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http://dx.doi.org/10.4062/biomolther.2015.023

Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells  

Jeong, Kyu-Tae (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Lee, Eujin (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Park, Na-Young (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Kim, Sun-Gun (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Park, Hyo-Hyun (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Lee, Jiean (R&D Center, Morechem Co., Ltd.)
Lee, Youn Ju (School of Medicine, Catholic University of Daegu)
Lee, Eunkyung (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
Publication Information
Biomolecules & Therapeutics / v.23, no.5, 2015 , pp. 421-427 More about this Journal
Abstract
Imperatorin has been known to exert many biological functions including anti-inflammatory activity. In this study, we investigated the inhibitory effects of imperatorin on the production of inflammatory mediators in mouse bone marrow-derived mast cells (BMMC). Imperatorin inhibited degranulation and the generation of eicosanoids (leukotriene $C_4$ ($LTC_4$) and prostaglandin $D_2$ ($PGD_2$) in IgE/antigen (Ag)-stimulated BMMC. To elucidate the molecular mechanism involved in this process, we investigated the effect of imperatorin on intracellular signaling in BMMC. Biochemical analyses of the IgE/Ag-mediated signaling pathway demonstrated that imperatorin dramatically attenuated degranulation and the production of 5-lipoxygenase-dependent $LTC_4$ and cyclooxygenase-2-dependent $PGD_2$ through the inhibition of intracellular calcium influx/phospholipase $C{\gamma}1$, cytosolic phospholipase $A_2$/mitogen-activated protein kinases and/or nuclear factor-${\kappa}B$ pathways in BMMC. These results suggest that the effects of imperatorin on inhibition of degranulation and eicosanoid generation through the suppression of multiple steps of IgE/Ag-mediated signaling pathways would be beneficial for the prevention of allergic inflammation.
Keywords
Leukotriene $C_4$; Prostaglandin $D_2$; Cytosolic phospholipase $A_2$; Mitogen-activated protein kinases; Phospholipase $C{\gamma}1$;
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