Browse > Article
http://dx.doi.org/10.4062/biomolther.2016.022

Baicalein Protects Human Skin Cells against Ultraviolet B-Induced Oxidative Stress  

Oh, Min Chang (School of Medicine, Jeju National University)
Piao, Mei Jing (School of Medicine, Jeju National University)
Jayatissa Fernando, Pattage Madushan Dilhara (School of Medicine, Jeju National University)
Han, Xia (School of Medicine, Jeju National University)
Madduma Hewage, Susara Ruwan Kumara (School of Medicine, Jeju National University)
Park, Jeong Eon (School of Medicine, Jeju National University)
Ko, Mi Sung (College of Medicine, Soonchunhyang University)
Jung, Uhee (Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute)
Kim, In Gyu (Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute)
Hyun, Jin Won (School of Medicine, Jeju National University)
Publication Information
Biomolecules & Therapeutics / v.24, no.6, 2016 , pp. 616-622 More about this Journal
Abstract
Baicalein (5,6,7-trihydroxy-2-phenyl-chromen-4-one) is a flavone, a type of flavonoid, originally isolated from the roots of Scutellaria baicalensis. This study evaluated the protective effects of baicalein against oxidative damage-mediated apoptosis induced by ultraviolet B (UVB) radiation in a human keratinocyte cell line (HaCaT). Baicalein absorbed light within the wavelength range of UVB. In addition, baicalein decreased the level of intracellular reactive oxygen species (ROS) in response to UVB radiation. Baicalein protected cells against UVB radiation-induced DNA breaks, 8-isoprostane generation and protein modification in HaCaT cells. Furthermore, baicalein suppressed the apoptotic cell death by UVB radiation. These findings suggest that baicalein protected HaCaT cells against UVB radiation-induced cell damage and apoptosis by absorbing UVB radiation and scavenging ROS.
Keywords
Baicalein; Ultraviolet B; Oxidative stress; Reactive oxygen species; HaCaT cells;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Beauchamp, M. C., Letendre, E. and Renier, G. (2002) Macrophage lipoprotein lipase expression is increased in patients with heterozygous familial hypercholesterolemia. J. Lipid Res. 43, 215-222.
2 Deschamps, J. D., Kenyon, V. A. and Holman, T. R. (2006) Baicalein is a potent in vitro inhibitor against both reticulocyte 15-human and platelet 12-human lipoxygenases. Bioorg. Med. Chem. 14, 4295-4301.   DOI
3 Dhumrongvaraporn, A. and Chanvorachote, P. (2013) Kinetics of ultraviolet B irradiation-mediated reactive oxygen species generation in human keratinocytes. J. Cosmet. Sci. 64, 207-217.
4 Hsieh, C. J., Hall, K., Ha, T., Li, C., Krishnaswamy, G. and Chi, D. S. (2007) Baicalein inhibits $IL-1{\beta}$- and $TNF-{\alpha}$-induced inflammatory cytokine production from human mast cells via regulation of the $NF-{\kappa}B$ pathway. Clin. Mol. Allergy 5, 5.   DOI
5 Jean-Denis, J. B., Pezet, R. and Tabacchi, R. (2006) Rapid analysis of stilbenes and derivatives from downy mildew-infected grapevine leaves by liquid chromatography-atmospheric pressure photoionisation mass spectrometry. J. Chromatogr. A 1112, 263-268.   DOI
6 Kim, K. C., Kang, S. S., Lee, J., Park, D. and Hyun, J. W. (2012a) Baicalein attenuates oxidative stress-induced expression of matrix metalloproteinase-1 by regulating the ERK/JNK/AP-1 pathway in human keratinocytes. Biomol. Ther. (Seoul) 20, 57-61.   DOI
7 Sen, S., Chakraborty, R., Sridhar, C., Reddy, Y. S. R. and De, B. (2010) Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. Int. J. Pharm. Sci. Rev. Res. 3, 91-100.
8 Li, L., Abe, Y., Kanagawa, K., Usui, N., Imai, K., Mashino, T., Mochizuki, M. and Miyata, N. (2004) Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method. Anal. Chim. Acta 512, 121-124.   DOI
9 Rosenkranz, A. R., Schmaldienst, S., Stuhlmeier, K. M., Chen, W., Knapp, W. and Zlabinger, G. J. (1992) A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate. J. Immunol. Methods 156, 39-45.   DOI
10 Schneider, L. A., Bloch, W., Kopp, K., Hainzl, A., Rettberg, P., Wlaschek, M., Horneck, G. and Scharffetter-Kochanek, K. (2006) 8-Isoprostane is a dose-related biomarker for photo-oxidative ultraviolet (UV) B damage in vivo: a pilot study with personal UV dosimetry. Br. J. Dermatol. 154, 1147-1154.   DOI
11 Sime, S. and Reeve, V. E. (2004) Protection from inflammation, immunosuppression and carcinogenesis induced by UV radiation in mice by topical $Pycnogenol^{(R)}$. Photochem. Photobiol. 79, 193-198.   DOI
12 Sklar, L. R., Almutawa, F., Lim, H. W. and Hamzavi, I. (2013) Effects of ultraviolet radiation, visible light, and infrared radiation on erythema and pigmentation: a review. Photochem. Photobiol. Sci. 12, 54-64.   DOI
13 Vera Saltos, M. B., Naranjo Puente, B. F., Milella, L., De Tommasi, N., Dal Piaz, F. and Braca, A. (2015) Antioxidant and free radical scavenging activity of phenolics from Bidens humilis. Planta Med. 81, 1056-1064.   DOI
14 Lee, S. J. and Park, J. W. (2014) Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP+-dependent isocitrate dehydrogenase in HaCaT cells. BMB Rep. 47, 209-214.   DOI
15 Kim, K. C., Lee, I. K., Kang, K. A., Kim, H. S., Kang, S. S. and Hyun, J. W. (2012b) Baicalein (5,6,7-trihydroxyflavone) reduces oxidative stress-induced DNA damage by upregulating the DNA repair system. Cell Biol. Toxicol. 28, 421-433.   DOI
16 Lee, I. K., Kang, K. A., Zhang, R., Kim, B. J., Kang, S. S. and Hyun, J. W. (2011) Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase. Environ. Toxicol. Pharmacol. 31, 233-241.   DOI
17 Lee, K.O., Kim, S. N. and Kim, Y. C. (2014) Anti-wrinkle effects of water extracts of teas in hairless mouse. Toxicol. Res. 30, 283-289.   DOI
18 Lin, Y. L., Lin, R. J., Shen, K. P., Dai, Z. K., Chen, I. J., Wu, J. R. and Wu, B. N. (2011) Baicalein, isolated from Scutellaria baicalensis, protects against endothelin-1-induced pulmonary artery smooth muscle cell proliferation via inhibition of TRPC1 channel expression. J. Ethnopharmacol. 138, 373-381.   DOI
19 Narayanan, D. L., Saladi, R. N. and Fox, J. L. (2010) Ultraviolet radiation and skin cancer. Int. J. Dermatol. 49, 978-986.   DOI
20 Markovic, J. M. D., Markovic, Z. S., Brdaric, T. P., Pavelkic, V. M. and Jadranin, M. B. (2011) Iron complexes of dietary flavonoids: Combined spectroscopic and mechanistic study of their free radical scavenging activity. Food Chem. 129, 1567-1577.   DOI
21 Okimoto, Y., Watanabe, A., Niki, E., Yamashita, T. and Noguchi, N. (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett. 474, 137-140.   DOI
22 Pallela, R., Na-Young, Y. and Kim, S. K. (2010) Anti-photoaging and photoprotective compounds derived from marine organisms. Mar. Drugs 8, 1189-1202.   DOI
23 Palmer, D. M. and Kitchin, J. S. (2010) Oxidative damage, skin aging, antioxidants and a novel antioxidant rating system. J. Drugs Dermatol. 9, 11-15.
24 Petrova, A., Davids, L. M., Rautenbach, F. and Marnewick, J. L. (2011) Photoprotection by honeybush extracts, hesperidin and mangiferin against UVB-induced skin damage in SKH-1 mice. J. Photochem. Photobiol. B, Biol. 103, 126-139.   DOI
25 Pirinccioglu, A. G., Gokalp, D., Pirinccioglu, M., Kizil, G. and Kizil, M. (2010) Malondialdehyde (MDA) and protein carbonyl (PCO) levels as biomarkers of oxidative stress in subjects with familial hypercholesterolemia. Clin. Biochem. 43, 1220-1224.   DOI
26 Rajagopalan, R., Ranjan, S. and Nair, C. K. (2003) Effect of vinblastine sulfate on gamma-radiation-induced DNA single-strand breaks in murine tissues. Mutat. Res. 536, 15-25.   DOI
27 Rastogi, P. R. and Madamwar, D. (2015) UV-Induced Oxidative Stress in Cyanobacteria: How life is able to survive? Biochem. Anal. Biochem. 4, 173.