Browse > Article
http://dx.doi.org/10.4062/biomolther.2014.141

Dieckol, a Component of Ecklonia cava, Suppresses the Production of MDC/CCL22 via Down-Regulating STAT1 Pathway in Interferon-γ Stimulated HaCaT Human Keratinocytes  

Kang, Na-Jin (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Koo, Dong-Hwan (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Kang, Gyeoung-Jin (Department of Medicine, School of Medicine, Jeju National University)
Han, Sang-Chul (Department of Medicine, School of Medicine, Jeju National University)
Lee, Bang-Won (Department of Medicine, School of Medicine, Jeju National University)
Koh, Young-Sang (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Hyun, Jin-Won (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Lee, Nam-Ho (Department of Chemistry, College of Natural Science, Jeju National University)
Ko, Mi-Hee (Jeju Biodiversity Research Institute)
Kang, Hee-Kyoung (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Yoo, Eun-Sook (Department of Biomedicine & Drug Development, School of Medicine, Jeju National University)
Publication Information
Biomolecules & Therapeutics / v.23, no.3, 2015 , pp. 238-244 More about this Journal
Abstract
Macrophage-derived chemokine, C-C motif chemokine 22 (MDC/CCL22), is one of the inflammatory chemokines that controls the movement of monocytes, monocyte-derived dendritic cells, and natural killer cells. Serum and skin MDC/CCL22 levels are elevated in atopic dermatitis, which suggests that the chemokines produced from keratinocytes are responsible for attracting inflammatory lymphocytes to the skin. A major signaling pathway in the interferon-${\gamma}$ (IFN-${\gamma}$)-stimulated inflammation response involves the signal transducers and activators of transcription 1 (STAT1). In the present study, we investigated the anti-inflammatory effect of dieckol and its possible action mechanisms in the category of skin inflammation including atopic dermatitis. Dieckol inhibited MDC/CCL22 production induced by IFN-${\gamma}$ (10 ng/mL) in a dose dependent manner. Dieckol (5 and $10{\mu}M$) suppressed the phosphorylation and the nuclear translocation of STAT1. These results suggest that dieckol exhibits anti-inflammatory effect via the down-regulation of STAT1 activation.
Keywords
Dieckol; Inflammation; Keratinocyte; MDC/CCL22; STAT1;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Qi, X. F., Kim, D. H., Yoon, Y. S., Li, J. H., Jin, D., Teng, Y. C., Kim, S. K. and Lee, K. J. (2009) Fluvastatin inhibits expression of the chemokine MDC/CCL22 induced by interferon-gamma in HaCaT cells, a human keratinocyte cell line. Br. J. Pharmacol. 157, 1441-1450.   DOI
2 Rauch, I., Muller, M. and Decker, T. (2013) The regulation of inflammation by interferons and their STATs. JAKSTAT 2, e23820.
3 Saeki, H. and Tamaki, K. (2006) Thymus and activation regulated chemokine (TARC)/CCL17 and skin diseases. J. Dermatol. Sci. 43, 75-84.   DOI
4 Semper, C., Leitner, N. R., Lassnig, C., Parrini, M., Mahlakoiv, T., Rammerstorfer, M., Lorenz, K., Rigler, D., Muller, S., Kolbe, T., Vogl, C., Rulicke, T., Staeheli, P., Decker, T., Muller, M. and Strobl, B. (2014) STAT1beta is not dominant negative and is capable of contributing to gamma interferon-dependent innate immunity. Mol. Cell. Biol. 34, 2235-2248.   DOI
5 Shimada, Y., Takehara, K. and Sato, S. (2004) Both Th2 and Th1 chemokines (TARC/CCL17, MDC/CCL22, and Mig/CXCL9) are elevated in sera from patients with atopic dermatitis. J. Dermatol. Sci. 34, 201-208.   DOI
6 van den Broek, M. F., Muller, U., Huang, S., Zinkernagel, R. M. and Aguet, M. (1995) Immune defence in mice lacking type I and/or type II interferon receptors. Immunol. Rev. 148, 5-18.   DOI
7 Wijesinghe, W. A., Ko, S. C. and Jeon, Y. J. (2011) Effect of phlorotannins isolated from Ecklonia cava on angiotensin I-converting enzyme (ACE) inhibitory activity. Nutr. Res. Pract. 5, 93-100.   DOI
8 Yamashita, U. and Kuroda, E. (2002) Regulation of macrophage-derived chemokine (MDC, CCL22) production. Crit. Rev. Immunol. 22, 105-114.
9 Yayeh, T., Im, E. J., Kwon, T. H., Roh, S. S., Kim, S., Kim, J. H., Hong, S. B., Cho, J. Y., Park, N. H. and Rhee, M. H. (2014) Hemeoxygenase 1 partly mediates the anti-inflammatory effect of dieckol in lipopolysaccharide stimulated murine macrophages. Int. Immunopharmacol. 22, 51-58.   DOI
10 Yoon, W. J., Kim, K. N., Heo, S. J., Han, S. C., Kim, J., Ko, Y. J., Kang, H. K. and Yoo, E. S. (2013) Sargachromanol G inhibits osteoclastogenesis by suppressing the activation NF-kappaB and MAPKs in RANKL-induced RAW 264.7 cells. Biochem. Biophys. Res. Commun. 434, 892-897.   DOI
11 Yoshie, O., Imai, T. and Nomiyama, H. (2001) Chemokines in immunity. Adv. Immunol. 78, 57-110.   DOI
12 Farrar, M. A. and Schreiber, R. D. (1993) The molecular cell biology of interferon-gamma and its receptor. Annu. Rev. Immunol. 11, 571-611.   DOI
13 Baumer, W., Seegers, U., Braun, M., Tschernig, T. and Kietzmann, M. (2004) TARC and RANTES, but not CTACK, are induced in two models of allergic contact dermatitis. Effects of cilomilast and diflorasone diacetate on T-cell-attracting chemokines. Br. J. Dermatol. 151, 823-830.   DOI
14 Best, S. M., Morris, K. L., Shannon, J. G., Robertson, S. J., Mitzel, D. N., Park, G. S., Boer, E., Wolfinbarger, J. B. and Bloom, M. E. (2005) Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. J. Virol. 79, 12828-12839.   DOI
15 Chae, D., Manzoor, Z., Kim, S. C., Kim, S., Oh, T. H., Yoo, E. S., Kang, H. K., Hyun, J. W., Lee, N. H., Ko, M. H. and Koh, Y. S. (2013) Apo-9'-fucoxanthinone, isolated from Sargassum muticum, inhibits CpG-induced inflammatory response by attenuating the mitogenactivated protein kinase pathway. Mar. Drugs 11, 3272-3287.   DOI
16 Choi, H. J., Park, J. H., Lee, B. H., Chee, H. Y., Lee, K. B. and Oh, S. M. (2014) Suppression of NF-kappaB by dieckol extracted from Ecklonia cava negatively regulates LPS induction of inducible nitric oxide synthase gene. Appl Biochem Biotechnol 173, 957-967.   DOI
17 Decker, T. and Kovarik, P. (2000) Serine phosphorylation of STATs. Oncogene 19, 2628-2637.   DOI
18 Han, S. K., Song, J. Y., Yun, Y. S. and Yi, S. Y. (2002) Gamma irradiation-reduced IFN-gamma expression, STAT1 signals, and cellmediated immunity. J. Biochem. Mol. Biol. 35, 583-589.   DOI
19 Ivashkiv, L. B. and Hu, X. (2004) Signaling by STATs. Arthritis Res. Ther. 6, 159-168.   DOI
20 Jahnz-Rozyk, K., Targowski, T., Paluchowska, E., Owczarek, W. and Kucharczyk, A. (2005) Serum thymus and activation-regulated chemokine, macrophage-derived chemokine and eotaxin as markers of severity of atopic dermatitis. Allergy 60, 685-688.   DOI
21 Ju, S. M., Song, H. Y., Lee, S. J., Seo, W. Y., Sin, D. H., Goh, A. R., Kang, Y. H., Kang, I. J., Won, M. H., Yi, J. S., Kwon, D. J., Bae, Y. S., Choi, S. Y. and Park, J. (2009) Suppression of thymus- and activation-regulated chemokine (TARC/CCL17) production by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose via blockade of NF-kappaB and STAT1 activation in the HaCaT cells. Biochem. Biophys. Res. Commun. 387, 115-120.   DOI
22 Kang, S. M., Heo, S. J., Kim, K. N., Lee, S. H., Yang, H. M., Kim, A. D. and Jeon, Y. J. (2012b) Molecular docking studies of a phlorotannin, dieckol isolated from Ecklonia cava with tyrosinase inhibitory activity. Bioorg. Med. Chem. 20, 311-316.   DOI   ScienceOn
23 Jung, H. A., Jin, S. E., Ahn, B. R., Lee, C. M. and Choi, J. S. (2013) Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages. Food Chem. Toxicol. 59, 199-206.   DOI   ScienceOn
24 Kang, M. C., Ahn, G., Yang, X., Kim, K. N., Kang, S. M., Lee, S. H., Ko, S. C., Ko, J. Y., Kim, D., Kim, Y. T., Jee, Y., Park, S. J. and Jeon, Y. J. (2012a) Hepatoprotective effects of dieckol-rich phlorotannins from Ecklonia cava, a brown seaweed, against ethanol induced liver damage in BALB/c mice. Food Chem. Toxicol. 50, 1986-1991.   DOI   ScienceOn
25 Kang, M. C., Wijesinghe, W. A., Lee, S. H., Kang, S. M., Ko, S. C., Yang, X., Kang, N., Jeon, B. T., Kim, J., Lee, D. H. and Jeon, Y. J. (2013) Dieckol isolated from brown seaweed Ecklonia cava attenuates type capital I, Ukrainiancapital I, Ukrainian diabetes in db/db mouse model. Food Chem. Toxicol. 53, 294-298.   DOI   ScienceOn
26 Kang, G. J., Lee, H. J., Yoon, W. J., Yang, E. J., Park, S. S., Kang, H. K., Park, M. H. and Yoo, E. S. (2008) Prunus yedoensis inhibits the inflammatory chemokines, MDC and TARC, by regulating the STAT1-signaling pathway in IFN-${\gamma}$-stimulated HaCaT human keratinocytes. Biomol. Ther. 16, 394-402.   DOI
27 Kim, S. K. and Himaya, S. W. (2011) Medicinal effects of phlorotannins from marine brown algae. Adv. Food Nutr. Res. 64, 97-109.   DOI
28 Madonna, S., Scarponi, C., De Pita, O. and Albanesi, C. (2008) Suppressor of cytokine signaling 1 inhibits IFN-gamma inflammatory signaling in human keratinocytes by sustaining ERK1/2 activation. FASEB J. 22, 3287-3297.   DOI
29 Kim, K. C., Piao, M. J., Zheng, J. Y., Cheng, W., Cha, J. W., Kumara, M. H. S. R., Han, Xia., Kang, H. K., Lee, N. H. and Hyun, J. W. (2014) Fucodiphlorethol G purified from Ecklonia cava suppresses ultraviolet B radiation-induced oxidative stress and cellular damage. Biomol. Ther. 22, 301-307.   DOI
30 Lee, S. H., Park, M. H., Kang, S. M., Ko, S. C., Kang, M. C., Cho, S., Park, P. J., Jeon, B. T., Kim, S. K., Han, J. S. and Jeon, Y. J. (2012) Dieckol isolated from Ecklonia cava protects against high-glucose induced damage to rat insulinoma cells by reducing oxidative stress and apoptosis. Biosci. Biotechnol. Biochem. 76, 1445-1451.   DOI
31 Maeda, S., Fujiwara, S., Omori, K., Kawano, K., Kurata, K., Masuda, K., Ohno, K. and Tsujimoto, H. (2002) Lesional expression of thymus and activation-regulated chemokine in canine atopic dermatitis. Vet. Immunol. Immunopathol. 88, 79-87.   DOI
32 Park, J. Y., Kim, J. H., Kwon, J. M., Kwon, H. J., Jeong, H. J., Kim, Y. M., Kim, D., Lee, W. S. and Ryu, Y. B. (2013) Dieckol, a SARS-CoV 3CL(pro) inhibitor, isolated from the edible brown algae Ecklonia cava. Bioorg. Med. Chem. 21, 3730-3737.   DOI
33 Park, S. J. and Jeon, Y. J. (2012) Dieckol from Ecklonia cava suppresses the migration and invasion of HT1080 cells by inhibiting the focal adhesion kinase pathway downstream of Rac1-ROS signaling. Mol. Cells 33, 141-149.   DOI
34 Pearson, G., Robinson, F., Beers Gibson, T., Xu, B. E., Karandikar, M., Berman, K. and Cobb, M. H. (2001) Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22, 153-183.