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http://dx.doi.org/10.4062/biomolther.2016.156

Sedative Effect of Sophora flavescens and Matrine  

Lee, Hyun-ju (Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University)
Lee, Sun-young (Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University)
Jang, Daehyuk (Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University)
Chung, Sun-Yong (Department of Oriental Neuropsychiatry, Kyung Hee University Korean Medicine Hospital at Gangdong)
Shim, Insop (Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University)
Publication Information
Biomolecules & Therapeutics / v.25, no.4, 2017 , pp. 390-395 More about this Journal
Abstract
The present study investigated the sedative effects of Sophora flavescens (SF) and its bioactive compound, matrine through performing locomotor activity test and the electroencephalography (EEG) analysis in the rat. The underlying neural mechanism of their beneficial effects was determined by assessing c-Fos immunoreactivity and serotonin (5-HT) in the brain utilizing immunohistochemical method and enzyme-linked immunosorbent assay. The results showed that SF and matrine administration had an effect on normalization of caffeine-induced hyperactivity and promoting a shift toward non-rapid eye movement (NREM) sleep. c-Fos-immunoreactivity and 5-HT level in the ventrolateral preoptic nucleus (VLPO), a sleep promoting region, were increased in the both SF and matrine-injected groups. In conclusion, SF and its bioactive compound, matrine alleviated caffeine-induced hyperactivity and promoted NREM sleep by activating VLPO neurons and modulating serotonergic transmission. It is suggested that SF might be a useful natural alternatives for hypnotic medicine.
Keywords
Sophora flavescens; Matrine; Serotonin; Electroencephalography; Locomotor; Sedative effect;
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1 Paterson, L. M., Wilson, S. J., Nutt, D. J., Hutson, P. H. and Ivarsson, M. (2007) A translational, caffeine-induced model of onset insomnia in rats and healthy volunteers. Psychopharmacology (Berl.) 191, 943-950.   DOI
2 Paxinos, G., Watson, C., Pennisi, M. and Topple, A. (1985) Bregma, lambda and the interaural midpoint in stereotaxic surgery with rats of different sex, strain and weight. J. Neurosci. Methods 13, 139-143.   DOI
3 Sangare, A., Dubourget, R., Geoffroy, H., Gallopin, T. and Rancillac, A. (2016) Serotonin differentially modulates excitatory and inhibitory synaptic inputs to putative sleep-promoting neurons of the ventrolateral preoptic nucleus. Neuropharmacology 109, 29-40.   DOI
4 Shao, H., Yang, B., Hu, R. and Wang, Y. (2013) Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-kappaB signaling pathway. Oncol. Lett. 6, 517-520.   DOI
5 Sherin, J. E., Shiromani, P. J., McCarley, R. W. and Saper, C. B. (1996) Activation of ventrolateral preoptic neurons during sleep. Science 271, 216-219.   DOI
6 Shin, D. H., Cha, Y. J., Joe, G. J., Yang, K. E., Jang, I. S., Kim, B. H. and Kim, J. M. (2013) Whitening effect of Sophora flavescens extract. Pharm. Biol. 51, 1467-1476.   DOI
7 Solarewicz, J. Z., Angoa-Perez, M., Kuhn, D. M. and Mateika, J. H. (2015) The sleep-wake cycle and motor activity, but not temperature, are disrupted over the light-dark cycle in mice genetically depleted of serotonin. Am. J. Physiol. Regul. Integr. Comp. Physiol. 308, R10-R17.   DOI
8 Tanabe, N., Kuboyama, T., Kazuma, K., Konno, K. and Tohda, C. (2016) The extract of roots of Sophora flavescens enhances the recovery of motor function by axonal growth in mice with a spinal cord injury. Front Pharmacol. 6, 326.
9 Alfajaro, M. M., Rho, M. C., Kim, H. J., Park, J. G., Kim, D. S., Hosmillo, M., Son, K. Y., Lee, J. H., Park, S. I., Kang, M. I., Ryu, Y. B., Park, K. H., Oh, H. M., Lee, S. W., Park, S. J., Lee, W. S. and Cho, K. O. (2014) Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Res. Vet. Sci. 96, 567-575.   DOI
10 Abdollahnejad, F., Mosaddegh, M., Kamalinejad, M., Mirnajafi-Zadeh, J., Najafi, F. and Faizi, M. (2016) Investigation of sedative and hypnotic effects of Amygdalus communis L. extract: behavioral assessments and EEG studies on rat. J. Nat. Med. 70, 190-197.   DOI
11 Bjorvatn, B., Gronli, J., Hamre, F., Sorensen, E., Fiske, E., Bjorkum, A. A., Portas, C. M. and Ursin, R. (2002) Effects of sleep deprivation on extracellular serotonin in hippocampus and frontal cortex of the rat. Neuroscience 113, 323-330.   DOI
12 Irwin, M. R., Olmstead, R. and Carroll, J. E. (2016) Sleep disturbance, sleep duration, and inflammation: A systematic review and metaanalysis of cohort studies and experimental sleep deprivation. Biol. Psychiatry 80, 40-52.   DOI
13 Whitney, M. S., Shemery, A. M., Yaw, A. M., Donovan, L. J., Glass, J. D. and Deneris, E. S. (2016) Adult brain serotonin deficiency causes hyperactivity, circadian disruption, and elimination of siestas. J. Neurosci. 36, 9828-9842.   DOI
14 Yanju, B., Yang, L., Hua, B., Hou, W., Shi, Z., Li, W., Li, C., Chen, C., Liu, R., Qin, Y. and Lv, W. (2014) A systematic review and metaanalysis on the use of traditional Chinese medicine compound kushen injection for bone cancer pain. Support. Care Cancer 22, 825-836.   DOI
15 Bravo, R., Matito, S., Cubero, J., Paredes, S. D., Franco, L., Rivero, M., Rodriguez, A. B. and Barriga, C. (2013) Tryptophan-enriched cereal intake improves nocturnal sleep, melatonin, serotonin, and total antioxidant capacity levels and mood in elderly humans. Age (Dordr.) 35, 1277-1285.   DOI
16 Ferre, S. (2008) An update on the mechanisms of the psychostimulant effects of caffeine. J. Neurochem. 105, 1067-1079.   DOI
17 Hajiaghaee, R., Faizi, M., Shahmohammadi, Z., Abdollahnejad, F., Naghdibadi, H., Najafi, F. and Razmi, A. (2016) Hydroalcoholic extract of Myrtus communis can alter anxiety and sleep parameters: a behavioural and EEG sleep pattern study in mice and rats. Pharm. Biol. 54, 2141-2148.   DOI
18 He, X., Fang, J., Huang, L., Wang, J. and Huang, X. (2015) Sophora flavescens Ait.: Traditional usage, phytochemistry and pharmacology of an important traditional Chinese medicine. J. Ethnopharmacol. 172, 10-29.   DOI
19 Irwin, M. R. (2015) Why sleep is important for health: a psychoneuroimmunology perspective. Annu. Rev. Psychol. 66, 143-172.   DOI
20 Klein, N., Gazola, A. C., de Lima, T. C., Schenkel, E., Nieber, K. and Butterweck, V. (2014) Assessment of sedative effects of Passiflora edulis f. flavicarpa and Passiflora alata extracts in mice, measured by telemetry. Phytother. Res. 28, 706-713.   DOI
21 Kroll, D. S., Nieva, H. R., Barsky, A. J. and Linder, J. A. (2016) Benzodiazepines are prescribed more frequently to patients already at risk for benzodiazepine-related adverse events in primary care. J. Gen. Intern. Med. 31, 1027-1034.   DOI
22 Lopez-Rodriguez, F., Wilson, C. L., Maidment, N. T., Poland, R. E. and Engel, J. (2003) Total sleep deprivation increases extracellular serotonin in the rat hippocampus. Neuroscience 121, 523-530.   DOI
23 Li, K. and Wang, H. (2004) Simultaneous determination of matrine, sophoridine and oxymatrine in Sophora flavescens Ait. by high performance liquid chromatography. Biomed. Chromatogr. 18, 178-182.   DOI
24 Lima, M. G., Bergamo Francisco, P. M. and de Azevedo Barros, M. B. (2012) Sleep duration pattern and chronic diseases in Brazilian adults (ISACAMP, 2008/09). Sleep Med. 13, 139-144.   DOI
25 Liu, Y., Xu, Y., Ji, W., Li, X., Sun, B., Gao, Q. and Su, C. (2014) Anti-tumor activities of matrine and oxymatrine: literature review. Tumour Biol. 35, 5111-5119.   DOI
26 Melancon, M. O., Lorrain, D. and Dionne, I. J. (2014) Exercise and sleep in aging: emphasis on serotonin. Pathol. Biol. (Paris) 62, 276-283.   DOI
27 Monti, J. M. (2011) Serotonin control of sleep-wake behavior. Sleep Med. Rev. 15, 269-281.   DOI
28 Monti, J. M. and Jantos, H. (2003) Differential effects of the 5-HT1A receptor agonist flesinoxan given locally or systemically on REM sleep in the rat. Eur. J. Pharmacol. 478, 121-130.   DOI
29 Murray, N. M., Buchanan, G. F. and Richerson, G. B. (2015) Insomnia caused by serotonin depletion is due to hypothermia. Sleep 38, 1985-1993.   DOI
30 Okuro, M., Fujiki, N., Kotorii, N., Ishimaru, Y., Sokoloff, P. and Nishino, S. (2010) Effects of paraxanthine and caffeine on sleep, locomotor activity, and body temperature in orexin/ataxin-3 transgenic narcoleptic mice. Sleep 33, 930-942.   DOI
31 Pariente, A., de Gage, S. B., Moore, N. and Begaud, B. (2016) The benzodiazepine-dementia disorders link: current state of knowledge. CNS Drugs 30, 1-7.   DOI