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http://dx.doi.org/10.4062/biomolther.2015.094

Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice  

Chen, Ping (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University)
Chen, Yang (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University)
Wang, Yarong (Department of Pharmaceutical Sciences, Zunyi Medical University Zhuhai Campus)
Cai, Shining (Department of Pharmaceutical Sciences, Zunyi Medical University Zhuhai Campus)
Deng, Liang (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University)
Liu, Jia (Department of English, Zunyi Medical University Zhuhai Campus)
Zhang, Hao (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University)
Publication Information
Biomolecules & Therapeutics / v.24, no.2, 2016 , pp. 156-162 More about this Journal
Abstract
Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with $CCl_4$. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in $CCl_4$-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in $CCl_4$-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on $CCl_4$-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.
Keywords
Gardenia jasminoides Ellis; Hepatoprotective; Geniposide; Crocin-1; Crocetin; Histopathological examination;
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