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http://dx.doi.org/10.4062/biomolther.2015.165

Anti-Melanogenic Potentials of Nanoparticles from Calli of Resveratrol-Enriched Rice against UVB-Induced Hyperpigmentation in Guinea Pig Skin  

Lee, Taek Hwan (College of Pharmacy, Yonsei University)
Kang, Ji Hee (College of Pharmacy, Gachon University)
Seo, Jae Ok (College of Pharmacy, Gachon University)
Baek, So-Hyeon (National Institute of Crop Science, Rural Development Administration)
Moh, Sang Hyun (Anti-aging Research Institute, BIO-FD&C Co., LTD.)
Chae, Jae Kyoung (College of Pharmacy, Gachon University)
Park, Yong Un (College of Pharmacy, Gachon University)
Ko, Young Tag (College of Pharmacy, Gachon University)
Kim, Sun Yeou (College of Pharmacy, Gachon University)
Publication Information
Biomolecules & Therapeutics / v.24, no.1, 2016 , pp. 85-93 More about this Journal
Abstract
We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from RR into the skin. We aimed to develop anti-melanogenic agents incorporating calli of RR (cRR) and nanoparticles by high-pressure homogenization, examining the synergistic effects on the inhibition of UVB-induced hyperpigmentation. Depigmentation was observed following topical application of micro-cRR, nano-calli of normal rice (cNR), and nano-cRR to ultraviolet B (UVB)-stimulated hyperpigmented guinea pig dorsal skin. Colorimetric analysis, tyrosinase immunostaining, and Fontana-Masson staining for UVB-promoted melanin were performed. Nano-cRR inhibited changes in the melanin color index caused by UVB-promoted hyperpigmentation, and demonstrated stronger anti-melanogenic potential than micro-cRR. In epidermal skin, nano-cRR repressed UVB-promoted melanin granules, thereby suppressing hyperpigmentation. The UVB-enhanced, highly expressed tyrosinase in the basal layer of the epidermis was inhibited by nano-cRR more prominently than by micro-cRR and nano-cNR. The anti-melanogenic potency of nano-cRR also depended on pH and particle size. Nano-cRR shows promising potential to regulate skin pigmentation following UVB exposure.
Keywords
Plant tissue culture; High-pressure homogenization; Melanogenesis; Resveratrol-enriched rice; Nanoparticles;
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Times Cited By KSCI : 4  (Citation Analysis)
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