lett. 201, 137-142.   DOI
16 Lezak, K. R., Missig, G. and Carlezon, W. A., Jr. (2017) Behavioral methods to study anxiety in rodents. Dialogues Clin. Neurosci. 19, 181.   DOI
17 Mitchell, S. H. (2014) Assessing delay discounting in mice. Curr. Protoc. Neurosci. 66, 8-30.   DOI
18 Moy, S., Nadler, J., Perez, A., Barbaro, R., Johns, J., Magnuson, T., Piven, J. and Crawley, J. (2004) Sociability and preference for social novelty in five inbred strains: an approach to assess autisticlike behavior in mice. Genes. Brain Behav. 3, 287-302.   DOI
19 Pellow, S., Chopin, P., File, S. E. and Briley, M. (1985) Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J. Neurosci. Methods 14, 149-167.   DOI
20 National Research Council (2010) Guide for the Care and Use of Laboratory Animals. National Academies Press.
21 Petit-Demouliere, B., Chenu, F. and Bourin, M. (2005) Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology 177, 245-255.   DOI
22 Sarter, M., Bodewitz, G. and Stephens, D. N. (1988) Attenuation of scopolamine-induced impairment of spontaneous alternation behaviour by antagonist but not inverse agonist and agonist ${\beta}$-carbolines. Psychopharmacology 94, 491-495.   DOI
23 Pitychoutis, P. M., Pallis, E. G., Mikail, H. G. and Papadopoulou-Daifoti, Z. (2011) Individual differences in novelty-seeking predict differential responses to chronic antidepressant treatment through sex-and phenotype-dependent neurochemical signatures. Behav. Brain Res. 223, 154-168.   DOI
24 Prut, L. and Belzung, C. (2003) The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur. J. Pharmacol. 463, 3-33.   DOI
25 Rosenzweig, M. R., Breedlove, S. M. and Leiman, A. L. (2002) Biological Psychology: an Introduction to Behavioral, Cognitive, and Clinical Neuroscience. Sinauer Associates.
26 Stafstrom, C. E. (2006) Behavioral and cognitive testing procedures in animal models of epilepsy. In Models of Seizures and Epilepsy. Elsevier Inc.
27 Stedenfeld, K. A., Clinton, S. M., Kerman, I. A., Akil, H., Watson, S. J. and Sved, A. F. (2011) Novelty-seeking behavior predicts vulnerability in a rodent model of depression. Physiol. Behav. 103, 210-216.   DOI
28 Verheij, M. M., de Mulder, E. L., De Leonibus, E., van Loo, K. M. and Cools, A. R. (2008) Rats that differentially respond to cocaine differ in their dopaminergic storage capacity of the nucleus accumbens. J. Neurochem. 105, 2122-2133.   DOI
29 Stults-Kolehmainen, M. A. and Sinha, R. (2014) The effects of stress on physical activity and exercise. Sports Med. 44, 81-121.   DOI
30 Tanaka, J. W. and Curran, T. (2001) A neural basis for expert object recognition. Psychol. Sci. 12, 43-47.   DOI
31 Wolf, A., Bauer, B., Abner, E. L., Ashkenazy-Frolinger, T. and Hartz, A. M. (2016) A comprehensive behavioral test battery to assess learning and memory in 129S6/Tg2576 mice. PLoS ONE 11, e0147733.   DOI
32 Vogel-Ciernia, A. and Wood, M. A. (2014) Examining object location and object recognition memory in mice. Curr. Protoc. Neurosci. 69, 8.31.1-8.31.17.
33 Walf, A. A. and Frye, C. A. (2007) The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat. Protoc. 2, 322-328.   DOI
34 Wermelinger Avila, M. P., Correa, J. C., Lucchetti, A. L. G. and Lucchetti, G. (2018) The role of physical activity in the association between resilience and mental health in older adults. J. Aging Phys. Act. 26, 248-253.   DOI
35 Yoshida, S., Numachi, Y., Matsuoka, H. and Sato, M. (1998) Impairment of cliff avoidance reaction induced by subchronic methamphetamine administration and restraint stress: comparison between two inbred strains of rats. Prog. Neuropsychopharmacol. Biol. Psychiatry 22, 1023-1032.   DOI