Browse > Article
http://dx.doi.org/10.4062/biomolther.2018.049

Cytoprotective Effect of Taurine against Hydrogen Peroxide-Induced Oxidative Stress in UMR-106 Cells through the Wnt/β-Catenin Signaling Pathway  

Lou, Jing (Department of Immunology, Jinzhou Medical University)
Han, Donghe (Department of Neurobiology, Jinzhou Medical University)
Yu, Huihui (Department of Immunology, Jinzhou Medical University)
Yu, Guang (Department of Immunology, Jinzhou Medical University)
Jin, Meihua (Department of Immunology, Jinzhou Medical University)
Kim, Sung-Jin (Departments of Pharmacology and Toxicology, Metabolic Diseases Research Laboratory, School of Dentistry, KyungHee University)
Publication Information
Biomolecules & Therapeutics / v.26, no.6, 2018 , pp. 584-590 More about this Journal
Abstract
Osteoporosis development is closely associated with oxidative stress and reactive oxygen species (ROS). Taurine has potential antioxidant effects, but its role in osteoblasts is not clearly understood. The aim of this study was to determine the protective effects and mechanisms of actions of taurine on hydrogen peroxide ($H_2O_2$)-induced oxidative stress in osteoblast cells. UMR-106 cells were treated with taurine prior to $H_2O_2$ exposure. After treatment, cell viability, apoptosis, intracellular ROS production, malondialdehyde content, and alkaline phosphate (ALP) activity were measured. We also investigated the protein levels of ${\beta}-catenin$, ERK, CHOP and NF-E2-related factor 2 (Nrf2) along with the mRNA levels of Nrf2 downstream antioxidants. The results showed that pretreatment of taurine could reverse the inhibition of cell viability and suppress the induced apoptosis in a dose-dependent manner: taurine significantly reduced $H_2O_2$-induced oxidative damage and expression of CHOP, while it induced protein expression of Nrf2 and ${\beta}-catenin$ and activated ERK phosphorylation. DKK1, a Wnt/${\beta}-catenin$ signaling inhibitor, significantly suppressed the taurine-induced Nrf2 signaling pathway and increased CHOP. Activation of ERK signaling mediated by taurine in the presence of $H_2O_2$ was significantly inhibited by DKK1. These data demonstrated that taurine protects osteoblast cells against oxidative damage via Wnt/${\beta}-catenin$-mediated activation of the ERK signaling pathway.
Keywords
Taurine; Oxidative stress; Antioxidants; Wnt/${\beta}-catenin$; Osteoblast;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Abdal Dayem, A., Hossain, M. K., Lee, S. B., Kim, K., Saha, S. K., Yang, G. M., Choi, H. Y. and Cho, S. G. (2017) The Role of Reactive Oxygen Species (ROS) in the biological activities of metallic nanoparticles. Int. J. Mol. Sci. 18, E120.   DOI
2 Ahn, J., Lee, H., Kim, S. and Ha, T. (2010) Curcumin-induced suppression of adipogenic differentiation is accompanied by activation of $$Wnt/{\beta}$$-catenin signaling. Am. J. Physiol. Cell Physiol. 298, C1510-C1516.   DOI
3 Bartell, S. M., Kim, H. N., Ambrogini, E., Han, L., Iyer, S., Serra Ucer, S., Rabinovitch, P., Jilka, R. L., Weinstein, R. S., Zhao, H., O'Brien, C. A., Manolagas, S. C. and Almeida, M. (2014) FoxO proteins restrain osteoclastogenesis and bone resorption by attenuating H2O2 accumulation. Nat. Commun. 5, 3773.   DOI
4 Son, H. Y., Kim, H. and Y, H. K. (2007) Taurine prevents oxidative damage of high glucose-induced cataractogenesis in isolated rat lenses. J. Nutr. Sci. Vitaminol. (Tokyo) 53, 324-330.   DOI
5 Suzuki, T. and Yamamoto, M. (2015) Molecular basis of the Keap1-Nrf2 system. Free Radic. Biol. Med. 88, 93-100.   DOI
6 Tapia, J. C., Torres, V. A., Rodriguez, D. A., Leyton, L. and Quest, A. F. (2006) Casein kinase 2 (CK2) increases survivin expression via enhanced beta-catenin-T cell factor/lymphoid enhancer binding factor-dependent transcription. Proc. Natl. Acad. Sci. U.S.A. 103, 15079-15084.   DOI
7 Wauquier, F., Leotoing, L., Coxam, V., Guicheux, J. and Wittrant, Y. (2009) Oxidative stress in bone remodelling and disease. Trends Mol. Med. 15, 468-477.   DOI
8 Chen, J. R., Lazarenko, O. P., Wu, X., Kang, J., Blackburn, M. L., Shankar, K., Badger, T. M. and Ronis, M. J. (2010) Dietary-induced serum phenolic acids promote bone growth via p38 MAPK/betacatenin canonical Wnt signaling. J. Bone Miner. Res. 25, 2399-2411.   DOI
9 Bernardo, I., Bozinovski, S. and Vlahos, R. (2015) Targeting oxidantdependent mechanisms for the treatment of COPD and its comorbidities. Pharmacol. Ther. 155, 60-79.   DOI
10 Cervellati, C. and Bergamini, C. M. (2016) Oxidative damage and the pathogenesis of menopause related disturbances and diseases. Clin. Chem. Lab. Med. 54, 739-753.
11 Zhang, L.-Y., Zhou, Y.-Y., Chen, F., Wang, B., Li, J., Deng, Y.-W., Liu, W. D., Wang, Z. G., Li, Y. W., Li, D. Z., Lv, G. H. and Yin, B.-L. (2011) Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway. Braz. J. Med. Biol. Res. 44, 618-623.   DOI
12 Wong, S. Y., Tan, M. G., Wong, P. T., Herr, D. R. and Lai, M. K. (2016) Andrographolide induces Nrf2 and heme oxygenase 1 in astrocytes by activating p38 MAPK and ERK. J. Neuroinflammation 13, 251.   DOI
13 Yang, Y., Zhang, Y., Wang, L. and Lee, S. (2017) Levistolide A induces apoptosis via ros-mediated ER stress pathway in colon cancer cells. Cell. Physiol. Biochem. 42, 929-938.   DOI
14 Yee, C. S., Manilay, J. O., Chang, J. C., Hum, N. R., Murugesh, D. K., Bajwa, J., Mendez, M. E., Economides, A. E., Horan, D. J., Robling, A. G. and Loots, G. G. (2018) Conditional Deletion of Sost in MSC-derived lineages Identifies Specific Cell Type Contributions to Bone Mass and B Cell Development. J. Bone Miner. Res. doi:10.1002/jbmr.3467 [Epub ahead of print].   DOI
15 Higuchi, M., Celino, F. T., Shimizu-Yamaguchi, S., Miura, C. and Miura, T. (2012) Taurine plays an important role in the protection of spermatogonia from oxidative stress. Amino Acids 43, 2359-2369.   DOI
16 Cheung, K. L., Lee, J. H., Shu, L., Kim, J. H., Sacks, D. B. and Kong, A. N. (2013) The ras GTPase-activating-like protein IQGAP1 mediates Nrf2 protein activation via the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) kinase (MEK)-ERK pathway. J. Biol. Chem. 288, 22378-22386.   DOI
17 Dai, P., Mao, Y., Sun, X., Li, X., Muhammad, I., Gu, W., Zhang, D., Zhou, Y., Ni, Z., Ma, J. and Huang, S. (2017) Attenuation of oxidative stress-induced osteoblast apoptosis by curcumin is associated with preservation of mitochondrial functions and increased Akt-GSK3beta signaling. Cell. Physiol. Biochem. 41, 661-677.   DOI
18 Greenblatt, M. B., Shim, J. H., Zou, W., Sitara, D., Schweitzer, M., Hu, D., Lotinun, S., Sano, Y., Baron, R., Park, J. M., Arthur, S., Xie, M., Schneider, M. D., Zhai, B., Gygi, S., Davis, R. and Glimcher, L. H. (2010) The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice. J. Clin. Invest. 120, 2457-2473.   DOI
19 Zou, W., Greenblatt, M. B., Shim, J. H., Kant, S., Zhai, B., Lotinun, S., Brady, N., Hu, D. Z., Gygi, S. P., Baron, R., Davis, R. J., Jones, D. and Glimcher, L. H. (2011) MLK3 regulates bone development downstream of the faciogenital dysplasia protein FGD1 in mice. J. Clin. Invest. 121, 4383-4392.   DOI
20 Han, D., Chen, W., Gu, X., Shan, R., Zou, J., Liu, G., Shahid, M., Gao, J. and Han, B. (2017) Cytoprotective effect of chlorogenic acid against hydrogen peroxide-induced oxidative stress in MC3T3-E1 cells through PI3K/Akt-mediated Nrf2/HO-1 signaling pathway. Oncotarget 8, 14680-14692.
21 Hu, D., Bi, X., Fang, W., Han, A. and Yang, W. (2009) GSK3b is involved in JNK2-mediated b-catenin inhibition. PLoS ONE 4, e6640.   DOI
22 Jang, H. J., Hong, E. M., Kim, M., Kim, J. H., Jang, J., Park, S. W., Byun, H. W., Koh, D. H., Choi, M. H., Kae, S. H. and Lee, J. (2016) Simvastatin induces heme oxygenase-1 via NF-E2-related factor 2 (Nrf2) activation through ERK and PI3K/Akt pathway in colon cancer. Oncotarget 7, 46219-46229.
23 Lin, P., Tian, X. H., Yi, Y. S., Jiang, W. S., Zhou, Y. J. and Cheng, W. J. (2015) Luteolin-induced protection of H(2)O(2)-induced apoptosis in PC12 cells and the associated pathway. Mol. Med. Rep. 12, 7699-7704.   DOI
24 Jang, H. J. and Kim, S. J. (2013) Taurine exerts anti-osteoclastogenesis activity via inhibiting ROS generation, JNK phosphorylation and COX-2 expression in RAW264.7 cells. J. Recept. Signal Transduct. Res. 33, 387-391.   DOI
25 Kim, H. Y., Yoon, J. Y., Yun, J. H., Cho, K. W., Lee, S. H., Rhee, Y. M., Jung, H. S., Lim, H. J., Lee, H., Choi, J., Heo, J. N., Lee, W., No, K. T., Min, D. and Choi, K. Y. (2015) CXXC5 is a negative-feedback regulator of the Wnt/beta-catenin pathway involved in osteoblast differentiation. Cell Death Differ. 22, 912-920.   DOI
26 Koizumi, Y., Nagai, K., Gao, L., Koyota, S., Yamaguchi, T., Natsui, M., Imai, Y., Hasumi, K., Sugiyama, T. and Kuba, K. (2018) Involvement of RSK1 activation in malformin-enhanced cellular fibrinolytic activity. Sci. Rep. 8, 5472.   DOI
27 Lee, H. S., Lee, G. S., Kim, S. H., Kim, H. K., Suk, D. H. and Lee, D. S. (2014) Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway. BMB Rep. 47, 98-103.   DOI
28 Li, S. T., Chen, N. N., Qiao, Y. B., Zhu, W. L., Ruan, J. W. and Zhou, X. Z. (2016) SC79 rescues osteoblasts from dexamethasone though activating Akt-Nrf2 signaling. Biochem. Biophys. Res. Commun. 479, 54-60.   DOI
29 Liu, W. D., Mao, L., Ji, F., Chen, F. L., Hao, Y. D. and Liu, G. (2017) Targeted activation of AMPK by GSK621 ameliorates H2O2-induced damages in osteoblasts. Oncotarget 8, 10543-10552.
30 Matsushita, T., Chan, Y. Y., Kawanami, A., Balmes, G., Landreth, G. E. and Murakami, S. (2009) Extracellular signal-regulated kinase 1 (ERK1) and ERK2 play essential roles in osteoblast differentiation and in supporting osteoclastogenesis. Mol. Cell. Biol. 29, 5843-5857.   DOI
31 Nguyen, T., Nioi, P. and Pickett, C. B. (2009) The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress. J. Biol. Chem. 284, 13291-13295.   DOI
32 Nusse, R. and Clevers, H. (2017) Wnt/beta-catenin signaling, disease, and emerging therapeutic modalities. Cell 169, 985-999.   DOI
33 Pisoschi, A. M. and Pop, A. (2015) The role of antioxidants in the chemistry of oxidative stress: a review. Eur. J. Med. Chem. 97, 55-74.   DOI
34 Qiao, X., Nie, Y., Ma, Y., Chen, Y., Cheng, R., Yin, W., Hu, Y., Xu, W. and Xu, L. (2016) Irisin promotes osteoblast proliferation and differentiation via activating the MAP kinase signaling pathways. Sci. Rep. 6, 18732.   DOI
35 Soares-Silva, M., Diniz, F. F., Gomes, G. N. and Bahia, D. (2016) The mitogen-activated protein kinase (MAPK) pathway: role in immune evasion by trypanosomatids. Front. Microbiol. 7, 183.
36 Roman-Garcia, P., Quiros-Gonzalez, I., Mottram, L., Lieben, L., Sharan, K., Wangwiwatsin, A., Tubio, J., Lewis, K., Wilkinson, D., Santhanam, B., Sarper, N., Clare, S., Vassiliou, G. S., Velagapudi, V. R., Dougan, G. and Yadav, V. K. (2014) Vitamin B(1)(2)-dependent taurine synthesis regulates growth and bone mass. J. Clin. Invest. 124, 2988-3002.   DOI
37 Shim, J. H., Greenblatt, M. B., Zou, W., Huang, Z., Wein, M. N., Brady, N., Hu, D., Charron, J., Brodkin, H. R., Petsko, G. A., Zaller, D., Zhai, B., Gygi, S., Glimcher, L. H. and Jones, D. C. (2013) Schnurri-3 regulates ERK downstream of WNT signaling in osteoblasts. J. Clin. Invest. 123, 4010-4022.   DOI